Hl 60

The promyelocytic leukemia HL-60 and breast cancer adenocarcinoma MCF-7 cell lines were purchased from the European Collection of Cell Cultures (ECACC). Leukemia cells were cultured in RPMI 1640 plus GlutaMax I medium (Gibco/Life Technologies, Carlsbad, CA, USA). MCF-7 cells were maintained in Minimum Essential Medium Eagle (Sigma Aldrich, St. Louis, MO, USA) and supplemented with 2 mM glutamine and men non-essential amino acid solution (Sigma Aldrich, St. Louis, MO, USA). Both media were supplemented with 10% heat-inactivated fetal bovine serum (Biological Industries, Beit-Haemek, Israel) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) (Sigma-Aldrich, St. Louis, MO, USA). Human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC). Cells were cultured using EGM-2 Endothelial Medium BulletKit purchased from Lonza (Lonza, Walkersville, MD, USA). Cells were maintained at 37 °C in 5% CO₂ atmosphere and grown until 80% confluent.
The tested compounds were dissolved in sterile dimethyl sulfoxide (DMSO) and further diluted with culture medium. The final concentration of DMSO in cell cultures was less than 0.1% v/v. Controls without and with 0.1% DMSO were performed in each experiment. At the used concentration, DMSO had no effect on the observed parameters.

HUT78 and Lu1205 cells were provided by Drs A. Marie-Cardine and N. Dumaz and genotyped to verify their authenticity. MRC-5 and U2OS cells were provided by Drs M. Dutreix and R. Fåhraeus, and were purchased from ATCC. HaCat cells were provided by Prof. N. Basset-Seguin and their characteristics were described elsewhere [25 (link)]. Jurkat and HL-60 were purchased from The European Collection of Cell Cultures. Cells were cultivated either in DMEM or RPMI 1640 (Life Technologies), supplemented with 10% FBS and 1% penicillin/streptomycin. Cytochemistry analysis were performed as previously described [26 (link), 27 (link)]. All chemicals were purchased from Sigma.