Hnrnpm

Manufactured by OriGene

HnRNPM is a protein that plays a role in RNA processing and transport. It is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family and is involved in the regulation of mRNA splicing and stability.

Automatically generated - may contain errors

Lab products found in correlation

β actin, by Merck Group (2 mentions) Occludin, by Santa Cruz Biotechnology (1 mentions) Hnrnp f, by Santa Cruz Biotechnology (1 mentions) γ catenin, by Cell Signaling Technology (1 mentions) Smad3, by Cell Signaling Technology (1 mentions) P smad3, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) N cadherin, by BD (1 mentions) Ha hrp, by Roche (1 mentions) Gapdh, by GE Healthcare (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions)

2 protocols using hnrnpm

Western Blot and Immunofluorescence Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for Western blotting and immunofluorescence assays were as follows: hnRNPM (OriGene), hnRNPF (Santa Cruz Biotechnology, Inc.), hnRNPH1/H2 (Santa Cruz Biotechnology, Inc.), p-Smad3 (Cell Signaling), Smad3 (Cell Signaling), CD44 (R&D Systems and Santa Cruz Biotechnology, Inc.), E-cadherin (Cell Signaling Technology), γ-catenin (Cell Signaling Technology), occludin (Santa Cruz Biotechnology, Inc.), N-cadherin (BD), vimentin (NEOMarkers), β-actin (Sigma-Aldrich), and GAPDH (Millipore). The ESRP1 antibody was a gift from Dr. R. Carstens (University of Pennsylvania).

Xu Y., Gao X.D., Lee J.H., Huang H., Tan H., Ahn J., Reinke L.M., Peter M.E., Feng Y., Gius D., Siziopikou K.P., Peng J., Xiao X, & Cheng C. (2014). Cell type-restricted activity of hnRNPM promotes breast cancer metastasis via regulating alternative splicing. Genes & Development, 28(11), 1191-1203.

+ Open protocol

+ Expand

SDS-PAGE Protein Detection via Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole cell lysates or RNA pull-down samples were separated by 10% SDS-PAGE, transferred to a PVDF membrane (BioRad), and probed with the appropriate antibody. Primary antibodies used in western blots included HA-HRP (Roche Applied Science), hnRNPM (OriGene). GAPDH (GE) and β-actin (Sigma-Aldrich) were used as loading controls. After incubation with HRP-tagged secondary antibodies, if appropriate, blots were visualized via chemiluminescence (Thermo Fisher).

Harvey S.E., Xu Y., Lin X., Gao X.D., Qiu Y., Ahn J., Xiao X, & Cheng C. (2018). Coregulation of alternative splicing by hnRNPM and ESRP1 during EMT. RNA, 24(10), 1326-1338.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!