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Goat anti brn1

Manufactured by Novus Biologicals

Goat anti-Brn1 is a primary antibody designed to detect the Brn1 (POU3F3) protein in various research applications. It is produced in goats and can be used for techniques such as Western blotting, immunohistochemistry, and immunocytochemistry.

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2 protocols using goat anti brn1

1

Immunolabeling of Embryonic Neural Cells

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Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously (Kim et al., 2009 ). The following primary antibodies were used: rabbit anti-MACF1 (Wu et al., 2011 (link)), rabbit anti-MACF1 (Santa Cruz), rabbit anti-phospho-MACF1 (Wu et al., 2011 (link)), chicken anti-nestin (Neuromics), mouse anti-MAP2 (Covance), rabbit anti-Tbr1 (Chemicon), rabbit anti-Cux1 (Santa Cruz), goat anti-Brn1 (Novus Biologicals), rabbit anti-acetyl-α-tubulin (Cell Signaling), mouse anti-α-tubulin (Sigma), and chicken anti-actin (Millipore). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies.
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2

Immunohistochemical Profiling of Neural Cells

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Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously (Kim et al., 2006 (link)). The following primary antibodies were used: rabbit anti-mTOR (Cell Signaling), rabbit anti-4EBP1 (Cell Signaling), mouse anti-S6 (Cell Signaling), rabbit anti-TSC2 (Cell Signaling), rabbit anti-phospho-mTOR (Cell Signaling), rabbit anti-phospho-4EBP1 (Cell Signaling), rabbit anti-phospho-S6 (Cell Signaling), rabbit anti-phospho-S6K (Cell Signaling), rabbit anti-phospho-histone H3 (Upstate Biotech), mouse anti-BrdU (Sigma), rabbit anti-Ki67 (Covance), rabbit anti-Sox2 (Chemicon), rabbit anti-Tbr1 (Chemicon), rabbit anti-Cux1 (Santa Cruz), goat anti-Brn1 (Novus Biologicals), rabbit anti-Tbr2 (Abcam), mouse anti-Tuj1 (Sigma). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies.
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