Stannous octoate

Diphenylmethane-4,4′-diisocyanate (MDI), stannous octoate, S-nitrosoglutathione (GSNO), and l-glutathione (GSH) are all obtained from Sigma-Aldrich. Polycarbonatediol (PCDL) is purchased from UBE Industries, Ltd. 2-Hydroxyethyl disulfide (BHS) is purchased from TCI. 1,4-Butylene glycol (BDO) and N, N-Dimethylacetamide (DMAc) are obtained from Kelong Chemical Reagent Co., Ltd (Chengdu, China) and distilled under vacuum before use.

PLCL was synthesized and subjected to a gel-pressing method to fabricate sheet-form scaffolds, as described elsewhere [25 (link)]. Briefly, PLCL (50:50) was synthesized at 150 °C for 24 h in the presence of Stannous Octoate (1 mmol, Sigma, St. Louis, MO, USA) as a catalyst. Dissolved PLCL (5% w/v, in Chloroform) was mixed homogeneously with approximately 300 μm sized salts to form the PLCL gels. The residual solvents were removed by placing the samples under vacuum for an additional 24 h. The salts were then leached out by immersing the samples in distilled, deionized water and subjecting them to constant shaking for 3 days. The resulting scaffolds (disk type, diameter = 9 mm and thickness = 3 mm) were freeze-dried for 24 h, and then sterilized with ethylene oxide gas. The porosity of the scaffolds was measured by a mercury intrusion porosimeter (Auropore IV; MicroMeritics, Norcross, GA, USA).

Lactic acid (LA), glycolic acid (GA), stannous octoate, hydroxyl-terminated PEG, combretastatin A4 disodium phosphate (CA4P) and epirubicin (EPI) were purchased from Sigma–Aldrich (USA). Primary antibodies, including anti-PCNA, anti-CD34, anti-CD31, anti-MTA1, anti-TGF-β, anti-CRT and anti-HMGB1, were obtained from Abcam (Cambridge, UK). Antibodies for flow cytometric analysis, including anti-CD45, anti-CD11c, anti-CD86, anti-CD11b, anti-Gr-1, anti-CD3, anti-CD4, anti-CD8 and anti-FOXP3 were purchased from Biolegend. The 4′,6-diamidino-2-phenylindole (DAPI) and TUNEL assay kit were purchased from Thermo Fisher Scientific, Waltham, MA, USA.

L-lactide (optical purity of 95%) was obtained from Daigang Biomaterial Co., Ltd. (Jinan, China). Polyethylene glycol (average Mn = 6000) was purchased from Aladdin Chemistry (Shanghai, China). Stannous octoate was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Perfluoropentane (PFP) was purchased from JenKem Technology Co., Ltd. (Beijing, China). Trichloromethane, diethyl ether, salicylic acid, absolute ethyl alcohol, Tween 80, Span 80, and lauryl sodium sulfate (SDS) were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China).

PLLA (average Mw: 230 kDa) was provided by Total Corbion (Amsterdam, Netherlands). L-Lactide was purchased from DURECT Co. (Birmingham, AL, USA). DL-lactide was obtained from Tokyo Chemical Ind. (Tokyo, Japan). Magnesium hydroxide (MH, 99.5%), ε-caprolactone, fibrinogen from human plasma, stannous octoate, sodium dodecyl sulfate (SDS), L-(+)-lactic acid (L-Lac), and 1-octanol were obtained from Sigma Aldrich (Burlington, MA, USA). Platelet concentrates (5 × 10⁴ platelets per μL) were purchased from the Korean National Red Cross (Seoul, Korea). IL-6 and IL-8 enzyme-linked immunosorbent assay (ELISA) kits were obtained from R&D Systems (Minneapolis, MN, USA).
HCAECs were acquired from Cambrex (Walkersville, MD, USA) and EGM-2 media with an MV bullet kit (Lonza, Basel, Switzerland). A cell-counting kit (CCK-8) was obtained from Dongin LS (Seoul, Korea). Phosphate-buffered saline (PBS) solution was supported from Hyclone (GE Healthcare Life Sciences, Boston, MA, USA). A Micro BCA^TM Protein assay kit was acquired from Thermo Scientific TM (Waltham, MA, USA). All chemicals were laboratory reagent grade and used without purification.