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Formalin

Manufactured by Xilong

Formalin is a chemical solution primarily composed of formaldehyde, water, and methanol. Its core function is to preserve biological specimens by fixing and hardening tissues, preventing decomposition and maintaining the structural integrity of the samples.

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2 protocols using formalin

1

Borneol and Menthol Analgesic Effects

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Medical Borneol was obtained from Shanghai Hongqiao Traditional Chinese Medicine Co., Ltd. (+)‐Borneol (simply called Borneol in this study), (±)‐menthol (simply called menthol in this study), complete Freund's adjuvant, and capsaicin were purchased from Sigma‐Aldrich. Formalin was purchased from Xilong Chemical Co. AMTB, naloxone, and LY341495 were obtained from TOCRIS. Bicuculline was obtained from Tokyo Chemical Industry. Muscimol was obtained from Enzo Life Sciences. ATP disodium salt was obtained from Sangon Biotech. Ionomycin was purchased from Cayman Chemical. Morphine hydrochloride was obtained from Northeast Pharm.
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2

Stabilizing Ear Vessel Tissue for Microscopy

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To keep the shape of the blood vessels before it was made into slices, the piece of ear was moved into the formalin (37.0–40.0%, Xilong Scientific) immediately to stabilize the ear vessel tissue after the flowing observation experiment. After the sequences of water scrubbing, dehydration, transparency, waxdip, embedding, slice up and slice fixation, the venule slice was stained. The stained slice was dehydrated, sealed and finally fabricated. The procedures were performed according to the standard method as described in previous studies34 (link).
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