Uvi link cl 508l

Before UVA irradiation, we pretreated all cells with different concentrations of EGT (0.125-0.5 μM) or vehicle (0.1% DMSO) for 24 h. Prior to incubation, PBS-washed cells were then resuspended in new phenol red-free DMEM containing 10% FBS. We further irradiated these cells with 3 J/cm² (for 27 min) of UVA (λ_max, 365 nm; no detectable emission below 320 nm) using the UVILink CL-508L (UVItec, Cambridge, UK). After this incubation period, cells were fixed and or harvested to perform subsequent experiments in this study. We used 50 μM of EGT solution prepared in PBS as our stock solution. It was also stored at -20°C.

HSF cells were treated with various concentrations of ZER (2, 4, or 8 μM) or vehicle (0.1% DMSO) for 24 h or 2 h. After treatments, cells were washed with phosphate-buffered saline (PBS) and resuspended in fresh phenol red-free DMEM containing 10% FBS. In our recent study, 3 J/cm² UVA dosage indicated less cytotoxicity (~90% cell viability) [41 (link)]. As such, we applied the same dosage throughout this study. Using an UVI link CL-508L (UVItec, Cambridge, UK), HSF cells were irradiated with 3 J/cm² UVA for about 27 min (λ_max 365 nm; no detectable emission below 320 nm). After irradiation, cells were allowed to be incubated for 2 h (for the expression of proteins) before proceeding to other experiments. A stock concentration of 50 mM ZER was prepared with DMSO and stored at -20°C for further analysis.