Gold 3 chloride hydrate haucl4 3h2o

Titanium foil with a chemical purity of 99.7%, ammonium fluoride with chemical purity ≥98%, phosphate buffered saline (0.01 M PBS, pH 7.4), ethylene glycol (assay 99.8%), gold (III) chloride hydrate HAuCl₄∙3H₂O (assay 99.995%), gold nanoparticles (10 nm stabilized suspension in 0.1 mM PBS), bovine serum albumin (BSA, purity ≥ 98%), were purchased from Sigma-Aldrich (St. Louis, MO, USA). All of the solutions were prepared from Milli-Q water.

The following chemicals were obtained from Sigma Aldrich (USA): D-(+)-glucose (C₆H₁₂O₆, 99.5%), sulfuric acid (H₂SO₄, 98%), and gold (III) chloride hydrate (HAuCl₄·3H₂O, 99.9%). The Nafion™ perfluorinated resin solution was also purchased from Sigma Aldrich (USA). For the electrochemical oxidation of glucose, a neutral pH solution of 0.1 M phosphate-buffered saline (PBS) with a pH of 7.4 was prepared. The PBS solution was made by dissolving disodium hydrogen phosphate (Na₂HPO₄) and monopotassium phosphate (KH₂PO₄) in distilled water, which was obtained from SolvAchim (Casablanca, Morocco). A stock solution of 100 mM glucose was prepared in the 0.1 M phosphate buffer solution (PBS) and stored at 4 °C.