Zsm710

Cells were fixed in 4% paraformaldehyde and washed with PBS. Cell specimens were stained with rhodamine phalloidin (Cytoskelton, Inc.) and mounted with DAPI (Vectashield; Vector Laboratories, Burlingame, CA, USA). Mounted cell specimens were analyzed with a confocal microscope (ZSM710; Carl Zeiss).

Cells were fixed in 4% paraformaldehyde and blocked in 5% skim milk/phosphate buffer saline with 0.1% Tween-20 (PBST). After washing in PBST, cell samples were incubated with rabbit anti-FAK(p397) antibody (Abcam, Cambridge, UK) or mouse anti-N-cadherin antibody (BD Biosciences, San Jose, CA, USA) in 5% skim milk/PBST, washed with PBST twice, and incubated with Akexa488-conjugated anti-rabbit IgG antibody or Alexa546-conjugated anti-mouse IgG antibody (Thermo Fisher Scientific, Waltham, MA, USA). Cell specimens were also stained with rhodamine phalloidin (Cytoskelton, Inc., Denver, CO, USA). Mounted cell specimens were analyzed with a confocal microscope (ZSM710; Carl Zeiss, Jena, Germany).