OSCC cell lines (HSC2, HSC3, HSC4, SAS and Ca9-22) and HaCaT were obtained from the Cell Bank (RIKEN BioResource Center, Tsukuba, Japan). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 µg/ml streptomycin and 100 U/ml penicillin (Thermo Fisher scientific) at 37°C in a humidified atmosphere containing 5% CO2.
Hsc 4
Manufactured by RIKEN BioResource Center
Sourced in Japan
The HSC-4 is a laboratory equipment designed for the cultivation and storage of hematopoietic stem cells (HSCs). It provides a controlled environment for the propagation and preservation of these specialized cells. The core function of the HSC-4 is to enable the maintenance and expansion of HSCs in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Hsc 3, by RIKEN BioResource Center (14 mentions)
Penicillin, by Thermo Fisher Scientific (7 mentions)
Streptomycin, by Thermo Fisher Scientific (7 mentions)
Ca9 22, by RIKEN BioResource Center (5 mentions)
Hsc 2, by RIKEN BioResource Center (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Ho 1 u 1, by RIKEN BioResource Center (3 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Rpmi 1640, by Nacalai Tesque (2 mentions)
Calu 1, by American Type Culture Collection (2 mentions)
Jurkat, by American Type Culture Collection (2 mentions)
Ca9 22, by Nacalai Tesque (2 mentions)
Hsc 4, by Nacalai Tesque (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Hacat, by RIKEN BioResource Center (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
C57bl 6 mice, by Kerafast (1 mentions)
Minimal essential medium, by Nacalai Tesque (1 mentions)
19 protocols using hsc 4
1
Culturing OSCC Cell Lines
2
Oral Squamous Cell Carcinoma Cell Lines
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Human cell lines derived from OSCC HSC-3 and HSC-4 cells were obtained from Riken BRC Cell Bank (Tsukuba, Japan). To analyze the association between SOX9 expression and metastasis, two cell lines, namely HSC-3, a metastatic cell line that was established from the metastatic lymph node of a 63-year-old man with poorly differentiated SCC and HSC-4, a non-metastatic cell line that was established from a metastatic lymph node in a 63-year-old man with well-differentiated SCC, were selected (21 (link)). The cells were maintained in Minimum Essential Medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal calf serum (FCS) under a humidified atmosphere with 5% CO2 and 95% air at 37°C.
3
Establishment and Characterization of HNSCC Cell Lines
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HNSCC cell lines HSC-3, HSC-4 (tongue SCC, DR1/4) and Sa-3 (gingival SCC, DR9/10) were provided by the RIKEN Bio-Resource Center (Tsukuba, Japan). CA9-22 (gingival SCC) and HPC-92Y (hypopharyngeal SCC) were kindly provided by Dr. Yasuharu Nishimura (Dep. of Immunogenetics, Kumamoto University, Kumamoto, Japan) and Dr. Syunsuke Yanoma (Yokohama Tsurugamine Hospital, Yokohama, Japan), respectively. SAS (tongue SCC), Calu-1 (non-small cell lung carcinoma) and 5637 (bladder cancer) were purchased from American Type Culture Collection (Manassas, VA). All cell lines were maintained in RPMI 1640 (nacalai tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum.
4
Oral Squamous Cell Carcinoma Cell Lines
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HSC4 (tongue SCC) was supplied by the RIKEN BioResource Center (Tsukuba, Ibaraki, Japan). HPC-92Y (hypopharyngeal SCC) was kindly provided by Dr. Syunsuke Yanoma (Yokohama Tsurugamine Hospital, Yokohama, Japan). MOC1 (tongue SCC derived from C57BL/6 mice) was supplied by Kerafast Inc. (Boston, MA, USA). All cell lines were maintained by tissue culture with RPMI1640 (Nacalai tesque, Japan), 10% FBS (Sigma-Aldrich, Burlington, MA, USA), and Penicillin-Streptomycin (Gibco, Waltham, MA, USA). All cell lines were used within 10 passage after obtaining from the distributors. C57BL/6 mice (female, 8 to 10 weeks old) were purchased from Charles River Laboratories Japan, Inc. (Yokohama, Japan). All the mice were maintained in a specific pathogen-free facility at the Asahikawa Medical University. The experimental protocol was approved by the Institutional Animal Care and Use Committee of Asahikawa Medical University (#20001).
5
Establishing HLA-DR Expressing Cell Lines
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Mouse fibroblast cell lines that were transfected with plasmids expressing individual human HLA-DR molecules (L-DR4, −9 or −53) were kindly provided by Dr. Robert W Karr (Karr Pharma, Saint Louis, MO) and by Dr. Takehiko Sasazuki (Kyushu University, Fukuoka, Japan). The cell lines HSC3 (tongue squamous cell carcinoma SCC, DR15/15), HSC4 (tongue SCC, DR1/4, −53), Sa-3 (gingival SCC, DR9/10, −53) and Lu65 (lung large cell carcinoma, DR4/15, −53) were supplied by the RIKEN Bio-Resource Center (Tsukuba, Japan). The HNSCC cell line HPC92Y (hypopharyngeal SCC, DR4/9, −53) was kindly provided by Dr. Syunsuke Yanoma (Yokohama Tsurugamine Hospital, Yokohama, Japan). Tumor cell lines SAS (tongue SCC, DR9/15, −53), Calu-1 (Lung SCC, DR7/14, −53), WiDr (colon adenocarcinoma, DR4/7, −53) and Jurkat (T cell lymphoma, a cell line not expressing HLA-DR) were purchased from the American Type Culture Collection (Manassas, VA, USA). All cell lines were maintained in a tissue culture medium as recommended by the supplier.
6
Cultivation of Human Oral Cancer Cell Lines
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The human oral squamous cell carcinoma (OSCC) cell lines SAS (#RCB1974), HSC-3 (#RCB1975), HSC-4 (#RCB1902), and Ca9-22 (#RCB1976) were purchased from RIKEN BRC Cell Bank (Tsukuba, Japan; 2013), where the cell lines were authenticated by STR profiling before distribution. Cells were cultured in Minimum Essential Medium (MEM, Invitrogen/GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (Nichirei Bio., Tokyo, Japan) in a humidified atmosphere containing 5% CO2 at 37°C according to supplier’s instructions. Cells were used within 6 months of resuscitation.
7
Authentication of Gingival and Tongue Cancer Cell Lines
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The human gingival cancer cell line Ca9-22 was obtained from the Japanese Collection of Research Bioresources Cell Bank and the human tongue cancer-derived cell line HSC-4 was obtained from the RIKEN BioResource Center. As it has been reported that some stocks of Ca9-22 are contaminated with MSK-922 (19 (link)), short tandem repeat analysis was performed for the Ca9-22 cell line by BEX Co., Ltd., and it was confirmed to be authentic. Ca9-22 cells were cultured in minimal essential medium (Nacalai Tesque, Inc.) supplemented with 600 mg/l glutamine and 10% heat-inactivated fetal bovine serum (FBS; Nichirei Biosciences, Inc.). HSC-4 cells were maintained in RPMI-1640 medium (Nacalai Tesque, Inc.) supplemented with 10% heat-inactivated FBS. Both media contained 100 IU/ml penicillin (Thermo Fisher Scientific, Inc.) and 100 mg/ml streptomycin (Thermo Fisher Scientific, Inc.). Cells were maintained at 37°C in an atmosphere containing 95% air and 5% CO2.
8
Gingival Epithelial Cell Culture
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Reagents. OCT (maxacalcitol) and 1α,25-dihydroxy vitamin D 3 (calcitriol) were provided by Chugai Pharmaceutical Corporation (Tokyo, Japan). 1α,25dihydroxy vitamin D 2 (ercalcitriol), an active metabolite of vitamin D 2 , was obtained from R&D Systems (Minneapolis, MN, USA). Other reagents were obtained from Sigma-Aldrich unless otherwise indicated.
Human gingival/oral epithelial cell lines. The human gingival epithelial cell line Ca9-22 established from squamous cell carcinoma was obtained from the Japanese Collection of Research Biosources Cell Bank (Hokkaido, Japan). The human oral epithelial cell lines HSC-2, HSC-3, and HSC-4 established from squamous cell carcinoma were obtained from RIKEN BioResource Center (Ibaraki, Japan). Ca9-22, HSC-2, HSC-3, and HSC-4 cells were grown in E-MEM (Nacalai Tesque, Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and 1% antibiotic-antimycotic mixture. Cells were incubated in medium containing 5% FBS unless otherwise indicated.
Human gingival/oral epithelial cell lines. The human gingival epithelial cell line Ca9-22 established from squamous cell carcinoma was obtained from the Japanese Collection of Research Biosources Cell Bank (Hokkaido, Japan). The human oral epithelial cell lines HSC-2, HSC-3, and HSC-4 established from squamous cell carcinoma were obtained from RIKEN BioResource Center (Ibaraki, Japan). Ca9-22, HSC-2, HSC-3, and HSC-4 cells were grown in E-MEM (Nacalai Tesque, Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and 1% antibiotic-antimycotic mixture. Cells were incubated in medium containing 5% FBS unless otherwise indicated.
9
HNSCC Cell Lines for Immunotherapy
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We used HNSCC cell lines HSC3 (tongue SCC, HLA‐DR 15), HSC4 (tongue SCC, HLA‐DR 9/10, 53), HPC92Y (hypopharyngeal SCC, HLA‐DR4/9, 53), and SAS (tongue SCC, HLA‐DR 9/15. 53). HSC3 and HSC4 were supplied by RIKEN BioResource Center. HPC‐92Y and L cells (mouse fibroblast cell lines) expressing individual human HLA‐DR molecules (HLA‐DR4, 9, 15, and 53) were provided by Dr. S. Yanoma (Yokohama Tsurugamine Hospital), Dr. R. Karr (Karr Pharma), and Dr. T. Sasazuki (Kyushu University). SAS cells were purchased from ATCC. All cell lines were maintained in tissue culture, as recommended by the supplier.
10
Establishment of OSCC Cell Lines
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Eight human OSCC cell lines (Ca922, HO-1-u-1, HSC2, HSC3, HSC4, SAS, HSQ89, and Sa-3) were purchased from the RIKEN BioResource Center (Ibaraki, Japan). The human keratinocyte HaCaT cell line was obtained from the Cell Line Service (Eppelheim, Germany) as a control. All cell lines were maintained in appropriate media (RPMI-1640 or Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.
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