Bifidobacterium breve

Manufactured by American Type Culture Collection

Sourced in United States

Bifidobacterium breve is a type of bacteria that can be used in laboratory settings. It is a Gram-positive, anaerobic, and non-spore-forming bacterium belonging to the genus Bifidobacterium.

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4 protocols using bifidobacterium breve

Probiotic and Pathogenic Strain Interactions

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The clinical strains used belonged to Candida albicans (strain SC5314), C. tropicalis and E. coli (both isolated from the fecal sample of a CD patient), Serratia marcescens, Trichosporon inkin, and Saccharomyces fibuligera.
The following probiotic strains were purchased from the American Type Culture Collection: Saccharomyces boulardii ATCC MYA-796, Lactobacillus acidophilus ATCC 43121, Bifidobacterium breve ATCC 15701, and L. rhamnosus ATCC 39595. Yeast nitrogen base (YNB) and brain heart infusion (BHI) media were purchased from Becton, Dickinson and Company (Sparks, MD). Hanks’ buffered saline solution (HBSS), phosphate-buffered saline (PBS), and fetal bovine serum (FBS) were purchased from Mediatech, Inc. (Manassas, VA).

Hager C.L., Isham N., Schrom K.P., Chandra J., McCormick T., Miyagi M, & Ghannoum M.A. (2019). Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms. mBio, 10(2), e00338-19.

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Activation and Cultivation of Bifidobacterium Species

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Bifidobacterium longum (ATCC BAA-999), and Bifidobacterium breve (ATCC 15700) were purchased directly from American Type Culture Collection (ATCC) Manassas VA, U.S.A. B. longum and B. breve was activated from a glycerol stock. Bifidobacterium agar media was purchased from HiMedia, India. B. breve and B. longum were maintained in standard Bifidobacterium agar medium at pH 5.5 ± 0.2.During inoculum preparation, B. breve and B. longum were grown in Bifidobacterium broth for 18 h at 37 °C, 120 rpm incubator shaker under anaerobic condition followed by centrifugation at 10,000 × g for 10 min at 4˚C.

Parhi P., Song K.P, & Choo W.S. (2022). Growth and survival of Bifidobacterium breve and Bifidobacterium longum in various sugar systems with fructooligosaccharide supplementation. Journal of Food Science and Technology, 59(10), 3775-3786.

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Culturing Diverse Anaerobic Bacteria

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Bacteria strains used are either purchased from ATCC (Lactobacillus acidophilus ATCC^® 4356^™, Lactobacillus paracasei ATCC^® BAA-52^™, Lac-tobacillus reuteri ATCC^® 23272^™, Lactococcus lactis ATCC^® 19435^™, Bifidobacterium breve ATCC^® 15700^™, Bifidobacterium longum subsp. longum ATCC^® 15707^™, Clostridium perfringens ATCC^® 13124^™, Ruminococcus gnavus 35913^™) or obtained from the Sonnenburg lab (Bacteroides fragilis NCTC 9343, Bacteroides finegoldii DSM 17565, Bacteroides dorei DSM17855, Bacteroides ovatus ATCC 8483, Lactobacillus plantarum ATCC BAA-793, Lactobacillus rhamnosus ATCC 53103).
Lactobacilli MRS Agar plate (Hardy Diagnostic, USA) was used to culture L. acidophilus, L. paracasei, L. reuteri, L. plantarum, L. rhamnosus, L. lactis. Blood Agar plate (Hardy Diagnostic, USA) was used to culture B. breve, B. longum, C. perfringens, R. gnavus. BHI agar plate (Hardy Diagnostic, USA) was used to culture B. finegoldii, B. dorei, B. ovatus, B. fragilis.
Each bacterial strain was inoculated onto the respective culture plates under sterile conditions. The plates were then placed in an air-tight gas pouch containing one pack of BD Difco^™ GasPak^™ EZ Gas Generating System (ThermoFisher B260683, USA) to create an anaerobic environment. The pouches were incubated at 37 °C for 48 hrs until harvest, allowing the bacteria to grow and develop.

Zhu B., Bai Y., Yeo Y.Y., Lu X., Rovira-Clavé X., Chen H., Yeung J., Gerber G.K., Angelo M., Shalek A.K., Nolan G.P, & Jiang S. (2024). A Spatial Multi-Modal Dissection of Host-Microbiome Interactions within the Colitis Tissue Microenvironment. bioRxiv.

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Tartary Buckwheat Probiotic Evaluation

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Tartary buckwheat (Chuanqiao No. 1) was provided by the Key Laboratory of Miscellaneous Grains Processing, Ministry of Agriculture and Rural Affairs, Chengdu University. The lyophilized powder of bacterial strains, Bifidobacterium longum (ATCC 15707), Bifidobacterium breve (ATCC 15700), Bifidobacterium adolescentis (ATCC 15703), and Lactobacillus rhamnosus (ATCC 53103) was procured from Mingzhou Biotechnology Co., Ltd.

Xiang Y., Cao Y., Yang S., Ren Y., Zhao G., Li Q., Li H, & Peng L. (2022). Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota. Food Science & Nutrition, 11(1), 408-417.

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