I1 mouse hybridoma

Manufactured by American Type Culture Collection

The I1 mouse hybridoma is a cell line produced by fusing mouse myeloma cells with spleen cells from a mouse immunized with a specific antigen. This hybridoma can be used to produce monoclonal antibodies targeting the specific antigen of interest.

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Lab products found in correlation

Vero e6 cells, by Thermo Fisher Scientific (2 mentions) Freestyle 293 f, by Thermo Fisher Scientific (2 mentions) Freestyle 293 expression medium, by Thermo Fisher Scientific (2 mentions) Vero e6 cell, by American Type Culture Collection (2 mentions) Freestyle 293 f cells, by Thermo Fisher Scientific (2 mentions) Expi293f cells, by Thermo Fisher Scientific (2 mentions) Expi293 expression medium, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions) Hek293t 17, by American Type Culture Collection (2 mentions) Hek293s gnti, by American Type Culture Collection (2 mentions) Hek293t 17, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

I1-Hybridoma (4 citations) I1-mouse hybridoma supernatant (4 citations) I1 mouse hybridoma cells (2 citations) Anti-VSV-G antibody (I1-mouse hybridoma supernatant (2 citations)

2 protocols using i1 mouse hybridoma

Cell Culture Protocols for Diverse Cell Lines

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FreeStyle 293-F (cat# R79007), Expi293F cells (cat# A14635) were from Thermo Fisher Scientific. HEK293S GnTI- (cat# CRL-3022), HEK293T/17 (cat# CRL-11268), I1 mouse hybridoma (cat# CRL-2700) and Vero E6 cells (cat# CRL-1586) were from ATCC.
FreeStyle 293-F cells and were cultured in serum-free FreeStyle 293 Expression Medium (GIBCO, cat# 12338026) at 37°C, 10% CO₂, 115 rpm. HEK293S GnTI- cells were cultured in FreeStyle 293 Expression Medium at 37°C, 10% CO₂, 115 rpm. Expi293F cells were cultured in Expi293 Expression Medium (GIBCO, cat# A14635) at 37°C, 8% CO₂, 125 rpm. HEK293T/17 cells and Vero E6 cells were cultured in 10% Fetal Bovine Serum (FBS, GIBCO cat# 16140071) supplemented Dulbecco’s Modified Eagle Medium (DMEM, ATCC cat# 30-2002) at 37°C, 5% CO₂. Cell lines were not specifically authenticated.

Cerutti G., Guo Y., Zhou T., Gorman J., Lee M., Rapp M., Reddem E.R., Yu J., Bahna F., Bimela J., Huang Y., Katsamba P.S., Liu L., Nair M.S., Rawi R., Olia A.S., Wang P., Zhang B., Chuang G.Y., Ho D.D., Sheng Z., Kwong P.D, & Shapiro L. (2021). Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite. Cell Host & Microbe, 29(5), 819-833.e7.

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Cell Culture Protocols for Diverse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

FreeStyle 293-F (cat# R79007), Expi293F cells (cat# A14635) were from Thermo Fisher Scientific. HEK293S GnTI- (cat# CRL-3022), HEK 293T/17 (cat# CRL-11268), I1 mouse hybridoma (cat# CRL-2700) and Vero E6 cells (cat# CRL-1586) were from ATCC. FreeStyle 293-F cells and were cultured in serum-free FreeStyle 293 Expression Medium (Gibco, cat# 12338026) at 37 °C, 10% CO₂, 115 rpm. HEK 293S GnTI- cells were cultured in FreeStyle 293 Expression Medium at 37 °C, 10% CO₂, 115 rpm. Expi293F cells were cultured in Expi293 Expression Medium (Gibco, cat# A14635) at 37 °C, 8% CO₂, 125 rpm. HEK 293T/17 cells and Vero E6 cells were cultured in 10% Fetal Bovine Serum (FBS, Gibco cat# 16140071) supplemented Dulbecco’s Modified Eagle Medium (DMEM, ATCC cat# 30–2002) at 37 °C, 5% CO₂. Cell lines were not specifically authenticated.

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