The morphological changes of each CaRLP-silenced pepper plant were measured, such as plant height (from soil surface to shoot growing point, cm), leaf area (3rd true leaf, cm
2), plant fresh weight of whole plant (mg), and chlorophyll contents (SPAD value) at 6 weeks after silencing. Relative chlorophyll contents of the 3rd and 4th leaves of CaRLP-silenced plants were measured with a
Chlorophyll Meter (Minolta Camera Co., Ltd., Japan). The silenced plants at 6 weeks after a VIGS assay were dried at 65 °C in an oven for two days, and then, dry weights of whole plants, shoot, and root were measured. All phenotypic analyses were performed using more than three independent experiments, with 8–10 plants for each experiment. Statistically significant differences (
p < 0.05) of phenotypic analysis between control plants (TRV-GFP) and CaRLP-silenced plants (TRV-CaRLP) were determined using one-way ANOVA and Student’s t-test using
SAS software (version 9.4; SAS Inc., Cary, NC, USA).
Kang W.H., Park B., Lee J, & Yeom S.I. (2021). Tissue-Specific RNA-Seq Analysis and Identification of Receptor-Like Proteins Related to Plant Growth in Capsicum annuum. Plants, 10(5), 972.