Bovine type Ⅱ collagen (CⅡ) was purchased from Chondrex (Redmond, WA, United States), Freund’s complete adjuvant (FCA) and Freund’s incomplete adjuvant (FIA) were purchased from Sigma (St.Louis, MO, United States). Lipid standards were purchased from Avanti Polar lipids, Inc. (Alabaster, AL, United States). Chromatographic grade methanol and chloroform were purchased from Merck (Darmstadt, Germany). Tripterygium wilfordii glycosides tablets were purchased from Deende Pharmaceutical Co., Ltd. (Zhejiang, China. Lot: 1206101, 10 mg/tablet). The ELISA kit for rat IL-1β was purchased from R&D systems (Minneapolis, MN, United States), and the anti-typeⅡ collagen antibodies (anti-CII) in serum were assayed by ELISA kit (Chondrex, Redmond, WA, United States).
Bovine type 2 collagen cii
Manufactured by Chondrex
Sourced in United States
Bovine type II collagen (CII) is a purified protein derived from bovine cartilage. It serves as a key component in various research applications focused on the study of cartilage and connective tissue function.
Automatically generated - may contain errors
Lab products found in correlation
Complete freund s adjuvant, by Chondrex (7 mentions)
Incomplete freund s adjuvant, by Chondrex (6 mentions)
Complete freund s adjuvant, by Merck Group (5 mentions)
Dba 1j mice, by Orient Bio (4 mentions)
Complete freund adjuvant (cfa), by Merck Group (3 mentions)
Complete freund s adjuvant cfa, by Chondrex (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Mycobacterium tuberculosis, by Chondrex (2 mentions)
Incomplete freund s adjuvant, by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Freund s incomplete adjuvant fia, by Merck Group (1 mentions)
Chromatographic grade methanol, by Merck Group (1 mentions)
Freund s complete adjuvant fca, by Merck Group (1 mentions)
Lipid standards, by Avanti Polar Lipids (1 mentions)
Chloroform, by Merck Group (1 mentions)
Elisa kit, by Chondrex (1 mentions)
Complete freund s adjuvant cfa, by Merck Group (1 mentions)
Dba 1j mice, by Central Lab Animal (1 mentions)
Cd4 t cell isolation kit 2, by Miltenyi Biotec (1 mentions)
3h thymidine, by MP Biomedicals (1 mentions)
41 protocols using bovine type 2 collagen cii
1
Experimental Induced Arthritis Model
2
Therapeutic Effects of T. spiralis and MES on CIA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male DBA/1 mice were used to generate a model of CIA following a previously described method (Cheng et al., 2018 (link)). Briefly, bovine type Ⅱ collagen (CⅡ) (Chondrex, Redmond, WA, USA) was dissolved in 0.01 M acetic acid at a concentration of 2 mg/mL by stirring overnight at 4°C and emulsified with an equal volume of complete Freund’s adjuvant (Chondrex). Male DBA/1 mice were intradermally immunized at the base of the tail with 0.1 mL of emulsion containing 100 μg of CⅡ followed by a booster comprising the same amount of CⅡ emulsified with incomplete Freund’s adjuvant on day 21. To evaluate the therapeutic effect of T. spiralis infection on CIA in mice, a total of 15 mice were divided into three groups (n = 5 mice per group), namely, an CIA group, in which CIA was induced by CⅡ immunization; a T. spiralis-infected group, in which mice were infected with 400 infective T. spiralis muscle larvae 14 days before the first CII immunization; and a control group, in which mice did not receive any treatment. To evaluate the therapeutic effect of MES on CIA in mice, a total of 15 mice were also divided into three groups (n = 5 mice per group), namely, an CIA group; an intraperitoneal injection of protein group, in which mice were treated via the intraperitoneally injection route with 20 μg/mice of MES on day −2, 0, 21 and 28 of the first CII immunization; and a control group.
3
Collagen-Induced Arthritis Model in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CIA model was established according to reference [26 (link)–28 (link)]. Briefly, bovine type II collagen (CII; Chondrex, Redmond, WA, USA) (dissolved in 0.1 M acetic acid) was emulsified 1 : 1 (v: v) with complete Freund's adjuvant (CFA; Sigma-Aldrich, Darmstadt, Germany) at a final concentration of 1 mg/mL. Rats were immunized with intradermal injection of the 300 μl CII emulsion at the base of the tail on day 0, followed by the same booster injection 7 days later. The animals with no signs of swelling in joints were removed. Controls were handled in the same way except that they were injected with 150 μl of 0.1 M acetic acid.
4
Collagen-Induced Arthritis in DBA/1 Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experimental arthritis was induced in DBA/1 mice based on the method previously described (31 (link)). Bovine type II collagen (CII) purchased from Chondrex (Redmond, WA, USA) was dissolved in 0.01 M acetic acid at concentration of 2 mg/ml by stirring over night at 4°C and emulsified with the equal volume of complete Freund’s adjuvant. Male DBA/1 mice were immunized intradermally at the base of the tail with 0.1 ml emulsion containing 100 μg CII. The mice were boosted once with the same amount of CII emulsified with incomplete Freund’s adjuvant (Chondrex) 21 days after the first immunization. Induced arthritic mice were clinically assessed for redness and swelling of all limbs every other day up to 50 days. The clinical scores were assigned as previously described to evaluate disease (32 (link)) as follows: 0 = no signs of arthritis: 1 = swelling and/or redness of the paw or one digit; 2 = two joints involved; 3 = more than two joints involved and 4 = severe arthritis of the entire paw and digits. Each limb was graded, resulting in a maximal clinical score of 16 per animal.
5
Collagen-Induced Arthritis Induction in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male 6-week old DBA/1J mice (Central Lab. Animal Inc., Seoul, Korea) were used to induce CIA. Bovine type II collagen (CII, 2 mg/ml; Chondrex, Redmond, WA, USA) was diluted in an equal volume of complete Freund's adjuvant (Chondrex). Mice were first immunized with 50 μl emulsion containing 50 μg CII via intradermal (i.d.) injection into the tail. After 14 days, a booster injection of 50 μl of an emulsion of CII in incomplete Freund's adjuvant (Chondrex) was administered through i.d. injection at the same site in the tail. After booster, Erdr1 (100 μg/kg) or phosphate-buffered saline (PBS) (vehicle control) was administered by intraperitoneal (i.p.) injection three times/week. All experimental procedures involving mice were approved by the Institutional Animal Care and Use Committee of Sookmyung Women's University.
6
Suppressive Function of Foxp3-Transfected B Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CD4+CD25− T cells were isolated from spleens of arthritic mice by magnetic bead cell sorting using an untouched CD4+ T Cell Isolation Kit II and CD25 Microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. To assess the suppressive activities of Foxp3-transfected CD19+ B cells, CD4+CD25−responder T cells (5 × 104/well) were cultured with a 1:1 ratio of shRNA or Foxp3-transfected CD19+ B cells (which were stimulated with LPS or anti-IgM) in the presence or absence of bovine type II collagen (CII) (Chondrex Inc., Redmond, WA, USA), in a 100 ng/ml anti-CD3-coated 96-well plate. In some cases, Foxp3-transfected CD19+ B cells were placed in the inner transwell chamber. During the last 16 h of culture, cells were pulsed with 3H-thymidine (1 μCi/well; MP Biomedicals, Seven Hills, Australia). Cells were assessed for thymidine incorporation in a Microbeta counter (Wallac Oy 1450 MicroBeta; Wallac, Melbourne, Australia).
7
Collagen-Induced Arthritis Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IL-10−/− DBA/1J and Wild type mice were inoculated intradermally on day 0 and 21 with 200 μl bovine type II collagen (CII; Chondrex) emulsified in CFA containing Mycobacterium tuberculosis (Chondrex). For the lentivirus-IL-10 treatment, A total of 107 TU/ml lentivirus containing the mouse IL-10 gene (LV-IL-10) or empty lentivirus control (LV-empty) (GeneChem, Shanghai, China) was injected intra-articularly into the knee joints on day 5 and 18 after the first CII immunization. For details of the lentivirus see http://www.genechem.com.cn . Samples were harvested from LV-IL-10 and LV-empty treated CIA mice on day 35 after the first immunization. For neutralizing IL-33 experiments, 100 μg anti-IL-33 antibodies (R&D Systems, USA) were given intraperitoneally on day 14 and followed with same dose every four days apart. Mice were sacrificed on day 41, and samples were isolated for performing experimental analysis.
8
Collagen-Induced Arthritis Model in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For CIA induction, bovine type II collagen (CII; Chondrex, Redmond, WA) was emulsi ed with complete Freund's adjuvant (CFA) containing 4 mg/ml heat-denatured mycobacterium (Chondrex, LLC, Seattle, WA) at a ratio of 1:1 (100 ug/mouse) and then injected intradermally into the tail (1.5 cm from the base) of DBA1/J mice to induce CIA, followed by a booster immunization with 100 ug CII in incomplete adjuvant (IFA, Chondrex, Redmond, WA) [33] . At the moment of the boost (day 21), GMSC (1×10 6 /mouse) and GMSC-Exo (165 ug/mouse) were injected into CIA mice (n = 6) via the lateral tail vein. In the control group, mice received equal PBS injection (n = 6).
Mice were monitored twice weekly for signs of arthritis based on arthritis scores and paw swelling. Each paw was evaluated and scored individually using a 0 to 4 scoring system: 0 = no damage; 1 = paw with detectable swelling in a single digit; 2 = paw with swelling in more than one digit; 3 = paw with swelling of all digits and instep; and 4 = severe swelling of the paw and ankle [34] . At the day 56, all mice were euthanized and peripheral blood, spleen, lymph nodes (LN), and limbs were collected for further studies.
Mice were monitored twice weekly for signs of arthritis based on arthritis scores and paw swelling. Each paw was evaluated and scored individually using a 0 to 4 scoring system: 0 = no damage; 1 = paw with detectable swelling in a single digit; 2 = paw with swelling in more than one digit; 3 = paw with swelling of all digits and instep; and 4 = severe swelling of the paw and ankle [34] . At the day 56, all mice were euthanized and peripheral blood, spleen, lymph nodes (LN), and limbs were collected for further studies.
9
Collagen-Induced Arthritis in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 18 inbred female rats (Sprague-Dawley; age, 7–8 weeks) weighing ≥150 g were purchased from Shanghai SLAC Laboratory Animal Co., Ltd., (Shanghai, China) and were allowed to acclimate for ≥1 week prior to experimentation. The rats were housed in an animal facility and were maintained at 25°C (humidity, 60–70%) under a 12 h light/dark cycle with ad libitum access to food and water. Bovine type II collagen (CII; Chondrex, Inc., Redmond, WA, USA) was used to induce RA, which was dissolved in 0.05 mol/l acetic acid to a final concentration of 2.0 mg/ml, and was vortexed overnight at 4°C. Subsequently, CII was emulsified with complete Freund's adjuvant (Chondrex, Inc.) at a ratio of 1:1. The rats were immunized with 300 µl CII emulsion by subcutaneous injection into the tail root. On day 7, the rats received a subcutaneous booster injection (300 µl) into the tail; the primary injection site was avoided. After 28 days following the induction of the RA model via CII, the rats were sacrificed. All procedures that involved animals were performed in accordance with the institutional animal welfare guidelines of Tongji University (14 (link)). The rats were divided into the following groups: i) Control group, in which RA was not induced (n=6) and were treated with saline and an ii) RA group, in which RA was induced via CII (n=12). Rats were examined three times per week.
10
Induction and Treatment of Collagen-Induced Arthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bovine type II collagen (CII; Chondrex, Redmond, WA, USA) was emulsified with an equal volume of complete Freund’s adjuvant (Sigma-Aldrich, St. Louis, MO, USA). DBA/1J mice were immunized via intradermal injection at the base of the tail with 100 μL of emulsion on day 0 as the first immunization. On day 21 after immunization, a booster with 100 μL of the same bovine CII emulsified in incomplete Freund’s adjuvant (Chondrex, Redmond, WA, USA) was administered by the same route.
For the oral administration study, mice were gavaged daily with kaempferol (purity: 98%; MUST, Chengdu, China) at a dose of 200 mg/kg or leflunomide (purity: 98%) at a dose of 5 mg/kg from day 21 to day 48. For the alternative intraperitoneal injection study, 20 mg/kg of kaempferol was injected daily from day 21 to day 48.
For the oral administration study, mice were gavaged daily with kaempferol (purity: 98%; MUST, Chengdu, China) at a dose of 200 mg/kg or leflunomide (purity: 98%) at a dose of 5 mg/kg from day 21 to day 48. For the alternative intraperitoneal injection study, 20 mg/kg of kaempferol was injected daily from day 21 to day 48.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!