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Anti rabbit mouse horseradish peroxidase conjugated secondary antibody

Manufactured by Beyotime
Sourced in China

The Anti-rabbit/mouse horseradish peroxidase-conjugated secondary antibody is a laboratory reagent used for the detection and visualization of primary antibodies raised against rabbit or mouse antigens. This secondary antibody is conjugated with the enzyme horseradish peroxidase, which can catalyze a colorimetric or chemiluminescent reaction for signal amplification and detection.

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2 protocols using anti rabbit mouse horseradish peroxidase conjugated secondary antibody

1

Antibody Procurement and Utilization

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CHIR99021 and rabbit anti-β-catenin antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). BIO was purchased from Sigma-Aldrich. β-Actin mouse monoclonal antibody was purchased from Beijing TransGen Biotech Co., Ltd (Beijing, China). Drosha antibody was purchased from Cell signaling (Danvers, MA). Alexa Fluor 555-labeled goat anti-rabbit IgG and anti-rabbit/mouse horseradish peroxidase-conjugated secondary antibody were obtained from the Beyotime Institute of Biotechnology (Jiangsu, China).
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2

Pluripotency Marker Expression Analysis

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PD0325901, DMSO, and mouse anti-GAPDH were purchased from Sigma-Aldrich. The primary antibodies used were rabbit anti-Nanog (CST, Danvers, MA, USA), rabbit anti-Klf4 (Boster, Wuhan, China), mouse-anti-c-Myc (Santa Cruz, CA, USA), goat anti-Tet1 (Santa Cruz), rabbit anti-5hmC (Active Motif, Carlsbad, CA, USA), rabbit anti-Ezh2 (Abcam, Cambridge, UK), rabbit anti-H3K27me3 (Abcam), mouse anti-Oct3/4 (Santa Cruz), and mouse anti-Sox2 (Santa Cruz). Alexa Fluor 555-labeled goat anti-rabbit/mouse IgG and anti-rabbit/mouse horseradish peroxidase-conjugated secondary antibody were obtained from the Beyotime Institute of Biotechnology (Nantong, Jiangsu, China).
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