Purified antibodies were filtered using Cosmo spin filter H (Nacalai Tesque, Inc.) to remove precipitates or debris before SEC analysis. Then, the antibodies were passed through an Agilent Bio SEC-5 500 Å (7.8×300 mm) column (Agilent Technologies) coupled to an Agilent 1260 Infinity Bio-inert HPLC system (Agilent Technologies). Analyses were performed using 1 μg or more of each antibody sample, with a flow rate of 1 ml/min. PBS (pH 7.4) was used as an eluent. Data were analyzed using OpenLAB CDS ChemStation Edition (Agilent Technologies).
Agilent 1260 infinity bio inert hplc system
Manufactured by Agilent Technologies
Sourced in Germany
The Agilent 1260 Infinity Bio-inert HPLC system is a high-performance liquid chromatography (HPLC) instrument designed for bioanalytical applications. The system features a bio-inert flow path, ensuring compatibility with sensitive biomolecules and preventing analyte adsorption or degradation. The core function of the Agilent 1260 Infinity Bio-inert HPLC system is to separate, identify, and quantify various biomolecules, such as proteins, peptides, and other biological compounds, within a sample.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 8800 icp ms ms instrument, by Agilent Technologies (3 mentions)
Openlab cds chemstation edition, by Agilent Technologies (1 mentions)
Cosmospin filter h, by Nacalai Tesque (1 mentions)
Agilent 8800 icp ms ms, by Agilent Technologies (1 mentions)
Atlantis t3 guard column, by Waters Corporation (1 mentions)
Atlantis t3 c18 analytical column, by Waters Corporation (1 mentions)
6 protocols using agilent 1260 infinity bio inert hplc system
1
Antibody Purification and Characterization via SEC
2
Centrifugal Ultrafiltration of Platinum Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Centrifugal ultrafiltration was carried out using Amicon Ultra 0.5-mL centrifugal regenerated cellulose membrane filters, with a 10-kDa molecular weight cut-off (MWCO) (Millipore Co, Carrigtwohill, Co. Cork, Ireland). The samples were ultracentrifuged with a Hermle Z 466 K device with a precooled rotor (4 °C) at a G-force of 12,500g. For preconditioning of the membrane filters and ultracentrifugation of the drug solutions, a centrifugation time of 60 min and 15 min was used, respectively. The filters used for centrifugal ultrafiltration were preconditioned using the solvents with the highest recovery for the drugs, respectively. For KP2156, 50 mM CH3COONH4, pH = 6.0 was employed, whereas for KP2157 FCS was used. Flow injection measurements for platinum quantification were performed using an Agilent 1260 Infinity Bio-inert HPLC system (Agilent Technologies, Waldbronn, Germany) coupled to an Agilent 8800 ICP–MS/MS (Agilent Technologies, Tokyo, Japan). The HPLC system was operated with CH3COONH4 (50 mM, pH = 6) as eluent, a flow rate of 250 μL/min, and an injection volume of 10 μL. The ICP–MS operation parameters are summarized in Table 1 .
3
Quantitative Protein Analysis by UHPLC-SEC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Agilent 1260 infinity Bio-inert HPLC system (Agilent Technologies, Waldbronn, Germany) was employed for the UHPLC SEC measurements. For the quantification studies with conventional SEC, the Thermo Scientific Transcend HPLC system (San Jose, CA, USA) was used. The chromatographic conditions are given in Table 2 .
Chromatographic conditions
| Instrumentation HPLC | Agilent 1260 infinity Bio-inert | Thermo Scientific Transcend system |
|---|---|---|
| HPLC Column | Acquity UPLC Protein BEH SEC, 4.6 × 150 mm, 125A, 1.7 μm, 1 kDa–80 kDa, Waters | BioBasic SEC-60 A, 4.6 × 250 mm, 5 μm, 0.1 kDa–10,000 kDa Thermo |
| Eluent | 50 mM CH3COONH4, pH = 6.0 | 50 mM CH3COONH4, pH = 6.0 |
| Flow rate | 400 μL/min | 250 μL/min |
| Injection volume | 5 μL | 10 μL |
| Column temperature | 30 °C | Room temperature |
| Autosampler Temperature | 4 °C | 4 °C |
| Combined with | ICP–MS/MS Agilent 8800 | ICP-QMS iCAP Thermo Scientific |
4
ICP-MS/MS Method for Elemental Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Agilent 8800 ICP-MS/MS instrument (Agilent Technologies, Tokyo, Japan) with oxygen as reaction gas was used to determine the content of the respective elements. The ICP-MS parameters were tuned on a daily based to achieve high sensitivity. The ICP-MS was equipped with a MicroMist nebulizer with a sample uptake rate of ∼0.25 ml·min−1 and standard nickel cones. The instrument was coupled to an Agilent 1260 Infinity Bio-Inert HPLC system (Agilent Technologies, Waldbronn, Germany). The Agilent MassHunter software package (Workstation Software, Version C.01.06, 2019) was used for data evaluation. The instrumental parameters for the ICP-MS measurements are summarized in Table 2 .
5
Sulfur Determination by ICP-MS/MS with Oxygen Reaction Gas
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Agilent 8800 ICP-MS/MS instrument (Agilent Technologies, Tokyo, Japan) with oxygen as reaction gas was used to determine the sulfur content. The ICP-MS parameters were tuned on a daily base to achieve high sensitivity. The ICP-MS was equipped with a MicroMist nebulizer with a sample uptake rate of ∼ 0.25 mL min−1 and standard nickel cones. All sulfur isotopes were measured using oxygen as reaction gas (flow rate = 0.32 mL min−1) with mass selection steps on both quadrupoles. The following isotopes were monitored with a respective integration time of 0.1 s: 32S16O+, 33S16O+, 34S16O+, and 36S16O+. The instrument was coupled to an Agilent 1260 Infinity Bio-Inert HPLC system (Agilent Technologies, Waldbronn, Germany). The Agilent MassHunter software package (Workstation Software, Version C.01.03, 2016) was used for data evaluation. The instrumental parameters for the ICP-MS measurements are summarized in Table 1 .
Instrumental parameters for the ICP-MS/MS measurements
| RF power [W] | 1550 |
|---|---|
| Nebulizer | MicroMist |
| Spray chamber | Scott double-pass |
| Spray chamber temp. [°C] | 2 |
| Cone materials | Ni |
| Plasma gas flow (Ar) [L min−1] | 15.0 |
| Auxiliary gas flow (Ar) [L min−1] | 1.10 |
| Nebulizer gas flow (Ar) [L min−1] | 0.90 |
| Reaction gas flow (O2) [mL min−1] | 0.32 |
| Monitored isotopes | 32S16O, 33S16O, 34S16O, 36S16O |
| Integration time | 0.1 s |
6
Quantifying Trace Elements via RP-ICP-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RP-ICP-MS measurements were performed on an Agilent 1260 Infinity Bio-inert HPLC system (Agilent Technologies, Waldbronn, Germany) hyphenated to an Agilent 8800 ICP-MS/MS instrument (Agilent Technologies, Tokyo, Japan). Chromatographic separations were performed using an Atlantis T3 C18 analytical column (150 mm L × 2.1 mm i.d., 3 μm dp, 100 Å pore size) equipped with an Atlantis T3 guard column (20 mm L × 2.1 mm i.d., 3 μm dp) from Waters (Milford, USA). Chromatographic conditions are summarized in Table S2.† The ICP-MS/MS was equipped with a torch with 1.5 mm inner diameter, platinum cones and the brass-based lens system in order to withstand the high organic solvent load. Oxygen was introduced as the option gas and admixed with the aerosol before entering the ICP-MS to avoid carbon deposition on the cones. Data processing was performed with the Agilent MassHunter software package (Workstation Software, Version C.01.03, 2016). The ICP-MS operation parameters and chromatographic conditions used are summarized in Tables S1 and S2,† respectively. Samples were measured in triplicates and an aqueous solution of complex 3 (50 μM) was measured after every third sample as quality control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!