Ldh release

Manufactured by Roche

The LDH release product is a laboratory equipment designed to measure the activity of the enzyme lactate dehydrogenase (LDH) in biological samples. LDH is an important marker for various medical conditions, and its release can indicate cellular damage or death. This product provides a quantitative assessment of LDH levels, enabling researchers and clinicians to obtain relevant data for their scientific or diagnostic purposes.

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Lab products found in correlation

Annexin 5, by BD (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions)

Close spelling variants of this product

LDH release kit LDH release detection kit LDH) release assay Lactate dehydrogenase (LDH) release detection kit Lactate dehydrogenase (LDH) release assay Cytotoxicity detection kit (LDH release assay) LDH Release assay kit

2 protocols using ldh release

Monitoring Cell Death Mechanisms

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Cell death was monitored by LDH release (Roche) following the manufacturer's instructions. Briefly, cells were treated for 24 h with 0.5 µM reversine or 2 µM AZ3146. Cells were then washed with PBS, and LDH release was measured 48 h later. Staurosporine (1 µM) was used as a positive control. Annexin V (BD Pharmigen) detection was performed as described previously (Tang et al. 2011 (link)).

Santaguida S., Vasile E., White E, & Amon A. (2015). Aneuploidy-induced cellular stresses limit autophagic degradation. Genes & Development, 29(19), 2010-2021.

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Screening PTK Inhibitors for Inflammasome Modulation

Cited 1 time

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For the screening of the PTK library (Selleckchem, Houston, TX) we used THP-1 cells and a 96-well plate format. All inhibitors were dissolved in DMSO and used in screening at 3 concentrations: 0.1 μM, 1 μM and 10 μM. Cells were pretreated with inhibitors for 30 min and then stimulated with 1 μg/ml of LPS (InvivoGen, San Diego, CA) for 30 min followed by 5 mM of ATP (Sigma-Aldrich) for 30 minutes. In some experiments, to further evaluate selected inhibitors, cells were infected with 100 MOI of F. novicida or 10 MOI of B. cenocepacia for 6 h. Cell culture media was collected and centrifuged for 5 minutes at 300 g. Cell death was determined by LDH release (Roche Applied Science) and inflammasome-dependent IL-18 release by ELISA, as we described earlier [8 (link)]. DMSO pre-treated cells served as a control and the AG126 PTK inhibitor (InvivoGen, San Diego, CA) served as a positive control for inflammasome inhibition [21 (link)]. To evaluate the effect of PTK inhibitors, a heat map was generated with fold difference changes related to DMSO control (100% or 1).

Gavrilin M.A., Prather E.R., Vompe A.D., McAndrew C.C, & Wewers M.D. (2021). cAbl kinase regulates inflammasome activation and pyroptosis via ASC phosphorylation. Journal of immunology (Baltimore, Md. : 1950), 206(6), 1329-1336.

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