Dako automated immunostainer

Manufactured by Agilent Technologies

Sourced in Denmark

The Dako automated immunostainer is a laboratory instrument designed for the automated staining of tissue samples for immunohistochemical analysis. The core function of this equipment is to perform immunostaining procedures, which involve the detection and visualization of specific target proteins or antigens within tissue sections.

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Lab products found in correlation

Benchmark ultra automated immunostainer, by Roche (1 mentions) Nod scid gamma mice, by Jackson ImmunoResearch (1 mentions) Envision hrp kit, by Agilent Technologies (1 mentions) Mib 1, by Agilent Technologies (1 mentions)

Close spelling variants of this product

DAKO immunostainer Automated immunostainer

3 protocols using dako automated immunostainer

DLBCL Tumor Classification and Subtyping

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Classification and subtyping of all tumors followed the definitions of the 2008 WHO classification of DLBCL [19] . Immunostainings for CD3, CD5, CD20,CD79a, CD10, MUM1, BCL2, BCL6, kappa and lambda light chains and heavy chain IgM (all purchased by DAKO, Denmark) were performed using Dako automated immunostainer (DAKO, Denmark). Immunohistochemistry with anti-MYC (clone Y69, Ventana-Roche, Italy) was conducted using BenchMark Ultra automated immunostainer (Ventana Medical Systems, Tucson, AZ, USA). The Hans algorithm [22] (link) was used in order to classify cases as GCB-type or non GCB-type. Immunostaining results for BCL2 and MYC were recorded as the percentage of positive cells in increments of 10% regardless of the intensity of the staining. Cases were considered as negative if <5% of tumor cells were positive. Immunohistochemical and morphological analyses were independently evaluated by two experienced hematopathologists (LR, ADN). Disagreements were resolved by joint review on a multi-head microscope.

Cox M.C., Di Napoli A., Scarpino S., Salerno G., Tatarelli C., Talerico C., Lombardi M., Monarca B., Amadori S, & Ruco L. (2014). Clinicopathologic Characterization of Diffuse-Large-B-Cell Lymphoma with an Associated Serum Monoclonal IgM Component. PLoS ONE, 9(4), e93903.

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Breast Cancer PDX Model Development

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The following research protocol was approved by Northwestern University institutional review board, and all patients provided appropriate informed consent. Metastatic pleural effusion samples were obtained from breast cancer patients and de-identified. Metastatic pleural effusion cells were engrafted into the mammary fat pad of non-obese diabetic/severe combined immunodeficient (NOD/SCID) gamma (NSG) mice (Jackson Laboratory). When a PDX tumor reached 1 cm in its largest dimension, the mouse was euthanized and freshly resected 2 mm × 2 mm tumor pieces were re-transplanted to NSG mice for in vivo studies. A piece of PDX tumor was fixed in 10% formalin and processed to paraffin-embedding. Paraffin sections (5 μm) were stained with hematoxylin and eosin (H&E) for histopathological evaluation. Expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) in human primary and PDX breast tumors was detected by immunohistochemical staining in the Pathology Core Facility (PCF), Robert H. Lurie Comprehensive Cancer Center using a DAKO automated immunostainer and standard En-Vision-HRP kit (DAKO, Carpinteria, CA).

Ugolkov A., Gaisina I., Zhang J.S., Billadeau D.D., White K., Kozikowski A., Jain S., Cristofanilli M., Giles F., O’Halloran T., Cryns V.L, & Mazar A.P. (2016). GSK-3 inhibition overcomes chemoresistance in human breast cancer. Cancer letters, 380(2), 384-392.

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Quantifying Tumor Necrosis and Proliferation

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Tumor samples from mice were frozen and then formalin fixed and paraffin embedded (FFPE). Haematoxylin and eosin slides were used to evaluate the percentage of tumor necrosis. Immunohistochemistry for Ki-67 (clone MIB-1, Dako, Denmark) was carried out using the Dako automated immunostainer (DAKO, Denmark). Tumor proliferation index was assessed as the percentage of Ki-67-positive tumor nuclei.

Fattore L., Malpicci D., Marra E., Belleudi F., Noto A., De Vitis C., Pisanu M.E., Coluccia P., Camerlingo R., Roscilli G., Ribas A., Di Napoli A., Torrisi M.R., Aurisicchio L., Ascierto P.A., Mancini R, & Ciliberto G. (2015). Combination of antibodies directed against different ErbB3 surface epitopes prevents the establishment of resistance to BRAF/MEK inhibitors in melanoma. Oncotarget, 6(28), 24823-24841.

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