Ab24175

Manufactured by Abcam

Ab24175 is a lab equipment product offered by Abcam. It serves as a core functional component for scientific research and experiments. The detailed specifications and intended use of this product are not available for an unbiased and factual description.

Automatically generated - may contain errors

Lab products found in correlation

Ab4086, by Thermo Fisher Scientific (2 mentions) Ab31972, by Abcam (2 mentions) Ab24174, by Abcam (2 mentions) Ab1791, by Abcam (2 mentions) Ab11079, by Abcam (2 mentions) Ab8898, by Abcam (2 mentions) Ab8980, by Abcam (1 mentions) Anti h1.0 h5 clone 3h9, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Anti cleaved parp, by Abcam (1 mentions) Fibrillarin, by Abcam (1 mentions)

3 protocols using ab24175

Mapping Histone H1 Variants via ChIP-seq

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Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05–629-I), anti-H1.2 (Abcam, ab4086), anti-H1.4 (Invitrogen, 702876), anti-H1.5 (Invitrogen, 711912) and anti-H1X (Abcam, ab31972). ChIP-seq of H3K9me3 was performed using anti-H3K9me3 (Abcam, ab8898). Other antibodies used were: anti-H1.0 (Abcam, ab11079), anti-H1.3 (Abcam, ab24174), anti-H1.5 (Abcam, ab24175), anti-H3 (Abcam, ab1791) and anti-Lamin A (Abcam, ab8980).

Serna-Pujol N., Salinas-Pena M., Mugianesi F., Le Dily F., Marti-Renom M.A, & Jordan A. (2022). Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. Nucleic Acids Research, 50(7), 3892-3910.

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Immunostaining of Cell Markers

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Primary antibodies used were: NPM1 (mouse IgG1, clone FC82291, Sigma-Aldrich); β-actin (mouse IgG1, clone AC-15, Sigma-Aldrich); fibrillarin (rabbit, ab 5821 Abcam); cleaved caspase-3 at Asp175 (mouse, Cell Signaling); anti-cleaved PARP (rabbit MAb, clone E51, ab 32064, Abcam); H1.5 (rabbit, ab24175, Abcam); Glial fibrillary acidic protein (GFAP, Sigma-Aldrich); β-Tubulin III (TUJ1 clone, Covance); CNPase (monoclonal IgG1, clone 11-5B, Sigma-Aldrich). Secondary antibodies were: anti-rabbit and anti-mouse HRP conjugated antibodies (Amersham); anti-mouse and anti-rabbit conjugated with FITC or Texas Red (Vector).

Holmberg Olausson K., Elsir T., Moazemi Goudarzi K., Nistér M, & Lindström M.S. (2015). NPM1 histone chaperone is upregulated in glioblastoma to promote cell survival and maintain nucleolar shape. Scientific Reports, 5, 16495.

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Histone H1 Variant Identification

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Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam, ab4086), anti-H1.4 (Invitrogen, 702876), anti-H1.5 (Invitrogen, 711912) and anti-H1X (Abcam, ab31972). ChIP-seq of H3K9me3 was performed using anti-H3K9me3 (Abcam, ab8898). Other antibodies used were: anti-H1.0 (Abcam, ab11079), anti-H1.3 (Abcam, ab24174), anti-H1.5 (Abcam, ab24175) and anti-H3 (Abcam, ab1791).

Serna‐Pujol N., Salinas‐Pena M., Mugianesi F., Dily F.L., Martı́-Renom M.A., & Jordan A. (2021). Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion.

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