Cd24 apc

Manufactured by Miltenyi Biotec

Sourced in Australia, Germany

CD24-APC is a fluorescently labeled antibody that binds to the CD24 cell surface antigen. CD24 is a glycosylphosphatidylinositol (GPI)-linked cell surface protein expressed on various cell types. The APC (Allophycocyanin) fluorescent dye is conjugated to the CD24 antibody, allowing for the detection and analysis of CD24-positive cells using flow cytometry.

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Lab products found in correlation

Cd44 pe, by Miltenyi Biotec (3 mentions) Mouse e cadherin, by BD (1 mentions) Sca1 pe, by Miltenyi Biotec (1 mentions) Epcam apc, by Thermo Fisher Scientific (1 mentions) Ep17 ep30, by Agilent Technologies (1 mentions) Ab5694, by Abcam (1 mentions) Facscanto 2, by BD (1 mentions) Cd133 apc, by Miltenyi Biotec (1 mentions) Aldefluor assay, by STEMCELL (1 mentions) Attune nxt, by Thermo Fisher Scientific (1 mentions) Cd133 fitc, by Miltenyi Biotec (1 mentions) Cd105 apc, by Miltenyi Biotec (1 mentions) Cd90 fitc, by Miltenyi Biotec (1 mentions) Cd146 pe, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

Anti-CD24 (APC) (3 citations)

3 protocols using cd24 apc

Comprehensive Antibody Panel for Cellular Characterization

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The antibodies used for Western blotting (WB), immunofluorescence (IF), immunohistochemistry (IHC) and flow cytometry (FC) were as follows: human and mouse Annexin A1 (WB, IF), mouse mAb clone EH17a (Santa Cruz Biotechnology, Dallas, TX, USA). Human Annexin A1 (IHC), mouse mAb clone MRQ-3 (Cell Marque, Sigma Aldrich). Mouse E-cadherin (IF, IHC), mouse mAb clone E-cad/36 (BD Transduction Laboratories, BD Biosciences, Scoresby, Vic., Australia). Mouse CD24 (FACS), CD24-PE-Vio770 or CD24-APC, rat mAb clone M1/69 (Miltenyi Biotec, Macquarie Park, NSW, Australia), mouse CD44 (FACS), CD44-PE, rat mAb clone IM7.8.1 (Miltenyi Biotec), mouse Epcam/CD326 (FACS), Epcam-APC, rat mAb clone G8.8 (eBioscience, Invitrogen, Thermo Fisher Scientific, Scoresby, Vic., Australia). Mouse cytokeratin 14 (IHC), mouse mAb clone LL002 (Abcam, Melbourne, Vic, Australia). Mouse pan-cytokeratin (recognizing type II cytokeratins 1, 5, 6, and 8) (IHC), mouse mAb clone PCK-26 (Sigma-Aldrich). Mouse cytokeratins 8–18 (IHC), rabbit mAb clones EP17/EP30 (Dako, Leica Microsystems, Macquarie Park, NSW, Australia). Mouse alpha smooth muscle actin (αSma) (IF), rabbit pAb ab5694 (Abcam). Mouse Sca1 (Ly6a) (FACS), Sca1-PE, rat mAb clone D7 (Miltenyi Biotec). Mouse vimentin (IHC), rabbit pAb R28 (Cell Signaling Technology, Danvers, MA, USA).

Johnstone C.N., Tu Y., Langenbach S., Baloyan D., Pattison A.D., Lock P., Britt K.L., Lehmann B.D., Beilharz T.H., Ernst M., Anderson R.L, & Stewart A.G. (2021). Annexin A1 Is Required for Efficient Tumor Initiation and Cancer Stem Cell Maintenance in a Model of Human Breast Cancer. Cancers, 13(5), 1154.

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Aldehyde Dehydrogenase Activity Profiling

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ALDEFLUOR assays (Stem Cell Technologies) to detect ALDH1 activity were performed according to manufacturer’s instructions. Diethylaminobenzaldehyde (DEAB) was used as an ALDH1 inhibitor to set ALDH1 gates. Cell surface levels were determined with anti-human antibodies CD44-PE, CD24-APC, CD133-APC, CD326-FITC, CK18-FITC and CK20-FITC (Miltenyi Biotec). All samples were analyzed on a FACS CANTO II (BD Biosciences) using the FACS DIVA software.

Morata-Tarifa C., Jiménez G., García M.A., Entrena J.M., Griñán-Lisón C., Aguilera M., Picon-Ruiz M, & Marchal J.A. (2016). Low adherent cancer cell subpopulations are enriched in tumorigenic and metastatic epithelial-to-mesenchymal transition-induced cancer stem-like cells. Scientific Reports, 6, 18772.

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Flow Cytometry Immunophenotyping of Cells

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For flow cytometry analysis, the cells were resuspended from the pellet in PBS from each sample and divided into different tubes. Each tube was individually incubated for 30 min at 4 °C with ECAD-APC, NCAD-PE, CD90-FITC, CD105-APC, CD24-APC, CD44-PE, CD133-FITC, and CD146-PE antibodies (all monoclonal) (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation, PBS was used to wash the cells and pelleted down. The labelled cells were resuspended in sheath fluid and analyzed with a flow cytometer (Attune NxT, Thermo Fisher Scientific, Waltham, MA, USA). At least for each samples 10,000 events are acquired. Percentage comparison was done with the degree of positive staining and the isotype controls. The median fluorescence intensities for each surface protein were also considered.

Baeshen H.A., Alsulaimani F.F., Awadh W., Ageeli M.A., Abullais S.S., Alqahtani N.A., Alshahrani M.Y., Hosmani J, & Patil S. (2021). Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances. Saudi Journal of Biological Sciences, 29(3), 1789-1795.

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