Aldo, Rapamycin (Rap), and Chloroquine (CQ) were obtained from Sigma Aldrich (St. Louis, MO). Anti-LC3 (4108) and anti-GAPDH (8884) antibodies were purchased from Cell Signaling Technology (Beverly, MA). Anti-p62 (ab109012), Anti-p21 (ab109199), anti-NOX2 (ab129068) and anti-NOX4 (ab109225) antibodies were purchased from Abcam (Cambridge, MA). Anti-β-actin (AB2001) was purchased from Abways. ROS/RNS Assay Kit (ab238535) was purchased from Abcam (Cambridge, MA).
Anti gapdh 8884
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-GAPDH (8884) is a primary antibody that recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-established housekeeping protein used as a loading control in Western blotting experiments. This antibody is suitable for the detection of GAPDH across a wide range of species.
Automatically generated - may contain errors
Lab products found in correlation
Chloroquine cq, by Merck Group (1 mentions)
Anti lc3 4108, by Cell Signaling Technology (1 mentions)
Rapamycin rap, by Merck Group (1 mentions)
Mannitol, by Merck Group (1 mentions)
Anti chop, by Cell Signaling Technology (1 mentions)
Anti bip, by Cell Signaling Technology (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti p enos, by Cell Signaling Technology (1 mentions)
Anti t jnk, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 1, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Kangchen Biotech (1 mentions)
Sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page, by Merck Group (1 mentions)
Anti p jnk 4668, by Cell Signaling Technology (1 mentions)
Anti gp91phox, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Kangchen Biotech (1 mentions)
Anti t akt, by Cell Signaling Technology (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
3 protocols using anti gapdh 8884
1
Molecular Pathways Regulation via Rap and CQ
2
Hydrogen Sulfide Signaling in Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium hydrosulfide (NaHS; a donor of H2S) was purchased from Gibco-BRL (Grand Island, NY, USA). Fetal bovine serum (FBS), 2′, 7′-dichlorofluorescein diacetate (DCFH-DA), 740 Y-P (a PI3K agonist), LY294002 (a reversible PI3K inhibitor), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), M200 medium, D-glucose, Hoechst 33258 and mannitol were supplied by Sigma-Aldrich (St Louis, MO, USA). Cell counter kit-8 (CCK-8) was purchased from Dojindo Lab (Kumamoto, Japan). Anti-GAPDH (#8884), anti-ATF6 (#65880), anti-CHOP (#2895), anti-BiP (#3177), anti-phospho (p)-PI3K (#4228), anti-p-Akt (#4060), anti-p-eNOS (#9570), anti-total (t)-PI3K (#4249), anti-t-Akt (#4685), anti-t-eNOS (#9586), anti-Bax (#5023), anti-Bcl2 (#2827), anti-cleaved caspase 3 (#9661), anti-cleaved caspase 1 (#4199), anti-p-JNK(#4668), anti-t-JNK (#9252) and anti-gp91phox (#80897) antibodies were from Cell Signaling Technology (Boston, MA, USA). Enhanced chemiluminescence (ECL) solution was purchased from KeyGen Biotech (Nanjing, China). Interleukin (IL)-1β (#ab46052), IL-6 (#ab46027) and tumor necrosis factor (TNF)-α (#ab10054) ELISA kits were provided by Abcam (Cambridge, UK). Horseradish peroxidase (HRP)-conjugated secondary antibody and BCA protein assay kit were obtained from KangChen Bio-tech, Inc (Shanghai, China).
3
Mechanistic Study of Metabolic Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
6-Aminonicotinamide (6-AN) and N-Acetyl-L -cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Cis-diamminedichloroplatinum (II) (cisplatin, CDDP) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Dimethyl sulfoxide (DMSO) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from MP Biomedicals (Santa Ana, CA, United States). Rabbit polyclonal anti-G6PD (8866), anti-PGD (13389), anti-TKT (8616) and anti-GAPDH (8884) were purchased from Cell Signaling Technology (Danvers, MA, United States), Rabbit polyclonal anti-TALDO1 (D123398) was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Other chemicals were of analytical grade from commercial suppliers.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!