Omnitrace ultra water

Manufactured by Merck Group

Sourced in United States

The OmniTrace Ultra Water is a laboratory water purification system designed to produce high-purity water for a variety of analytical applications. It utilizes a multi-stage filtration process to remove impurities and provide consistent, reliable water quality.

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Lab products found in correlation

Apo transferrin, by Merck Group (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Ciprofloxacin, by Merck Group (1 mentions) Sodium l ascorbate, by Merck Group (1 mentions) Imipenem, by Merck Group (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions)

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OmniTrace Ultra (4 citations)

3 protocols using omnitrace ultra water

Preparation of Antimicrobial and Iron Chelating Agents

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Imipenem was bought from Sigma-Aldrich (USA) and was freshly prepared before each experiment. A stock solution of 5 mg/mL was prepared in RPMI 1640 medium (Lonza, USA), divided into aliquots, and stored at –20°C.
Sodium l-ascorbate was purchased from Sigma-Aldrich (USA) and was also prepared as a fresh stock solution of 5 mg/mL of OmniTrace Ultra Water (EMD Millipore Corporation).
Apo-transferrin (iron depleted) was purchased from Sigma-Aldrich (USA). It was also prepared fresh before each experiment in the form of stock solution (5 mg/mL) prepared in OmniTrace Ultra Water (EMD Millipore Corporation).

, & Al-Madboly L.A. (2022). A Novel Triple Combination To Combat Serious Infections with Carbapenem-Resistant Acinetobacter baumannii in a Mouse Pneumonia Model. Microbiology Spectrum, 10(5), e02710-21.

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Preparation of Apo-transferrin, Ciprofloxacin, and Meropenem

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Apo-transferrin (iron-depleted, Sigma–Aldrich, catalogue number T1147) was made fresh for each experiment. Stock solutions of Apo-transferrin were made in OmniTrace Ultra Water (EMD Millipore Corporation, catalogue number WX0003-6).
Ciprofloxacin (Sigma–Aldrich, catalogue number 17850) was freshly prepared for each experiment. Stock solutions of Ciprofloxacin (5 mg/mL) were made in 0.05 M HCl prepared with OmniTrace Ultra Water.
Meropenem (APP Pharmaceuticals, catalogue number 350720) was freshly prepared for each experiment. Stock solutions (50 mg/mL) were made in RPMI 1640 medium (Thermo Fisher Scientific, catalogue number 11875085).

Luna B.M., Ershova K., Yan J., Ulhaq A., Nielsen T.B., Hsieh S., Pantapalangkoor P., Vanscoy B., Ambrose P., Rudin S., Hujer K., Bonomo R.A., Actis L., Skaar E.P, & Spellberg B. (2019). Adjunctive transferrin to reduce the emergence of antibiotic resistance in Gram-negative bacteria. Journal of Antimicrobial Chemotherapy, 74(9), 2631-2639.

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Preparation of Trace Metal Solutions

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Solutions were prepared from TraceSELECT hydrochloric acid (Fluka), 99.999% pure aluminum chloride hydrate (Sigma-Aldrich), and OmniTrace Ultra water (EMD Millipore), as described in (15 (link)).

Legg B.A., Voïtchovsky K, & De Yoreo J.J. (2022). Hydroxide films on mica form charge-stabilized microphases that circumvent nucleation barriers. Science Advances, 8(35), eabn7087.

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