Lcms 8030 triple quadrupole mass spectrometer

Manufactured by Shimadzu

Sourced in Japan

The LCMS-8030 is a triple quadrupole mass spectrometer manufactured by Shimadzu. It is a high-sensitivity analytical instrument designed for the detection and quantification of a wide range of compounds in complex samples. The LCMS-8030 utilizes tandem mass spectrometry technology to provide reliable and accurate results.

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LCMS-8030 (68 citations) 8030 triple quadrupole mass spectrometer (13 citations) 8030 Mass Spectrometer (5 citations) LCMS-8030 mass spectrometer (4 citations) LCMS 8030 spectrometer (3 citations) LCMS-8030 quadrupole mass spectrometer (2 citations) Spectrometers (2 citations)

14 protocols using lcms 8030 triple quadrupole mass spectrometer

HPLC-MS/MS Analysis of Phenolics

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HPLC procedures chromatographic separation was performed on Aquasil C18 (Thermo Electron, Dreieich, Germany) column (150 mm × 3 mm, 3 μm particle size). The solvents used were (A) 0.1% formic acid in water and (B) 0.1% formic acid in methanol. The elution gradient established was 10-100% B, 0-45 min; 100% B, 45-55 min and reequilibration duration was 5 min between individual runs. The flow rate of the mobile phase was 0.4 mL/min, the injection volume was 5 μL, and the column temperature was maintained at 40°C. Phenolics present in the fractions were characterized according to their retention times, UV and mass spectra compared with commercial standards when available. The quantification of phenolics was determined based on DAD results, using 280 nm for the phenolic acids and 320 and 370 nm for flavonoids.
The LC-ESI-MS/MS analysis was carried out using a LCMS-8030 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan) equipped with an electrospray ionization (ESI). The mass spectrometer was operated in negative ion mode with a nebulizing gas flow of 1.5 L/min, a dry gas flow rate of 12 L/min, a block source temperature of 400°C, a DL (dissolving line) temperature of 250°C, the full scan spectra from 50 to 2000 Da, and the negative ionization mode source voltage-4500 V.

Ben Salah H., Smaoui S., Abdennabi R, & Allouche N. (2019). LC-ESI-MS/MS Phenolic Profile of Volutaria lippii (L.) Cass. Extracts and Evaluation of Their In Vitro Antioxidant, Antiacetylcholinesterase, Antidiabetic, and Antibacterial Activities. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 9814537.

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LC-MS/MS Analysis of Complex Matrices

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The extracts of complex matrices
were analyzed by means of an LCMS-8030 triple quadrupole mass spectrometer
(Shimadzu, Kyoto, Japan) using LabSolutions software (version 5.82).
The electrospray ionization (ESI) interface was operated with a syringe
pump (Legato Nano; KD Scientific, Holliston, MA) infusing the sample
at a flow rate of 30 μL min^–1. The nebulizing
gas (nitrogen) flow rate was 2 L min^–1, while the
drying gas (nitrogen) flow rate was 15 L min^–1.
The desolvation line temperature was 250 °C, while the heating
block temperature was 400 °C. The voltages of the ESI needle
were +4.5 and −3.5 kV in the positive- and negative-ion modes,
respectively. Depending on the experiment, the instrument was operated
in Q3 scan (m/z range, 20–2000),
product ion scan, or multiple reaction monitoring (MRM) mode. In the
case of product ion scan and MRM modes, different collision energies
were tested: −10, −20, and −30 V for positive
ions and 10, 20, and 30 V for negative ions. MRM transitions: 294.00
→ 250.00 for diclofenac, 152.00 → 110.00 for acetaminophen,
89.00 → 43.00 for lactic acid, 120.00 → 74.00 for threonine.
Instrumental blank signals were recorded for ∼2 min, followed
by recording sample signals for ∼2 min. The extracted ion currents
were obtained for 1 unit intervals in the m/z scale. The final values correspond to the average of extracted
ion currents from 1 min recording.

Liao P.H, & Urban P.L. (2019). Agarose-Based Gel-Phase Microextraction Technique for Quick Sampling of Polar Analytes Adsorbed on Surfaces. ACS Omega, 4(21), 19063-19070.

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LC-MS Analysis of Metabolites

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LC‐MS analysis was performed on a Shimadzu Nexera X2 system with an LC‐30AD binary pump, connected to a SIL‐30AC autosampler, CTO‐20AC column heater, SPD‐M30A diode array detector, and a Shimadzu LC‐MS 8030 triple quadrupole mass spectrometer. MS spectra were recorded in electron spray ionization (ESI) mode, scanned in a range of m/z = 100–1300 in the positive and negative ionization modes. Separation was accomplished by using the above‐mentioned HPLC column and 40 mm sodium formate buffer (pH 5, solvent A) and acetonitrile with the following gradient: 50% B for 15 min, to 69% B in 19 min, to 72% B in 12 min, to 82% in 7 min, and to 95% B in 10 min, which was held for another 10 min. The postrun time was set to 10 min and the column temperature to 25 °C. The flow rate was 0.6 mL·min⁻¹, and spectra were recorded from 10 to 73 min.

Kaltenegger E., Prakashrao A.S., Çiçek S.S, & Ober D. (2020). Development of an activity assay for characterizing deoxyhypusine synthase and its diverse reaction products. FEBS Open Bio, 11(1), 10-25.

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HPLC-MS Analysis of Metabolites

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HPLC–MS analysis was carried out with a Shimadzu LCMS-8030 triple quadrupole mass spectrometer equipped with an electrospray ionization interface and integrated into the UPLC system (Shimadzu, Kyoto, Japan). The optimization of the instrument settings was as follows: interface voltage, 4.5 kV; desolvation line temperature, 250 °C; heat block temperature, 400 °C; desolvation gas, nitrogen; desolvation gas flow rate, 3 L/min; drying gas, nitrogen; drying gas flow rate, 15 L/min; collision gas, argon; and collision gas pressure, 230 kPa. Multiple reaction monitoring (MRM) mode was used in the MS spectrometer operating parameters. The chromatographic separation was accomplished using a Purospher^® STAR RP-18 end-capped column (100 mm × 2.1 mm, two μm, Merck KGaA, Darmstadt, Germany). The column temperature was maintained at 40 °C. The following gradient program was used, along with a mobile phase consisting of 10 mM ammonium acetate in 0.1% formic acid (pH: 3.4) (solvent A) and methanol (solvent B): this initial term for 3 min in an isocratic elution composing with 60% solvent B; 3–8 min: 60–95% B; 8–13.5 min: 95–95% B; 13.5–15 min: 95–60% B; and 15–17 min: 60–60% B; v/v. The flow rate was 0.2 mL/min, and the injection volume was 10 μL.

Liu J.H., Cheng Y.Y., Hsieh C.H, & Tsai T.H. (2017). Identification of a Multicomponent Traditional Herbal Medicine by HPLC–MS and Electron and Light Microscopy. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(12), 2242.

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Carotenoid Identification via UFLC-LCMS

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The UFLC XR Prominence coupled with a LCMS-8030 triple quadrupole mass spectrometer (Shimadzu) was used for the identification of the carotenoids. The analysis was conducted through a Symmetry C₈ column (150 × 4.6 mm, 3.5 μm particle size, 100 Å pore size) (Waters, Milford, USA) with gradient elution using two solvents: solvent A (H₂O with 0.1% formic acid), and solvent B (methanol with 0.1% formic acid) at a flow rate of 1.5 mL/min. Each run of the LC-MS/MS was performed with isocratic elution with 90% solvent B for 2 min. The column oven temperature was 30 °C, the DL temperature was 250 °C, the nebulizing gas flow rate was 3 L/min, the heat block temperature was 400 °C, the drying gas flow rate was 15 L/min, the mass range was from 400 to 700 m/z, the cooler temperature was 5 °C, and the electrospray was in its ionization (ESI) mode. Optimization of the collision energy (CE) was carried out by multiple reaction monitoring (MRM) to find the optimal CE. The data were used for carotenoid species analysis. Identification of the pure carotenoids was based on the precursor ion, product ion, and single ion monitoring (SIM) data.

Setiyono E., H.e.r.i.y.a.n.t.o., Pringgenies D., Shioi Y., Kanesaki Y., Awai K, & Brotosudarmo T.H. (2019). Sulfur-Containing Carotenoids from A Marine Coral Symbiont Erythrobacter flavus Strain KJ5. Marine Drugs, 17(6), 349.

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LC-MS/MS Analysis of Estradiol

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The LC/ESI-MS/MS instrument was comprised of a Shimadzu LCMS-8030⁺ triple quadrupole mass spectrometer and a Shimadzu LC-30AD chromatograph (Kyoto, Japan). A YMC-UltraHT Pro C18 (2.0 µm, 100 × 2.0 mm i.d., Kyoto) was used at the flow rate of 0.3 mL/min and at the temperature of 40°C. The derivatized and intact E₂ were analyzed in the positive-ion and negative-ion modes, respectively. The MS/MS conditions common to all the compounds were as follows: interface voltage, 4.5 or −3.5 kV; detector voltage, 2.16 or −2.12 kV; nebulizer gas (N₂) flow rate, 3 L/min; drying gas (N₂) flow rate, 15 L/min; desolvation line temperature, 250°C; heat block temperature, 400°C; and collision gas (Ar), 230 kPa. The Q1 pre-rod bias voltage (Q1), Q3 pre-rod bias voltage (Q3), collision energy (CE), SRM transitions (precursor and product ions), and mobile phases (isocratic elution) for the respective compounds are described in Table 1. LabSolutions software (version 5.53 SP3, Shimadzu) was used for the system control and data processing.

Kaneko H., Matsuoka H., Ishige T., Kobayashi H, & Higashi T. (2023). Derivatization procedure of estradiol with a combination of MPDNP-F and 4-dimethylaminopyridine to generate product ion containing estradiol-skeleton for reliable determination of its serum/plasma concentrations by LC/ESI-MS/MS. Analytical and Bioanalytical Chemistry, 416(2), 597-608.

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Simultaneous UHPLC-DAD-MS Analysis of Compounds

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UHPLC-DAD-MS analyses were carried out on a Shimadzu Nexera 2 liquid chromatograph with a LC30AD binary pump, connected to a SIL-30AC autosampler, CTO-20AC column heater, SPD-M30A diode Array detector and a LC-MS 8030 triple quadrupole mass spectrometer using electrospray ionization (Shimadzu, Kyoto, Japan). The column used was a Phenomenex Luna Omega C18 (100 × 2.1 mm, 1.6 µm particle size) (Phenomenex, Aschaffenburg, Germany).
For analyses, 0.1% formic acid in LC-MS water (A) and acetonitrile (B) were used as solvents with the following gradient: 5% B to 95% B in 45 min held for 15 min. The post-run time was set to 10 min, the temperature to 30 °C. The flow rate was 0.2 mL/min. The injection volume was 2.00 μL. Base Peak Chromatograms (BPC) were recorded in the positive and negative ionization mode for a mass range of m/z 200–1500 and m/z 200–1000 respectively. PDA chromatograms were recorded at 360 and 254 nm.

Di Lecce R., Mérindol N., Pérez M.G., Karimzadegan V., Berthoux L., Boari A., Zidorn C., Vurro M., Surico G., Desgagné-Penix I, & Evidente A. (2022). Biochemical Analyses of Bioactive Extracts from Plants Native to Lampedusa, Sicily Minor Island. Plants, 11(24), 3447.

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Yin-Chen-Hao-Tang Bioactive Compound Analysis

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A Shimadzu UHPLC system consisting of a CBM-20A system controller, LC-20AD XR pumps, DGU-20A₃ degasser, SIL-20AC XR auto sampler, and CTO-20A column oven coupled with an electrospray ionization (ESI) interface equipped with an LCMS-8030 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan) were utilized for the separation and detection of bioactive compounds in Yin-Chen-Hao-Tang and carbamazepine (IS). The remote-controlled software for the Shimadzu UHPLC-MS/MS system (Kyoto, Japan) was LabSolutions v. 5.60 SP1. Statistical calculations were performed using Microsoft Excel.

Hsueh T.P., Lin W.L, & Tsai T.H. (2016). Using Light Microscopy and Liquid Chromatography Tandem Mass Spectrometry for Qualitative and Quantitative Control of a Combined Three-Herb Formulation in Different Preparations. Molecules, 21(12), 1673.

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LCMS-Based Identification of Phenolic Compounds

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An LCMS-8030 triple quadrupole mass spectrometer (Shimadzu Scientific Instruments, Columbia, SC, USA) with an electrospray ionization (ESI) source was used for mass spectrometric analysis. Samples were dissolved in MeOH and adjusted to a concentration of 10 µg mL⁻¹.
The following data refer to the settings for the identification of 2″-O-galloyl hyperoside. A mixture of water and MeOH containing 0.1% acetic acid (50:50) with a flow rate of 0.6 mL min⁻¹ was used as mobile phase. The following ESI interface conditions were set: nebulization gas flow at 3 L min⁻¹, desolvation line temperature at 250 °C, heating block temperature at 400 °C and drying gas flow at 15 L min⁻¹. Nitrogen served as nebulizer and argon as collision gas. The ESI ion source was used in negative and positive modes. A scan in a range m/z 100 up to 1000 was performed first, followed by MRM (multiple reaction monitoring) optimization, in which the five most intense MRM transitions were analyzed. For the identification of 7-methyl juglone, the ESI interface conditions were set as follows: nebulization gas flow at 3 L min⁻¹, desolvation line temperature at 300 °C, heating block temperature at 500 °C and drying gas flow at 10 L min⁻¹. The other conditions were the same as for 2″-O-galloyl hyperoside.

Gerschler S., Guenther S, & Schulze C. (2022). Antibiofilm Activity of Sundew Species against Multidrug-Resistant Escherichia coli Strains. International Journal of Molecular Sciences, 23(22), 13720.

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Quantification of PGD2 in Lung Tissue

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PGD₂ levels in lung tissues were measured as described previously [28 (link)]. Specifically, excised lung was quickly frozen in liquid nitrogen and homogenized using Cryo-Press CP-50W (MICROTEC, Chiba, Japan). Tissues were then mixed with ethanol, centrifuged (800 g, 10 min), and the supernatant was diluted with 15% ethanol (in deionized water) containing 0.15% HCl (5 mol/l). PGD₂-d4 was added as internal standard. The solutions were applied to Sep-Pak Vac 3 cc cartridges (Waters, Milford, MA). The cartridges were washed by n-hexane, and the samples were eluted with ethyl acetate and reconstituted in 10% acetonitrile (in deionized water). The samples were then applied to, and quantified by LCMS-8030 triple Quadrupole mass spectrometer (Shimadzu, Kyoto, Japan). Liquid chromatography was performed by using the Kinetex C18 column (Phenomenex, Torrance, CA).

Kida T., Ayabe S., Omori K., Nakamura T., Maehara T., Aritake K., Urade Y, & Murata T. (2016). Prostaglandin D2 Attenuates Bleomycin-Induced Lung Inflammation and Pulmonary Fibrosis. PLoS ONE, 11(12), e0167729.

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