Gfp mouse antibody

Manufactured by Roche

The GFP mouse antibody is a laboratory reagent used to detect the presence of green fluorescent protein (GFP) in genetically modified cells or organisms. It is a highly specific and sensitive tool for tracking and visualizing GFP-expressing cells or proteins in various experimental settings.

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Lab products found in correlation

4 aminopyridine, by Bio-Techne (1 mentions) Brain derived neurotrophic factor (bdnf), by R&D Systems (1 mentions) Mouse flag m2 antibody, by Merck Group (1 mentions) Sar405, by Merck Group (1 mentions) Bicuculline, by Merck Group (1 mentions)

2 protocols using gfp mouse antibody

Western Blot Analysis of CREST Protein

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CREST was detected in pre-cleared lysates separated by SDS-PAGE and blotted as described previously [96 (link)]. For analysis of proteins in total lysate vs. supernatant and pellet, the pellet from 90 μg of total lysate centrifuged at 50K rpm for 6 min was resuspended in water to the initial volume of total lysate centrifuged. The same sample volumes of total lysate, supernatant and pellet were then separated by SDS-PAGE. Blots were developed with GFP mouse antibody from Roche Applied Science (Indianapolis, IN) and PGK antibody from life technologies (Frederick, MD).

Park S., Park S.K., Watanabe N., Hashimoto T., Iwatsubo T., Shelkovnikova T.A, & Liebman S.W. (2019). Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies. PLoS Genetics, 15(8), e1008308.

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Antibodies and Pharmacological Agents for Neuronal Imaging

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The following antibodies were used: VAMP2 rabbit antibody (#104202; Synaptic System), SV2 mouse antibody (Developmental Studies Hybridoma Bank), Flag M2 mouse antibody (#1804; Sigma-Aldrich), tubulin mouse antibody (#t9026; Sigma-Aldrich), mCherry rabbit antibody (#5993; BioVision), Myc mouse antibody (9E10; Santa Cruz), Hrs rabbit antibody (D7T5N; Cell Signaling), Hrs mouse antibody (ab56468; Abcam), GFP mouse antibody (#11814460001; Roche), KIF13A rabbit antibody (PA5-30874; Thermo Fisher Scientific), KIF13B mouse antibody (6E11; Santa Cruz), neurofilament and β-actin mouse antibodies (2H3 and 8-7A5; Developmental Studies Hybridoma Bank). Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 μg/μl, 24 h), bicuculline (Sigma-Aldrich, 40 μM, 5 min-2h or 20 h), 4-aminopyridine (Tocris Bioscience, 50 μM, 5 min-2h or 20 h), BDNF (R&D Systems, 50 ng/ml, 1 h), TTX (Tocris Bioscience, 0.5 μM, 2 h), Rapamycin analogue linker drug (AP21967; Clontech, 100 nM, 3 h), SAR405 (Millipore Sigma-Aldrich, 1 μM, 24 h), and Janelia Fluor 549 SNAPtag ligand (Janelia Research Campus, 200 nM, 30 min). Unless otherwise indicated, all other chemicals were purchased from Sigma-Aldrich.

Birdsall V., Kirwan K., Zhu M., Imoto Y., Wilson S.M., Watanabe S, & Waites C.L. (2022). Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation. Life Science Alliance, 5(10), e202000745.

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