The largest database of trusted experimental protocols

Chlorophyll meter

Manufactured by Konica Minolta
Sourced in Japan

The Chlorophyll Meter is a compact, handheld device designed to measure the chlorophyll content in leaves. It provides a non-destructive way to assess the plant's health and nutritional status. The device uses light absorption techniques to determine the chlorophyll concentration, which can be used as an indicator of the plant's photosynthetic capacity.

Automatically generated - may contain errors

13 protocols using chlorophyll meter

1

Leaf Morphology and Pigment Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leaf chlorophyll concentration: It was determined by using a chlorophyll measurement device (Chlorophyll meter, SPAD-502, Konica Minolta).
3. Leaf fresh weight and Leaf dry weight (g.Leaf -1 ): Leaf fresh weight and Leaf dry weight were determined according to the method described by Al-Sahaf (6) and Gobara (13) .
4. Petiole length (cm): by using metric tapeline line.
5. Internodes length (cm): by using metric tapeline.
6. Shoot length (cm): by using metric tape.
+ Open protocol
+ Expand
2

Leaf Pigment Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
At harvest, three 3rd fully expanded leaves from each replicate were used to estimate leaf SPAD value (chlorophyll index) and pigment contents. SPAD value was recorded just before the final harvest with a Chlorophyll Meter (Model: SPAD-502, Minolta Co., Ltd., Tokyo, Japan). Then, the leaves were harvested into Ziplock polybags and brought to the laboratory for pigment extraction. Leaf pigments were extracted in 80% (v/v) acetone and absorbance of the supernatant was determined with a UV-visible spectrophotometer (GENESYS 10S UV-VIS, Thermo Fisher Scientific, Waltham, MA, USA) at 663, 645, and 470 nm for Chl a, Chl b, and carotenoid content, respectively, and calculated according to Arnon [33 (link)].
+ Open protocol
+ Expand
3

Onion Growth and Soil Mite Abundance

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the effects on onion plant growth, five leaves were selected from the central third of each plant and treatment to measure the chlorophyll content (Minolta SPAD 502 plus chlorophyllmeter) every 15 d, recording the average per plant, expressed in SPAD Units. At week 14, when maturity was reached, the foliar area was measured, expressed in cm 2 were done, with a CI 202, Bio-Science Inc. The fresh and dry plant weights were quantified using an electronic balance (Acculab VIC 612 -0,01 g precision). The plants were dried for 48 h at 70ºC in a Memmert oven.
In addition, soil samples were taken with a metal cylinder,10 cm diameter and 5 cm depth. The samples were transported in plastic bags to the laboratory, where the mites were extracted with a modified Berlesse funnel (Oliveira et al., 2000) . Each sample was observed with a stereomicroscope (Leica microsystems) to confirm the presence of the Mesostigmata mites. The variables were measured in the laboratories of Biological control and Plant physiology of the UPTC.
The data were subjected to normality and variance homogeneity tests using Shapiro-Wilk and Bartlett tests, respectively. When the assumptions were verified, the variables that had statistical differences were tested with Tukey's comparison of means (P≤0.05). The analyses were carried out in R (version 3.3.2).
+ Open protocol
+ Expand
4

Chlorophyll and Photosynthesis in Maize

Check if the same lab product or an alternative is used in the 5 most similar protocols
The chlorophyll content and photosynthetic rate in maize leaves were determined by a chlorophyll meter (Konica Minolta Investment Ltd., Tokyo, Japan) and photosynthetic rate apparatus (Li-6400, LI-COR, Lincoln, NE, USA), respectively. All the above indexes were determined from the top leaf of the plant, nine strains were selected for each treatment and the average value was taken.
+ Open protocol
+ Expand
5

Leaf Chlorophyll Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chlorophyll Content (%) In leaves, The chlorophyll content was calculated for fully grown leaves of five plants from each experimental unit in the inner rows through the use of (Chlorophyll Meter, SPAD-502, Konica Minolta) and the average was accounted.
+ Open protocol
+ Expand
6

Leaf Chlorophyll Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seven and 14 days after planting, the leaf chlorophyll content was estimated by taking the mean of three readings with a portable Chlorophyll meter (Minolta Co. Ltd, Osaka, Japan).
+ Open protocol
+ Expand
7

Leaf Cross-Cut Structure Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For study of leaf crosscut structures, four leaves segments 3 mm long were cut from the middle of the second leaf from top in each treatment and were fixed in 2.5% glutaraldehyde (v/v) in 25 mol m-3 phosphate buffer at pH 7.0 (Huang and Fry, 1998 (link)), dehydrated in an ethanol series, critical-point dried with liquid CO2, and sputter-coated with gold-palladium. The xylem and phloem tissue, along with the sieve diameter and vascular circumference of the leaf segments were visually examined and photographed with the scanning electron microscope (S-4800, Japan) at 1000×. Ramets greenness (or relative chlorophyll content) was determined using a hand-held chlorophyll meter (measured as the optical density, SPAD reading, Minolta Camera Co., Osaka, Japan) in both homogenous and heterogeneous plots.
+ Open protocol
+ Expand
8

Evaluating Lettuce Chlorophyll and Water Use

Check if the same lab product or an alternative is used in the 5 most similar protocols
The harvest took place when the plants reached the marketable size (67–70 days after transplanting in pots). Chlorophyll content was measured immediately before harvest using SPAD-502 portable nondestructive chlorophyll meter (Konica Minolta Inc., Tokyo, Japan). Chlorophyll measurement was performed on a single fully developed leaf in the middle of lettuce head. After harvesting, plant vegetative growth parameters (e. g., fresh and dry weight of aerial part, root wet and dry weight, and number of leaves) were measured and recorded. Dry weights were determined after oven drying at 70 °C until constant weight is reached.
Water use efficiency (WUE) (kg m−3) indicates the biomass produced per unit of water used by the crop. It was evaluated as the ratio between the marketable yield (kg ha−1) and seasonal crop evapotranspiration (mm).
+ Open protocol
+ Expand
9

Phenotypic Analysis of CaRLP-Silenced Pepper Plants

Check if the same lab product or an alternative is used in the 5 most similar protocols
The morphological changes of each CaRLP-silenced pepper plant were measured, such as plant height (from soil surface to shoot growing point, cm), leaf area (3rd true leaf, cm2), plant fresh weight of whole plant (mg), and chlorophyll contents (SPAD value) at 6 weeks after silencing. Relative chlorophyll contents of the 3rd and 4th leaves of CaRLP-silenced plants were measured with a Chlorophyll Meter (Minolta Camera Co., Ltd., Japan). The silenced plants at 6 weeks after a VIGS assay were dried at 65 °C in an oven for two days, and then, dry weights of whole plants, shoot, and root were measured. All phenotypic analyses were performed using more than three independent experiments, with 8–10 plants for each experiment. Statistically significant differences (p < 0.05) of phenotypic analysis between control plants (TRV-GFP) and CaRLP-silenced plants (TRV-CaRLP) were determined using one-way ANOVA and Student’s t-test using SAS software (version 9.4; SAS Inc., Cary, NC, USA).
+ Open protocol
+ Expand
10

Cadmium Assessment in Plant Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
The plant tissue was harvested and dried for 3 h at 105°C and then for 3 days at 80°C. The dry weights of the samples were measured, then the samples were completely digested with extra pure grade HNO3/HClO4 (87/13, v/v). The ICP-AES 9800 was used to measure the levels of Cd (ICP-AES, inductively coupled plasma-atomic emission spectrometry). Fresh leaf chlorophyll content was measured by Chlorophyll Meter (SPAD-502, Konica Minolta, Japan) and expressed in SPAD units.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!