Morphology was studied following standard histological procedures (10 (link), 11 (link)). Briefly, articular specimens were fixed (4% formaldehyde, 5 days) and immersed in Osteosoft decalcifier solution (Merck) for 5 to 8 weeks. Then, samples were embedded in paraffin, 5-μm-thick serial sections were obtained in the middle part of the scaffolds (~3 mm diameter), and stained with hematoxylin-eosin and Masson's trichrome. The presence of chondral glycosaminoglycan was monitored by Alcian blue staining (pH 2.5) and counterstained with Harris hematoxylin. Sections were analyzed under a Leica-DM4000B optical microscope, and pictures were taken using a camera (Leica-DFC420). Cell density (number of cells/mm2) was quantified inside the implanted scaffolds as well as the distance between articular surface and scaffold, using Image proPlus 7.0 (Media Cybernetics). Results are expressed as average values ± SD of 4 measurements. Results were compared using Mann-Whitney U-test, and were considered statistically significant when the p value was <0.05. The square of the Pearson correlation coefficient (R2) was used to determine the degree of linear regression between the parameters studied.
Osteosoft decalcifier solution
Manufactured by Merck Group
Sourced in United States
Osteosoft decalcifier solution is a laboratory product designed for the decalcification of bone and other calcified tissue samples. It is a chemical solution used to remove calcium salts from specimens, enabling easier sectioning and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Image pro plus 7, by Media Cybernetics (1 mentions)
Dfc420, by Leica (1 mentions)
Entellan, by Merck Group (1 mentions)
Safranin o solution, by Merck Group (1 mentions)
Fast green fcf, by Merck Group (1 mentions)
Eosin y solution, by Merck Group (1 mentions)
Weigert s iron hematoxylin, by Merck Group (1 mentions)
2 protocols using osteosoft decalcifier solution
1
Histological Evaluation of Scaffold Implants
2
Histological Evaluation of Rat Joints
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After micro-CT analysis, the hind paws and tails of the rats were decalcified with OsteoSoft decalcifier solution (no. 101728; Merck, Burlington, MA, USA) and embedded in paraffin. Five micrometer sections were stained for hematoxylin and eosin (H&E) or Safranin O Fast Green. For H&E staining, sections were incubated in Mayer’s hematoxylin (no. MHS16) and Eosin Y solution (no. HT110116) (both from Sigma, St. louis, MO, USA). For Safranin O Fast Green staining, sections were incubated in Weigert’s iron hematoxylin (no. HT1079), 0.1% fast green FCF (no. F7252), and 0.1% Safranin O solution (no. HT90432) (all from Sigma, St. louis, MO, USA). The sections were subsequently dehydrated and embedded in Entellan (no. 1.07961.0100; Merck, Burlington, MA, USA). Separate experimentally blinded observers scored the caudal spine, calcaneus, and tarsal regions semi-quantitatively (0–4) for inflammation, bone proliferation and pannus formation.
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