Cd163

Manufactured by Bioss Antibodies

Sourced in United States

CD163 is a cell surface receptor that is primarily expressed on the surface of monocytes and macrophages. It functions as a scavenger receptor, playing a role in the clearance of hemoglobin-haptoglobin complexes from the bloodstream.

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Lab products found in correlation

Phospho erk, by Cell Signaling Technology (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Bradford protein assay kit, by Avantor (1 mentions) N cadherin, by Abcam (1 mentions) Gapdh, by Syngene (1 mentions) Cola1, by Santa Cruz Biotechnology (1 mentions) α sma, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Genetools software, by Syngene (1 mentions) Hnf4a, by Santa Cruz Biotechnology (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) F4 80, by Thermo Fisher Scientific (1 mentions) Tissue tek oct compound, by Sakura Finetek (1 mentions) Tunel assay, by Roche (1 mentions) Ix71 microscope, by Olympus (1 mentions) Epcam, by BD (1 mentions) Envision kit, by Agilent Technologies (1 mentions) Metamorph, by Molecular Devices (1 mentions) Anti cd45 antibody, by BD (1 mentions) Ix81 microscope, by Olympus (1 mentions)

Close spelling variants of this product

Rabbit anti-CD163 (3 citations) Anti-CD163 (3 citations) Mouse anti-mouse CD163 (1 citations)

5 protocols using cd163

Immunohistochemical Analysis of Liver Tissue

Cited 1 time

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Liver tissue was fixed in z-Fix solution or embedded in Tissue-Tek OCT compound (Sakura Finetek) for paraffin and frozen block preparation, respectively. Paraffin sections were stained for F4/80 (ebioscience), CD163 (Bioss), PCNA (Santa Cruz), p21 (Santa Cruz), A6 (DSH) and SOX9 (BD Bioscience). Frozen tissue sections were stained for EpCam (BD Bioscience), NK1.1 (BioLegend), F4/80 (ebioscience), CD163 (Bioss), iNOS (abcam), HNF4a (Santa Cruz) and TUNEL assay according to the manufacturer’s procedures (Roche). The images were acquired with an Olympus IX71 microscope and CellSense software. For Oil Red-O staining, frozen sections were fixed with 4% PFA in PBS for 10 minutes at room temperature. Slides were washed twice with 1X PBS and permeabilized with 1X PBST for 5 minutes at room temperature with gentle rocking. Slides were washed three times with 1X PBS for 10 minutes each at room temperature, and stained with Oil Red-O solution (4 g/L ORO powder in 60% isopropanol) at 56 °C for 40 min. Slides were washed twice with 1X PBS and counterstained with Vectshield with DAPI mounting medium (Vector Labs). SA-β-gal staining was performed as previously described (Krizhanovsky et al., 2008 (link)).

Lee J., Liao R., Wang G., Yang B.H., Luo X., Varki N.M., Qiu S.J., Ren B., Fu W, & Feng G.S. (2017). Preventive Inhibition of Liver Tumorigenesis by Systemic Activation of Innate Immune Functions. Cell reports, 21(7), 1870-1882.

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Wound Healing Histopathological Analysis

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On postoperative days 5, 7, 14, 21 and 35, the wound and surrounding tissue (0.5 cm) were carefully excised, rinsed in PBS, and then fixed in 4% Paraformaldehyde (PFA). Samples were dehydrated in a graded ethanol series (70–100%) and embedded in paraffin. Five-micrometer sections were prepared. According to the standard procedures, samples were stained with either Hematoxylin and Eosin (HE) or Masson trichrome, and immunohistochemistry including α-SMA (1:100, Boster), K1 (1:100, Abcam), K6 (1:500, Covance), CD86 and CD163 (1:200, Bioss)38 (link).
Image-Pro Plus was used to analyse the average optical density values for K1, K6, α-SMA expression. Five randomly selected fields of view were examined for each group at each time point and used to assess the average optical density value per unit area. Statistics regarding the number of positively stained cells for CD86 and CD163 were obtained using five randomly selected fields of view for each group at each time point.

Chen S., Shi J., Zhang M., Chen Y., Wang X., Zhang L., Tian Z., Yan Y., Li Q., Zhong W., Xing M., Zhang L, & Zhang L. (2015). Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing. Scientific Reports, 5, 18104.

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Quantitative Protein Analysis of Skin

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Western blot analysis was applied to perform and quantify the amount of protein. The protein obtained from the skin was isolated using 1× cell lysis buffer (Cell Signaling, Danvers, MA, USA) and the concentration was determined with the Bradford Protein Assay Kit (AMRESCO, Solon, OH, USA). The specific antibodies used in the current study were listed as follows: α-SMA (Santa Cruz, Dallas, TX, USA, sc-32251), COLA1 (Santa Cruz, sc-8784), CD68 (Santa Cruz, sc-20060) and GAPDH (Santa Cruz, sc-25778), N-cadherin (Epitomics, Burlingame, CA, USA, 2019-1), ZO-1 (Cell Signaling, 9782), phospho-ERK (Cell Signaling, 9101) and ERK (Cell Signaling, 4695), activin A (myBioSource, San Diego, CA, USA, MBS7103066 & MBS9201920), and CD163 (Bioss, Woburn, MA, USA, bs-2527R). The band intensity was quantified by using GeneTools software (Syngene, Cambridge, UK) and the level of GAPDH was performed as internal control [44 (link)]. All experiments were performed in biological triplicate to confirm the reproducibility.

Wang P.W., Lin T.Y., Yang P.M., Yeh C.T, & Pan T.L. (2022). Hepatic Stellate Cell Modulates the Immune Microenvironment in the Progression of Hepatocellular Carcinoma. International Journal of Molecular Sciences, 23(18), 10777.

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Histological Analysis of Muscle Tissue

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The frozen tissues were fixed in paraformaldehyde (4% w/v) and transferred into ethanol (70% w/v). The samples were then paraffin embedded and sectioned using a microtome. Muscle sections were then stained with Hematoxylin and Eosin (H&E) stain for anatomical features.^{[9 (link)]} The samples were also immunostained using antibodies staining for von Willebrand factor (1:1000; DAKO), CD86 (1:250; Bioss) and CD163 (1:100; Bioss). The staining was detected using DAKO Envision kit using a 3,3′-di-amino benzidine (DAB) substrate. The vessel density was quantified by manually measuring the positively stained cells on Metamorph (Molecular Devices). Cells positive for CD86 or CD163 positive cells were quantified using the online tool “ImmunoRatio,” an automated image analysis software for DAB immunostained images.^{[16 (link)]}

Das S., Monteforte A.J., Singh G., Majid M., Sherman M.B., Dunn A.K, & Baker A.B. (2016). Syndecan-4 Enhances Therapeutic Angiogenesis after Hind limb Ischemia in Mice with Type 2 Diabetes. Advanced healthcare materials, 5(9), 1008-1013.

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Comprehensive Cardiac Histology Protocol

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Hearts were harvested and immediately immersed in 10% formalin for 48 h at 4 °C. Hearts were then embedded in paraffin and sectioned in 5 μm-thick transverse slices. After deparaffinisation and rehydration, sections were stained with haematoxylin-eosin (H&E), Prussian blue and Picrosirius red.
Cardiomyocytes were stained with an anti-troponin I antibody (Abcam, Cambridge, UK). The thickness of the infarct was evaluated in representative slices taken in the middle of the heart by measuring the endocardium to epicardium distance using ImageJ software (NIH, Bethesda, MD). Rhodamine-conjugated wheat germ agglutinin (WGA) was used to outline cell membranes. Large vessels and capillaries were labelled with anti-sm22α and isolectin B4 antibodies, respectively. Leukocytes were labelled using an anti-CD45 antibody (BD Biosciences, USA), detected with an HRP/DAB system followed by haematoxylin counterstaining. A similar procedure was used to detect CD163 (Bioss Inc., USA), a receptor involved in clearance and endocytosis of haemoglobin/haptoglobin complexes by macrophages [18] (link), and VCAM-1.
Imaging was performed on an Olympus IX-81 microscope. Quantification was performed in a blinded fashion, using Volocity® software (PerkinElmer, USA).

Protti A., Mongue-Din H., Mylonas K.J., Sirker A., Sag C.M., Swim M.M., Maier L., Sawyer G., Dong X., Botnar R., Salisbury J., Gray G.A, & Shah A.M. (2016). Bone marrow transplantation modulates tissue macrophage phenotype and enhances cardiac recovery after subsequent acute myocardial infarction. Journal of Molecular and Cellular Cardiology, 90, 120-128.

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