Tov 21g

The human ovarian cancer cell line, TOV-21G (p53-WT), was purchased from American Type Culture Collection (ATCC). The human ovarian cancer cell lines A2780 (p53-WT), COV318 (p53-I195F), and COV362 (p53-Y220C) were purchased from the European Collection of Authenticated Cell Cultures (ECACC). TOV-21G and A2780 were cultured in Roswell Park Memorial Institute medium (RPMI) (Welgene, Daegu, Korea). COV318 and COV361 were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich; St. Louis, MO, USA). Both media contained 10% fetal bovine serum (HyClone; GE Healthcare), 100 U/mL penicillin, and 100 µg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Cell lines were maintained in 5% CO₂ and 37 °C humidified incubators. Cells were subcultured every 3–4 days.

Human ovarian cancer cell lines (SK-OV-3, CA-OV-3, OVCAR3, and TOV-21G) were purchased from the American Type Culture Collection (Manassas, VA, USA). SK-OV-3, CA-OV-3, OVCAR3, and TOV-21G cells were grown in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin (WelGENE, Daegu, South Korea) in a humidified atmosphere of 5% CO₂ at 37 °C.