Dsq 2 gc

To understand which phytochemicals are present in the methanolic extract (MEC) and n-butanol fraction of Chrozophora tinctoria (NBFC), both samples were subjected to gas chromatography–mass spectrometry [33 (link)]. The plant samples were checked using a Thermo Scientific (DSQ-II) GC, furnished with a 30 m long TR-5MS capillary column and a 0.25 µm thick film with 0.25 mm of internal diameter. Helium was used as a carrier gas with a flow rate of 1 mL/min. The injection device was run in a split mode at 250 °C. The sample was injected, 1 µL at a time, with an initial oven temperature of 50 °C that was maintained for 2 min followed by gradually elevating the temperature to 150 °C at a rate of 8 °C/min. Ultimately, the temperature was increased to 300 °C at a speed of 15 °C/min and sustained for 5 min [34 (link),35 ]. The mass spectrometry was performed in full scan mode to obtain information about mass fragments and mass/charge (m/z) ratio in the range of 50–600.

Plant extracts exhibiting promising antibacterial activity were selected for GC-MS profiling. Plant samples with good MIC value were further analyzed for quantitative phytochemical analysis using “Thermo Scientific (DSQII) GC”. The GC was equipped with a TR-5MS capillary column of length 30 M, Fill Thickness 0.25 μm and Internal Diameter of 0.25 mm. The carrier gas Helium (He) was used with flow rate of 1 ml/min. The injector was operated in split mode with temperature of 250 °C.
The sample volume 1 μl was injected with initial Oven temperature of 50 °C and held for 2 min, then increased to 150 °C with the temp rate of 8 °C/min and further increased to 300 °C with temperature rate of 15 °C/min and hold for 5 min.