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Sas v 9.1

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SAS (version 9.1) is a software product developed by SAS Institute. It is a comprehensive analytical platform designed for data management, analysis, and reporting. The core function of SAS is to provide a integrated environment for statistical analysis, data visualization, and business intelligence.

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SAS 9.4 (20063 citations) SAS (v. 9.3.1) (4 citations) SAS V.9.1 for Windows (3 citations) SAS STAT v 9.1 for Windows (2 citations) SAS software for Windows (v.9.1; (2 citations) SAS v.9.1 statistical package (2 citations)

3 protocols using sas v 9.1

1

Boar Semen Replication Analysis

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Replicates were performed independently using semen pooled from different boars. At least 3 replicates were performed for each condition. Differences among means were determined using a one-way analysis of variance in SAS (v. 9.1 SAS Institute, Inc, Cary, NC). The results are shown as means ± SEM and the means were considered to belong to distinct populations if P < 0.05 using Tukey’s test for multiple comparisons.
Machado S.A., Sharif M., Kadirvel G., Bovin N, & Miller D.J. (2020). Adhesion to oviduct glycans regulates porcine sperm Ca2+ influx and viability. PLoS ONE, 15(8), e0237666.
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2

Sperm Binding to Oviduct Cell Aggregates

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Each sperm binding experiment was performed at least 3 times using triplicate droplets. Sperm bound to either >10 cell aggregates or 25–30 derivatized beads to assess sperm binding. The oviduct cell aggregates were obtained from 15–20 oviducts. Oviduct cells were screened for their ability to form aggregates and for ciliary beating. All experiments used semen pooled from 3–5 boars. Differences among means were determined using one-way analysis of variance in SAS (v. 9.1 SAS Institute, Inc, Cary, NC). A droplet containing sperm bound to either oviduct cell aggregates or beads was considered an experimental unit. The results are shown as means ± SEM and the means were considered to belong to distinct populations if P < 0.05 using Tukey’s test for multiple comparisons.
Sharif M., Kerns K., Sutovsky P., Bovin N, & Miller D.J. (2021). Progesterone Induces Porcine Sperm Release from Oviduct Glycans in a Proteasome-Dependent Manner. Reproduction (Cambridge, England), 161(4), 449-457.
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3

Sperm Binding Inhibition Assay

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Each sperm binding experiment was performed at least 3 times using triplicate droplets. Differences among means were determined using a one-way analysis of variance in SAS (v. 9.1 SAS Institute, Inc, Cary, NC). A droplet containing sperm bound to beads was considered an experimental unit. Percent inhibition of sperm release was calculated by dividing the difference between “before treatment” and “after pharmacological inhibitor is added” by the control treatment without any inhibitor. The results are shown as means ± SEM and the means were considered to belong to distinct populations if P < 0.05 using Tukey’s test for multiple comparisons.
Sharif M., Hickl V., Juarez G., Di X., Kerns K., Sutovsky P., Bovin N, & Miller D.J. (2022). Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans. Scientific Reports, 12, 6446.
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