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Deoxynivalenol

Manufactured by Pribolab
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Sourced in China

Deoxynivalenol is an analytical standard used for the detection and quantification of the mycotoxin deoxynivalenol in various matrices. It is a crystalline solid substance derived from Fusarium fungi.

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Lab products found in correlation

Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Nickel nitrilotriacetic acid ni nta sepharose, by Solarbio (1 mentions) Colormixed protein marker, by Solarbio (1 mentions) 96 well microplates, by Sangon (1 mentions) Deoxynivalenol, by Merck Group (1 mentions) Anti zo 1, by Abcam (1 mentions) Anti β actin antibody, by Cell Signaling Technology (1 mentions) Rabbit anti claudin 1, by Abcam (1 mentions) Rabbit anti sqstm1, by Cell Signaling Technology (1 mentions) Rabbit anti lc3b antibody, by Merck Group (1 mentions) Anti jak1, by Cell Signaling Technology (1 mentions) Anti occludin, by Abcam (1 mentions) Anti mapks, by Cell Signaling Technology (1 mentions) Anti pi3k, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp conjugated goat anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions) Sb203580, by MedChemExpress (1 mentions) Zearalenone, by Pribolab (1 mentions) Infinite m200 pro, by Tecan (1 mentions)

3 protocols using deoxynivalenol

1

Nanobody-Based Ochratoxin A Detection

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AAP was obtained from Psaitong (Beijing, China). AA was obtained from Sinopharm (Beijing, China). Colormixed protein marker and nickel-nitrilotriacetic acid (Ni-NTA) Sepharose were obtained from Solarbio (Beijing, China). OTA, ochratoxin B (OTB), ochratoxin C (OTC), zearalenone (ZEN), aflatoxin B1 (AFB1), fumonisin B1 (FB1), and deoxynivalenol (DON) standards were procured from Pribolab (Qingdao, China). 3,3′,5,5′-Tetramethyl benzidine (TMB), p-nitrophenylphosphate (pNPP), ovalbumin (OVA), bovine serum albumin (BSA), and 96-well microplates were obtained from Sangon Biotech (Shanghai, China). Tetramethylammonium hydroxide (TMA·OH) and manganese chloride tetrahydrate (MnCl2·4H2O) were purchased from Aladdin (Shanghai, China). Polyvinylidene fluoride membrane was obtained from Millipore (Bearrica, MA). OTA–BSA conjugates were prepared previously in the laboratory. The recombinant pET25b-Nb-G53Q&S102D vector containing the mutated Nb gene for OTA and the recombinant pecan45-Nb28-AP vector were prepared in previous works.24,33 (link)
Zhang Z., Su B., Xu H., He Z., Zhou Y., Chen Q., Sun Z., Cao H, & Liu X. (2021). Enzyme cascade-amplified immunoassay based on the nanobody–alkaline phosphatase fusion and MnO2 nanosheets for the detection of ochratoxin A in coffee. RSC Advances, 11(35), 21760-21766.
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2

Investigating Molecular Mechanisms of PEDV

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Deoxynivalenol (DON, purity ≥ 98%, for experiments in vitro), chloroquine (CQ), rapamycin (Rapa) and rabbit anti-LC3B antibody were purchased from Sigma-Aldrich (St. Louis, USA). Deoxynivalenol (DON, purity ≥ 98%, for experiments in vivo) was purchased from Pribolab (Immunos, Singapore). SB203580 and AUD-S100 were purchased from MedChemExpress (New Jersey, USA). Rabbit anti-SQSTM1, anti-MAPKs, anti-JAK1, anti-pSTING/STING, anti-PI3K, anti-β-actin antibodies and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody were purchased from Cell Signaling Technology (Boston, USA). Rabbit anti-claudin1, anti-occludin, anti-ZO-1 and anti-p-MTORC1/MTORC1 antibodies were purchased from Abcam (Cambridge, UK). Porcine epidemic diarrhea virus (PEDV) strain CV777 was obtained from Jiangsu Academy of Agricultural Sciences (Nanjing, China). Rabbit anti-PEDV-N antibody was prepared by our lab. Poly (I:C) (LMW) / LyoVecTM was purchased from InvivoGen (San Diego, USA).
Liu D., Ge L., Wang Q., Su J., Chen X., Wang C, & Huang K. (2020). Low-level contamination of deoxynivalenol: A threat from environmental toxins to porcine epidemic diarrhea virus infection. Environment International, 143, 105949.
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3

Mycotoxin Detection Using QD-Nanobody

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Restriction enzymes and PrimeSTAR® HS DNA polymerase were supplied by Takara Biomedical Tech. Co. Ltd. (Beijing, China). The primers listed in Table S1 were synthesized by Sangon Biotech (Shanghai, China). Gluconic acid, N, N-dicyclohexyl carbodiimide (DCC), N, N-dimethyl formamide (DMF), and N-hydroxysuccinimide (NHS) were obtained from Aladdin (Shanghai, China). Standards of mycotoxins, including aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FB1), OTA, ochratoxin B (OTB), ochratoxin C (OTC), and zearalenone (ZEN) were purchased from Pribolab (Qingdao, China). The ZnCdSe/ZnS QDs modified with an amino group (RQD-NH2, λem=614 nm) were supplied by Jiayuan Tech. (Wuhan, China). The recombinant vectors, pET25b-Nb28 encoding the Nb gene against OTA and pGEM-T-SGFP encoding the SGFP gene, were previously prepared in our laboratory. The remaining inorganic chemicals and organic solvents were of analytical grade or purity.
A spectral scanning multimode reader was used to scan the fluorescence spectra and UV–vis spectra (Infinite M200 Pro, Tecan, Switzerland). A PerkinElmer Frontier infrared spectrometer was used to analyze the Fourier transform infrared spectroscopy (FTIR) spectra (PerkinElmer, Waltham, MA, USA).
Su B., Zhang Z., Sun Z., Tang Z., Xie X., Chen Q., Cao H., Yu X., Xu Y., Liu X, & Hammock B.D. (2021). Fluonanobody-Based Nanosensor Via Fluorescence Resonance Energy Transfer for Ultrasensitive Detection of Ochratoxin A. Journal of hazardous materials, 422, 126838.
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