Log In Sign up
The largest database of trusted experimental protocols

Mouse total tau assay

Manufactured by Quanterix
Visit Supplier

The Mouse 'total-tau' assay by Quanterix is a laboratory instrument designed to measure the levels of total tau protein in mouse samples. The assay provides a quantitative analysis of the target analyte without making claims about its intended use or interpretation of the results.

Automatically generated - may contain errors

Lab products found in correlation

Hd x analyzer, by Quanterix (3 mentions) Nf light advantage kit, by Quanterix (1 mentions) Sr x analyzer, by Quanterix (1 mentions) Hd 1 analyzer, by Quanterix (1 mentions)

4 protocols using mouse total tau assay

1

Quantitative Antemortem Tau Biomarker

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antemortem CSF tau was quantified by Simoa technology using the mouse ‘total-tau assay (102209, Quanterix), as described in (Simoes et al., 2020 (link)). Briefly, individual CSF samples (4uL) were diluted 1:60 in sample buffer and then split into technical duplicates. Standards and internal controls were run according to the manufacturer’s instructions and read on the HD-X analyzer (Quanterix).
Simoes S., Guo J., Buitrago L., Qureshi Y.H., Feng X., Kothiya M., Cortes E., Patel V., Kannan S., Kim Y.H., Chang K.T., Hussaini S.A., Moreno H., Di Paolo G., Andersen O.M, & Small S.A. (2021). Alzheimer’s vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling. Cell reports, 37(13), 110182.
+ Open protocol
+ Expand
2

Quantitative Antemortem Tau Biomarker

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antemortem CSF tau was quantified by Simoa technology using the mouse ‘total-tau assay (102209, Quanterix), as described in (Simoes et al., 2020 (link)). Briefly, individual CSF samples (4uL) were diluted 1:60 in sample buffer and then split into technical duplicates. Standards and internal controls were run according to the manufacturer’s instructions and read on the HD-X analyzer (Quanterix).
Simoes S., Guo J., Buitrago L., Qureshi Y.H., Feng X., Kothiya M., Cortes E., Patel V., Kannan S., Kim Y.H., Chang K.T., Hussaini S.A., Moreno H., Di Paolo G., Andersen O.M, & Small S.A. (2021). Alzheimer’s vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling. Cell reports, 37(13), 110182.
+ Open protocol
+ Expand
3

Quantification of NEFL and Total-Tau

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse plasma and CSF NEFL concentration was quantified using commercially available NF-light™ Advantage Kit (103186, Quanterix). Total-Tau concentrations were quantified using the mouse total-Tau assay (102209, Quanterix) which detects the mid-protein epitope of the Tau protein. Plasma samples were diluted 1:40 and CSF samples 1:100 in the sample buffer provided in the kits as per manufacturer instructions. Standards and samples were run in technical duplicates for single-molecule array (SIMOA) immunoassay and run on the HD-X analyzer using the two-step assay as per manufacturer’s instructions (Quanterix). For NEFL, the lower limit of quantification (LLOQ) was 0.174 pg/mL, and the limit of detection (LOD) was 0.038 pg/mL. For total-Tau, the LLOQ was 0.823 pg/mL, and the limit of detection was 0.428 pg/mL. The intra-assay coefficients of variability of replicates were automatically calculated by the instrument to assess the repeatability of the test and expressed as percentages.
Bondulich M.K., Phillips J., Cañibano-Pico M., Nita I.M., Byrne L.M., Wild E.J, & Bates G.P. (2024). Translatable plasma and CSF biomarkers for use in mouse models of Huntington’s disease. Brain Communications, 6(1), fcae030.
+ Open protocol
+ Expand
4

Quantifying Tau Protein in Mouse CSF

Check if the same lab product or an alternative is used in the 5 most similar protocols
In Fig. 4B (left), postmortem CSF tau was quantified by Simoa Technology using the mouse “total-tau assay” (102209, Quanterix), which is directed against the mid-protein epitope of the tau holo-protein. Individual CSF samples (5 μl) were diluted 1:80 in sample buffer and then split into technical duplicates. Standards and samples were run according to the manufacturer’s instructions and read on the HD-1 analyzer (Quanterix). Replication of CSF tau elevation in 3- and 6-month-old mice shown in Fig. 4B (right) was performed using antemortem CSF samples collected from two different cohorts (22 females and 20 males) of anesthetized animals in accordance with Columbia University IACUC guidelines. For the latter, individual CSF samples (2 μl) were diluted 1:100 in sample buffer and then split into technical duplicates and read on the SR-X analyzer (Quanterix). CSF samples from Tau KO mice were used as negative controls.
Simoes S., Neufeld J.L., Triana-Baltzer G., Moughadam S., Chen E.I., Kothiya M., Qureshi Y.H., Patel V., Honig L.S., Kolb H, & Small S.A. (2020). Tau and other proteins found in Alzheimer’s disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans. Science translational medicine, 12(571), eaba6334.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!