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Hesperidin c28h34o15

Manufactured by Merck Group
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Hesperidin (C28H34O15) is a flavonoid compound found in citrus fruits. It is a natural compound that can be extracted and used as a laboratory reagent. Hesperidin has a molecular formula of C28H34O15 and a molar mass of 610.57 g/mol. Its core function is as a chemical standard and reference material for analytical and research purposes.

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Lab products found in correlation

Hesperidin, by Merck Group (1 mentions) Primary antibody against actin, by Merck Group (1 mentions) 5 5 6 6 tetrachloro 1 1 3 3 tetraethylbenzimidazolylcarbocyanine iodide jc 1, by Thermo Fisher Scientific (1 mentions) Primary antibodies against caspase 3 and caspase 9, by Cell Signaling Technology (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Hoechst 33342 dye, by Merck Group (1 mentions)

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Hesperidin

4 protocols using hesperidin c28h34o15

1

Diesel Particulate Matter Toxicity Inhibition

Cited 2 times
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Hesperidin (C28H34O15) was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO). Standard diesel PM2.5 (SRM 1650b) issued by the National Institute of Standards and Technology (NIST, Gaithersburg, MD, USA) was purchased from Sigma-Aldrich Inc. and dissolved in DMSO. LY294002 (a phosphoinositide 3-kinase (PI3K) inhibitor), U0126 (a mitogen-activated protein kinase (MEK) inhibitor), SP600125 (a c-Jun N-terminal kinase (JNK) inhibitor), and SB203580 (a p38 inhibitor) were purchased from Calbiochem (San Diego, CA, USA) [17 (link),18 (link)].
Fernando P.D., Piao M.J., Kang K.A., Zhen A.X., Herath H.M., Kang H.K., Choi Y.H, & Hyun J.W. (2022). Hesperidin Protects Human HaCaT Keratinocytes from Particulate Matter 2.5-Induced Apoptosis via the Inhibition of Oxidative Stress and Autophagy. Antioxidants, 11(7), 1363.
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2

Hesperidin Modulates Diesel PM2.5 Effects

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Hesperidin (C28H34O15) and standard diesel PM2.5 (SRM 1650b) were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO). PM2.5 was issued by the National Institute of Standards and Technology (NIST, Gaithersburg, MD, USA), and the stock solution (25 mg/mL) was sonicated for 30 min to avoid agglomeration.
Herath H.M., Piao M.J., Kang K.A., Zhen A.X., Fernando P.D., Kang H.K., Yi J.M, & Hyun J.W. (2022). Hesperidin Exhibits Protective Effects against PM2.5-Mediated Mitochondrial Damage, Cell Cycle Arrest, and Cellular Senescence in Human HaCaT Keratinocytes. Molecules, 27(15), 4800.
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3

Hesperidin Preparation and Application

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The flavonoid hesperidin (C28H34O15, CAS number 520-26-3) was purchased from Sigma-Aldrich (St. Louis, MO, USA). For cell culture assays, hesperidin was diluted in dimethyl sulfoxide (DMSO; Sigma Chemical Co., St. Louis, MO, USA) and used at a final concentration of 5 μM or 10 μM, as specified bellow. For in vivo experiments, hesperidin was prepared as previously described (Donato et al., 2014 (link)). hesperidin was diluted in DMSO at a final concentration of 5%, a solution of 0, 25% polysorbate 80 at a final concentration of 20% and in saline solution to complete the total volume.
Matias I., Diniz L.P., Buosi A., Neves G., Stipursky J, & Gomes F.C. (2017). Flavonoid Hesperidin Induces Synapse Formation and Improves Memory Performance through the Astrocytic TGF-β1. Frontiers in Aging Neuroscience, 9, 184.
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4

Hesperidin Bioactivity Evaluation

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Hesperidin (C28H34O15), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, N-acetyl cysteine (NAC), 5,5-dimethyl-1-pyrroline-N oxide (DMPO), 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Hoechst 33342 dye and primary antibody against actin were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolylcarbocyanine iodide (JC-1) was purchased from Invitrogen (Carlsbad, CA, USA). Primary antibodies against Bcl-2-associated X protein (BAX) and B-cell lymphoma 2 (Bcl-2) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Primary antibodies against caspase-3 and caspase-9 were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals and reagents were of analytical grade.
Hewage S.R., Piao M.J., Kang K.A., Ryu Y.S., Han X., Oh M.C., Jung U., Kim I.G, & Hyun J.W. (2016). Hesperidin Attenuates Ultraviolet B-Induced Apoptosis by Mitigating Oxidative Stress in Human Keratinocytes. Biomolecules & Therapeutics, 24(3), 312-319.
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