454 gs flx sequencer
The 454 GS-FLX sequencer is a next-generation DNA sequencing system developed by Roche. It utilizes pyrosequencing technology to enable rapid and high-throughput sequencing of DNA samples. The core function of the 454 GS-FLX sequencer is to perform DNA sequencing, providing researchers with genomic data for various applications.
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25 protocols using 454 gs flx sequencer
Antennal Transcriptome Sequencing
RNA Extraction and Normalization for Transcriptome Sequencing
A pooled RNA sample was provided to GATC Biotech (Konstanz, Germany) for the construction of a cDNA library and subsequent sequencing. Library construction involved DNAselection for polyadenylated (polyA+) transcripts to enrich for protein-coding mRNAs, DNase I treatment and normalization through denaturation/reassociation of cDNA, to improve the representation of low-copy transcripts and thereby maximize gene discovery. The resulting library was sequenced with a full picotiter plate on a 454 GS-FLX sequencer with Titanium reagents (Roche Applied Science, Indianapolis, IN, USA), using standard 454 protocols.
Targeted Sequencing of Salmonella Virulence Genes
Genome Assembly of Lactobacillus reuteri
454 Sequencing of GcM5-1A Genome
16S rRNA Microbiome Profiling of Armadillidium
Genome Sequencing of Bradyrhizobium sp. SUTN9-2
The genome sequences of Bradyrhizobium sp. SUTN9-2 (accession number LAXE00000000) were compared with the whole genome of B. diazoefficiens USDA110 (33 (link)) using the program GenomeMatcher (21 (link)) at the amino acid level. The annotated genome sequences of USDA110 (accession number BA000040) were obtained from Genome Assembly/Annotation Projects (NCBI database). The sequences of chromosome and plasmid pDOA9 are available in the DDBJ/GenBank/EMBL database (accession numbers DF820425 and DF820426, respectively).
Bacterial Diversity Analysis via 16S rRNA Sequencing
Tiger Shark DNA Sequencing and Microsatellite Isolation
To isolate microsatellites and design primers for population genetics all sequences of the SSR were compiled using Primer3 (Rozen & Skaletsky, 1999 ) and BatchPrimer3 (You et al., 2008 (link)). Primers were designed based on the following criteria: primer size of 20 bp (min = 18, max = 22 bp), ideal annealing temperature of 60 °C (min = 55 °C, max = 63 °C), GC optimum of 60% (min = 40%, max = 80%) and the size of the amplified product ranging from 50–500 bp. The sequences were then grouped and aligned in the Clustal W software (Thompson, Higgins & Gibson, 1994 (link)), identifying duplicated sequences for the same locus.
Characterization of TpUB05 Gene Sequence
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