Microbank cryovial

Manufactured by Pro-Lab Diagnostics

Sourced in United Kingdom, Canada

Microbank cryovials are specialized containers designed for the long-term storage and preservation of biological samples, such as microorganisms and cell cultures, at ultra-low temperatures. These vials are made of high-quality materials and feature a secure closure system to maintain sample integrity during freezing and thawing processes.

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Lab products found in correlation

Middlebrook 7h11 agar, by BD (1 mentions) Maldi biotyper system, by Bruker (1 mentions) Chromid vre agar, by bioMérieux (1 mentions) Maldi tof, by Bruker (1 mentions) Vitek 2, by bioMérieux (1 mentions) Tween 80, by Merck Group (1 mentions) Api 32 e system, by bioMérieux (1 mentions) Vitek gni card, by bioMérieux (1 mentions) Vitek 2 instrument, by bioMérieux (1 mentions) Maldi tof ms system, by Bruker (1 mentions) Mueller hinton agar medium, by Merck Group (1 mentions)

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Microbank (80 citations)

8 protocols using microbank cryovial

M. chimaera Identification and Characterization

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Mycobacteria from positive MGIT cultures were first identified as NTM by Genotype CM (Bruker, Germany) and then identified as M. chimaera by Genotype NTM-DR (Bruker, Germany). Positive MGIT were subcultured in Middlebrook 7H11 Agar (7H11 plate, Becton Dickinson, USA) for approximately 2 weeks, in order to obtain cultures of “pure” M. chimaera. All the isolated M. chimaera strains were stored at −20°C using Microbank cryovial (Pro-Lab Diagnostics, Canada), after further identification by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry using MALDI Biotyper system (Bruker, Germany).
As requested by the Italian Ministry of Health as part of the national surveillance program, unfrozen M. chimaera strains were subcultured in a new MGIT and sent to the National Institute from Infectious Diseases “L. Spallanzani” (INMI) to carry out molecular epidemiological investigation. Subsequent DNA extraction, WGS, and bioinformatic analysis were then performed at INMI.

Bisognin F., Messina F., Butera O., Nisii C., Mazzarelli A., Cristino S., Pascale M.R., Lombardi G., Cannas A, & Dal Monte P. (2022). Investigating the Origin of Mycobacterium chimaera Contamination in Heater-Cooler Units: Integrated Analysis with Fourier Transform Infrared Spectroscopy and Whole-Genome Sequencing. Microbiology Spectrum, 10(6), e02893-22.

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Detecting Vancomycin-Resistant Enterococci

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Rectal swabs were collected at admission and on all inpatients on the haematology unit developing febrile neutropenia (neutrophils <0.9 × 10⁹/L or <1.0 × 10⁹/L and falling after chemotherapy, plus body temperature ≥38°C). Swabs were plated to CHROMID^® VRE agar (bioMérieux, Marcy-l’Étoile, France), species identification and vancomycin resistance were confirmed with MALDI–TOF (Microflex instrument, Bruker, Billerica, USA) and VITEK-2 (bioMérieux) with EUCAST breakpoints. All purple colonies from VREfm positive plates were stored at −80°C in a Microbank cryovial (Pro-Lab Diagnostics, Birkenhead, UK). Any VREfm isolated from clinical samples within 60 days of a rectal positive were also stored. Patient metadata were retrieved from electronic records and movements visualized with HAIviz v.0.3 (https://haiviz.beatsonlab.com/). This work was approved by the NHS Scotland BioRepository Network (ref. TR000126) and the University of St Andrews Research Ethics Committee (ref. MD12651).

McHugh M.P., Pettigrew K.A., Taori S., Evans T.J., Leanord A., Gillespie S.H., Templeton K.E, & Holden M.T. (2024). Consideration of within-patient diversity highlights transmission pathways and antimicrobial resistance gene variability in vancomycin-resistant Enterococcus faecium. Journal of Antimicrobial Chemotherapy, 79(3), 656-668.

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Entomopathogenic Fungus Culture Preparation

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Two strains of B. bassiana (I-2960, I-2961) and one strain of B. hoplocheli (B507), were obtained from Arysta LifeScience. The strains were stored at -80°C using Microbank cryovials (Pro-Lab Diagnostics, Richmond Hill, Canada). Cultures were grown from the cryovial stored strains to prepare spore suspensions for the tests. All cultures were grown at 25°C on potato dextrose agar (PDA) medium until sporulation was observed (three to four weeks). Spore suspensions were prepared by scraping the surface of sporulated cultures and suspending conidia in a sterile solution of 0.05% TWEEN^® 80 (Sigma-Aldrich, St. Louis, MO, USA). Conidia suspensions were adjusted to 10⁶ or 10⁸ conidia mL^-1 using a Malassez hemocytometer. To determine conidia viability and the number of conidia per milliter, 100 μL of the conidia suspension was plated onto PDA, incubated at 25°C and the colony forming units were counted after five days.

Rohrlich C., Merle I., Mze Hassani I., Verger M., Zuin M., Besse S., Robène I., Nibouche S, & Costet L. (2018). Variation in physiological host range in three strains of two species of the entomopathogenic fungus Beauveria. PLoS ONE, 13(7), e0199199.

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Identification and Characterization of ESBL and QnrS Genes

Cited 1 time

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The identification of the recovered enteric bacterial isolates was performed through traditional bacteriological methods and biochemical tests as guided in the Clinical and Laboratory Standards Institute/NCCLS⁷ guidelines with an API 32 E system (bioMerieux SA, Marcy l’Etoile, France) according to Wei and Charles.⁸ The isolates were stored at −80°C in MicroBank cryovials containing 20% glycerol (Pro-Lab Diagnostics, Round Rock, TX, USA). Control strains used in this study included K. Pneumoniae ATCC 700603, and E. coli ATCC 25922. The carriage of ESBL and QnrS gene was screened on 78 ESBL-positive isolates which included 42 strains of E. coli, 7 strains of Klebsiella spp, 24 strains of Salmonella spp, and 5 strains of Shigella spp respectively. These bacteria genera were chosen on their phenotypic resistance profiles toward β-lactams and fluoroquinolone antimicrobial tested as described in previous related research according to Gundran et al.^{9 (link)}

Ndukui J.G., Gikunju J.K., Aboge G.O., Mwaniki J.K., Maina J.N, & Mbaria J.M. (2022). Molecular Characterization of ESBLs and QnrS Producers From Selected Enterobacteriaceae Strains Isolated From Commercial Poultry Production Systems in Kiambu County, Kenya. Microbiology Insights, 15, 11786361211063619.

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Carbapenem-Resistant Gram-Negative Bacteria Surveillance

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A total of 365 unique CRE clinical isolates were randomly selected for testing. This set comprised carbapenem-resistant (defined as nonsusceptibility [intermediate or resistant] to ≥1 carbapenem) isolates collected between 2007 and 2020 for an ongoing carbapenem-resistant Gram-negative pathogen surveillance study (originally obtained from Singapore General Hospital’s Diagnostic Bacteriology Laboratory) and those which were submitted to the Singapore General Hospital Pharmacy Research Laboratory for antibiotic combination testing. The study isolates were representative of the strains frequently encountered in our region, including bacterial isolates collected from nonresidents/foreign patients seeking treatment in Singapore (16 (link), 17 (link)).
The bacterial genus and species were identified and confirmed as per the institution’s microbiology laboratory routine procedures, i.e., using Vitek GNI+ cards with the Vitek 2 instrument (bioMérieux, Hazelwood, MO) and/or matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system (BrukerDaltonik GmbH, Germany). Isolates were preserved in Microbank cryovials (Pro-Lab Diagnostics, Richmond Hill, ON, Canada) at −80°C and subcultured twice on Trypticase soy agar + 5% sheep blood plates (BD, Sparks, MD) before experimental testing.

Teo J.Q., Chang H.Y., Tan S.H., Tang C.Y., Ong R.T., Ko K.K., Chung S.J., Tan T.T, & Kwa A.L. (2023). Comparative Activities of Novel Therapeutic Agents against Molecularly Characterized Clinical Carbapenem-Resistant Enterobacterales Isolates. Microbiology Spectrum, 11(3), e01002-23.

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Preparation of Yogurt Starter Cultures

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Lactobacillus delbrueckii subsp. bulgaricus LB 340 and Streptococcus thermophilus TA 040 yogurt starters were purchased from Danisco (France). Stock cultures were kept at −70 °C using Microbank cryovials (Pro-Lab Diagnostics, UK).Before using bacteria in experiments, they were serially propagated three times in the appropriate medium (MRS for LB340 and M17 for TA040). A 1% inoculum was used, and the incubation period was 24 h at 37 °C in anaerobic conditions. Unless otherwise stated, cultures of each strain were taken at the end of the exponential phase of growth at cell densities of 5 × 10⁶ CFU/mL.
Danisco (Algiers, Algeria) kindly provided the food additive emulsifier polysorbate 80, which was used in all experiments at a final concentration of 1 % (w/v).

Ziar H, & Riazi A. (2022). Polysorbate 80 improves the adhesion and survival of yogurt starters with cholesterol uptake abilities. Saudi Journal of Biological Sciences, 29(8), 103367.

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Bacterial Strain Identification Protocol

Cited 2 times

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Eight strains of bacteria, namely Morganella morganii (MM), Staphylococcus warneri (SW), Lactobacillus plantarum (LP), Enterococcus faecium (EF), Enterococcus durans (ED), Lactococcus garvieae (LG), Staphylococcus epidermidis (SE), and Escherichia coli (EC) were obtained from Microbank® cryovials (Pro-Lab Diagnostics, UK) deposited at −80 °C^{17 (link)} and grown using a modified method previously described by our research team^{18 (link)}. In order to confirm the identification of selected species of microorganisms, one bead was inoculated on Petri dishes (Alchem, Poland) with solid Mueller Hinton Agar medium (Sigma Aldrich, Germany). A microbial loop (1 μL) of bacterial biomass was applied directly to the plate, according to the procedure recommended by the manufacturer. A further procedure consisting of a bacterial protein extraction protocol using microorganism identification analysis using the MALDI-TOF–MS technique and the MALDI Biotyper 3.0 platform (Bruker Daltonics, Bremen, Germany) has been described in previous works of our team^{18 (link),19 (link)}.

Mametov R., Sagandykova G., Monedeiro F., Florkiewicz A., Piszczek P., Radtke A, & Pomastowski P. (2024). Metabolic profiling of bacteria with the application of polypyrrole-MOF SPME fibers and plasmonic nanostructured LDI-MS substrates. Scientific Reports, 14, 5562.

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Pseudomonas aeruginosa Isolation from U-Bends

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U-bends were flushed with water prior to sampling. P. aeruginosa was recovered by swab sampling U-bend interiors via sampling ports using sterile cotton swabs (Venturi Transystem, Copan, Italy) dipped in 0.5% (w/v) sodium thiosulphate solution [3, 13] . C2 U-bends were sampled once weekly for 52 weeks (N¼520), whereas A&E U-bends were sampled immediately after decontamination, and 24 h and 48 h after decontamination for 52 weeks (N¼1560). Average bacterial densities were calculated from these samples.
Swab tips were suspended in 1 mL of sterile phosphate buffered saline, vortexed and serially diluted, and 100-mL aliquots were spread in duplicate on Colombia blood agar (CBA), Reasoner's 2A (R2A) agar and PSCN as described elsewhere [3] . Presumptive P. aeruginosa isolates were recovered on PSCN, purified and identified using matrix-assisted laser desorption ionizationetime of flight mass spectrometry (MALDI-TOF-MS) [3] . Isolates were stored at -80 C in Microbank cryovials (Prolab Diagnostics, Neston, UK). Unless otherwise stated, a single isolate from each sample was stored (see below).

Moloney E.M., Deasy E.C., Swan J.S., Brennan G.I., O'Donnell M.J, & Coleman D.C. (2020). Whole-genome sequencing identifies highly related Pseudomonas aeruginosa strains in multiple washbasin U-bends at several locations in one hospital: evidence for trafficking of potential pathogens via wastewater pipes. The Journal of hospital infection, 104(4).

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