Drug containing PPy films were grown potentiostatically onto the device electrodes using a Bio-Logic SP-150 potentiostat. The dexamethasone electrosynthesis solution consisted of 0.2 M Pyrrole (Alfa Aesar, Ward Hill, MA) and 0.02 M dexamethasone 21 phosphate disodium salt (Sigma-Aldrich, used as received) in Milli-Q water of 18 MΩ/cm resistivity. The Indomethacine electrosynthesis solution consisted of 0.2 M Pyrrole and 0.01 M Indomethacin (Sigma-Aldrich) in acetonitrile. The acetylsalicylic acid electrosynthesis solution consisted of 0.2 M Pyrrole (Alfa Aesar, Ward Hill, MA) and 0.02 M acetylsalicylic acid (Sigma-Aldrich,) in Milli-Q water. The films were synthesized by applying a constant current of 0.1 mA for Indomethacin and 1 mA for acetylsalicylic acid and dexamethasone each for 10 minutes between the drug release electrode and the counter electrode. For drug release experiments, the counter and working electrodes were reversed. The amount of released DEX, ASA and Indomethacin was quantified by absorbance at 242 nm, 298 nm and 318 nm, respectively using an infinite M200 Pro plate reader (TECAN).
Pyrrole
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States, Belgium
Pyrrole is a heterocyclic organic compound with the chemical formula C4H5N. It is a colorless liquid with a distinctive odor. Pyrrole is used as a building block in the synthesis of various pharmaceuticals and other chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Aniline, by Merck Group (3 mentions)
Sp 150 potentiostat, by Bio-Logic (2 mentions)
Infinite m200 pro plate reader, by Tecan (2 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions)
Sodium acetate trihydrate, by Reanal (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Dexamethasone (dex), by Thermo Fisher Scientific (1 mentions)
Indomethacine, by Merck Group (1 mentions)
Pyrrole, by Merck Group (1 mentions)
Acetylsalicylic acid, by Merck Group (1 mentions)
Dexamethasone 21 phosphate disodium salt, by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Dexamethasone (dex), by Tecan (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Sodium chloride (nacl), by Carl Roth (1 mentions)
Chloroplatinic acid, by Merck Group (1 mentions)
Potassium chloride (kcl), by Carl Roth (1 mentions)
Disodium hydrogen phosphate, by Carl Roth (1 mentions)
23 protocols using pyrrole
1
Electrochemical Synthesis of Drug-Loaded Polypyrrole Films
2
Electrochemical Synthesis and Drug Release of PPy/DEX Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PPy/DEX films were grown potentiostatically onto the device electrodes using a Bio-Logic SP-150 potentiostat. The electrosynthesis solution consisted of 0.2 M Pyrrole (Alfa Aesar, Ward Hill, MA) and 0.1 M DEX (Sigma-Aldrich, DEXamethasone 21 phosphate disodium, used as received) in Milli-Q water of 18 MΩ/cm resistivity. The film was synthesized by applying a constant potential of 1 V for 10 min between the device and a counter electrode. For drug release experiments, the counter and working electrodes were reversed. The amount of released DEX was quantified by absorbance at 242 nm (a characteristic band of DEX) using an infinite M200 Pro plate reader (TECAN).
3
Electrochemical Detection of SARS-CoV-2 Spike Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were used as received without further purification. The chemicals were purchased as follows: pyrrole 98% − from Alfa Aesar (Germany), sulfuric acid (H2SO4; 96%) from Lachner (Czech Republic), nitric acid (HNO3, 63%), sodium hydroxide (NaOH, 98%), and chloroplatinic acid (H2PtCl6, 40% Pt) from Merck (Germany), potassium phosphate (KH2PO4, 98%) from Honeywell Riedel-de Haen (Germany), sodium chloride (NaCl, 99,5%), potassium chloride (KCl, 99,5%), and disodium hydrogen phosphate (Na2HPO4, 99%) from Carl Roth (Germany). SARS-CoV-2 spike glycoproteins were purchased from UAB Baltymas (Lithuania).
The experiment was performed using potentiostat/galvanostat Metrohm-Autolab model μAutolabIII/FRA2 μ3AUT71079 controlled by NOVA 2.1.3 software (EcoChemie, The Netherlands). All measurements were done in a homemade cell. The total volume of the cell was 250 μL. Three-electrode system consisted of a working electrode (WE) – Pt disk with 1 mm diameter sealed in glass, reference electrode (RE) – Ag/AgCl, and counter electrode (CE) – Pt disk of 2 mm diameter.
The experiment was performed using potentiostat/galvanostat Metrohm-Autolab model μAutolabIII/FRA2 μ3AUT71079 controlled by NOVA 2.1.3 software (EcoChemie, The Netherlands). All measurements were done in a homemade cell. The total volume of the cell was 250 μL. Three-electrode system consisted of a working electrode (WE) – Pt disk with 1 mm diameter sealed in glass, reference electrode (RE) – Ag/AgCl, and counter electrode (CE) – Pt disk of 2 mm diameter.
4
Oxidative Pulp Treatment Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paper pulp used in this study was kindly supported by Chung-Hua Paper Inc. (Hualien, Taiwan). Hypochlorite (NaClO, 12%), sodium hydroxide (NaOH, 96%), sodium bromide (NaBr, >99%), sodium ammonium persulfate (APS, 98%), hydrochloric acid (HCl, 35%), and potassium bromide (KBr, 99%) were purchased from Showa Corporation (Saitama, Japan). Acrylic acid (AA, 98%) and iron (III) chloride hexahydrate (FeCl3·6H2O, 99%), were purchased from ACROS Organics (Geel, Belgium). 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO, 98%+), 2-acrylamido-2-methylpropane sulfonic acid (AMPS, 98%), N,N′-methylenebisacrylamide (MBAA, 97%), pyrrole (Py, 98%), N,N,N’,N’-tetramethylethylenediamine (TMEDA, 99%), and ethylenediaminetetraacetic acid (EDTA, 99%) were used as received from Alfa Aesar (MA, US) without further purification.
5
Synthesis of Formylbenzoate Dipyrromethane
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methyl 4-formylbenzoate and pyrrole were
purchased from Alfa Aesar and used as received. CH2Cl2 was dried and distilled over P2O5.
Iron perchlorate salts (M(ClO4)n·xH2O, M = FeII and FeIII) were used as received from Alfa Aesar. 5-Mesityldipyrromethane
was synthesized using the literature method.8a
purchased from Alfa Aesar and used as received. CH2Cl2 was dried and distilled over P2O5.
Iron perchlorate salts (M(ClO4)n·xH2O, M = FeII and FeIII) were used as received from Alfa Aesar. 5-Mesityldipyrromethane
was synthesized using the literature method.8a
6
Polymer Synthesis and Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pyrrole (98%, Alfa Aesar), ferric chloride hexahydrate (FeCl3·6H2O, 98%, Sigma-Aldrich), methyl orange (MO, ACS reagent, dye content 85%, Sigma-Aldrich), chloroform (CHCl3, HPLC grade, Fisher Scientific), PU (Tecoflex™ SG-80A, Thermedics), PLLA (intrinsic viscosity 1.3, Hycail). All chemicals except Pyrrole were used as received. Pyrrole was distilled and kept at 4 °C before use.
7
Multifunctional Bioactive Chitosan Hydrogel
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poly(ε-caprolactone) diol (PCL, Mn 2000 Da, Sigma-Aldrich, St. Louis, MO, USA), isophorone diisocyanate (IPDI, Acros, Geel, Belgium), Sn(Oct)2 (Alfa Aesar, Haverhill, MA, USA), 2,2-bis(hydroxymethyl) propionic acid (DMPA, Sigma-Aldrich, St. Louis, MO, USA), triethylamine (TEA, J.T. Baker, Phillipsburg, NJ, USA), ethylenediamine (EDA, Tedia, Fairfield, OH, USA), glyoxal (Alfa Aesar, Haverhill, MA, USA), chitosan (170 kDa, 97% deacetylation, Kiotec, Hsinchu, Taiwan), acrylic acid (Showa, Gyoda, Japan), NaOH (Sigma-Aldrich, St. Louis, MO, USA), methacrylic anhydride (MAA, Sigma-Aldrich, St. Louis, MO, USA), acetic acid (Fluka, Buchs, Switzerland), pyrrole (Alfa Aesar, Haverhill, MA, USA), oxidant ammonium persulfate (APS, Sigma-Aldrich, St. Louis, MO, USA), HCl (Showa, Gyoda, Japan), high glucose Dulbecco’s modified Eagle’s medium (HG-DMEM, Gibco, Waltham, MA, USA), fetal bovine serum (FBS, Gibco, Waltham, MA, USA), Ham’s F-12 (Gibco, Waltham, MA, USA), G418 (Invitrogen, Waltham, MA, USA), penicillin-streptomycin-amphotericin (PSA, Caisson Laboratories, Inc., Smithfield, UT, USA), cell counting Kit-8 (CCK-8, Sigma-Aldrich, St. Louis, MO, USA), and KAPA SYBR Green qPCR kit (Kapa Biosystems, Inc., Wilmington, MA, USA) were applied in commercial form.
8
Electrochemical Sensor Fabrication Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4-Nitrobenzaldehyde (99%), tin(ii ) chloride dihydride (SnCl2·2H2O, 98+%) were purchased from Acros Organics, terephthaloyl chloride (99%) and Nafion solution (5% w/w in H2O and 1-propanol) were obtained in Alfa Aesar, pyrrole (98%), pyridine (99%), sulfuric acid (H2SO4), potassium hydroxide (KOH) and 1.0 M PBS buffer (pH = 7) were purchased from Fisher Scientific and used without further purification. All the glassware and cells were decontaminated by soaking in aqua regia solution (conc. HCl/conc. nitric acid = 3 : 1). The glass apparatus was washed with deionized water and oven dried. Carbon paper substrate was purchased from Fuel Cell Store (AvCarb P75T −40 × 40 cm), which is carbon particle-filled, polyacrylonitrile (PAN) based carbon fiber paper with hydrophobic Teflon wet-proof (13% wt treatment) coating nominal thickness of 0.255 mm (@ 1 psi/0.7 N cm−2).
9
Curcumin-Loaded Carbon Nanotube Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CNTs were purchased
from C-nano Technology (China). Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione],
poly(vinyl alcohol) (PVA), sulfuric acid (H2SO4), ferric chloride hexahydrate (FeCl3· 6H2O), nitric acid (HNO3), and ethanol (C2H5OH) were procured from Showa Chemical Industry
(Japan). Pyrrole was procured from Alfa Aesar (UK), which was distilled
under reduced pressure prior to use.
from C-nano Technology (China). Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione],
poly(vinyl alcohol) (PVA), sulfuric acid (H2SO4), ferric chloride hexahydrate (FeCl3
(Japan). Pyrrole was procured from Alfa Aesar (UK), which was distilled
under reduced pressure prior to use.
10
Synthesis and Evaluation of Novel Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1,4-Butanediamine, di-tert-butyl dicarbonate ((BOC)2O), pyrrole, 2-pyrrolecarbaldehyde,
boron fluoride etherate (BF3·O(Et)2), sodium
carbonate (Na2CO3), sodium sulfate (Na2SO4), sodium hydroxide (NaOH), and triethylamine (Et3N) were purchased from Alfa Aesar company.l -Tryptophan
(l -Trp), l -cysteine (l -Cys), l -lysine (l -Lys), l -glutamic (l -Glu) acid,
sodium borohydride (NaBH4), MPA, trisodium citrate dihydrate,
tetrahydrofuran (THF), phosphorus oxychloride (POCl3),
cyanuric chloride (TCT), dimethyl sulfoxide (DMSO), N,N′-dicyclohexylcarbodiimide (DCC), carbodiimide
(EDC), N-hydroxy succinimide (NHS), 2-(N-morpholino) ethanesulfonic acid (MES), and fluorescein isothiocyanate
(FITC) were purchased from Shanghai Reagent Company. Fetal bovine
serum (FBS) and RPMI-1640 media were from Invitrogen Corporation.
All organic solvents were purchased from EM Sciences. MCF-7 (human
breast cancer cells), HepG2 (human hepatocellular liver carcinoma
cells), and QSG-7701 (human normal hepatocyte) cells were purchased
from the Shanghai Institute for Biological Sciences, Chinese Academy
of Sciences (Shanghai, China).
boron fluoride etherate (BF3·O(Et)2), sodium
carbonate (Na2CO3), sodium sulfate (Na2SO4), sodium hydroxide (NaOH), and triethylamine (Et3N) were purchased from Alfa Aesar company.
(
sodium borohydride (NaBH4), MPA, trisodium citrate dihydrate,
tetrahydrofuran (THF), phosphorus oxychloride (POCl3),
cyanuric chloride (TCT), dimethyl sulfoxide (DMSO), N,N′-dicyclohexylcarbodiimide (DCC), carbodiimide
(EDC), N-hydroxy succinimide (NHS), 2-(N-morpholino) ethanesulfonic acid (MES), and fluorescein isothiocyanate
(FITC) were purchased from Shanghai Reagent Company. Fetal bovine
serum (FBS) and RPMI-1640 media were from Invitrogen Corporation.
All organic solvents were purchased from EM Sciences. MCF-7 (human
breast cancer cells), HepG2 (human hepatocellular liver carcinoma
cells), and QSG-7701 (human normal hepatocyte) cells were purchased
from the Shanghai Institute for Biological Sciences, Chinese Academy
of Sciences (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!