Cary 6000 spectrometer

Manufactured by Agilent Technologies

The Cary 6000 spectrometer is a high-performance analytical instrument designed for ultraviolet-visible-near infrared (UV-Vis-NIR) spectroscopy. It is capable of measuring absorption, transmission, and reflectance spectra within the wavelength range of 175 to 3,300 nanometers.

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Lab products found in correlation

D3024, by Scilogex (1 mentions) Arx400 spectrometer, by Bruker (1 mentions) Ltq xl lc ms, by Thermo Fisher Scientific (1 mentions) Ultima 4, by Rigaku (1 mentions) Quartz cuvette, by Thorlabs (1 mentions)

3 protocols using cary 6000 spectrometer

Isotope-Labeled Retinal Substitution in GtACR1

Cited 2 times

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The substitution of the native all-trans-retinal chromophore of GtACR1 with all-trans-[15-¹³C,15-²H]retinal and all-trans-[14,15-²H₂]retinal followed similar procedures described previously for CaChR1.^{11 (link)} Reconstituted GtACR1 membranes were suspended in 100 mM hydroxylamine buffer with 300 mM NaCl and 20 mM HEPES (pH 7) and exposed to 530 nm light-emitting diode (LED) illumination (5 mW/cm²) for 40 min. Bleaching was monitored by UV–visible spectroscopy using a Cary 6000 spectrometer (Agilent Technologies, Inc., Santa Clara, CA). After >95% conversion of the retinal chromophore to retinal oxime, the sample was pelleted using a SCILOGEX D3024 centrifuge spun at 21000g for 3 min and resuspended in wash buffer [300 mM NaCl and 20 mM HEPES (pH 7)]. This was repeated at least three times to remove unreacted/excess hydroxylamine and free retinal oxime. A 2-fold stoichiometric excess of the isotope-labeled retinal was then added as a 2 mM EtOH solution. The incorporation of the isotope-labeled retinal was verified by resonance Raman spectroscopy (RRS) using a Renishaw inVia confocal Raman microscope with 785-nm laser excitation similar to measurements previously reported.^{10 (link)}

Yi A., Li H., Mamaeva N., Fernandez De Cordoba R.E., Lugtenburg J., DeGrip W.J., Spudich J.L, & Rothschild K.J. (2017). Structural Changes in an Anion Channelrhodopsin: Formation of the K and L Intermediates at 80 K. Biochemistry, 56(16), 2197-2208.

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Detailed Characterization of Ligands 1 and 2

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The ¹H and ¹³C NMR spectra of the 1 and 2 ligands were
obtained in CDCl₃ solution at 25 °C on a Bruker ARX-400
spectrometer. Chemical shifts are reported relative to the residual
solvent signals. FTIR spectra were measured in the 4000–400
cm^–1 range on a PerkinElmer spectrum I spectrometer
with samples prepared as KBr pellets. The UV–vis spectra were
recorded using 1 mM solution on an Agilent Cary 6000 spectrometer.
Electrospray ionization (ESI) mass spectrometry was performed using
a Thermo Scientific LTQ XL LC–MS instrument for the 50–2000
amu range. PXRD data were recorded on a Rigaku Ultima IV diffractometer
equipped with 3 kW sealed-tube Cu Kα X-ray radiation (generator
power settings: 40 kV and 40 mA) and a D/tex Ultra detector using
the BB geometry over the angle range 5–50° with a scanning
speed of 2°/min with 0.02° step. (2.5° primary and
secondary solar slits and 0.5° divergence slit with 10 mm height
limit slit). The surface morphology of the as-prepared samples was
examined using FESEM (JEOL, 8 or 15 kV). Fluorescence spectra were
recorded using a HORIBA Jobin-Yvon fluorescence spectrophotometer
with stirring attachment. Lifetime measurements of 1 and 2 were carried out using the time-resolved HORIBA scientific
single photon counting controller.

Chakraborty G, & Mandal S.K. (2018). Design and Development of Fluorescent Sensors with Mixed Aromatic Bicyclic Fused Rings and Pyridyl Groups: Solid Mediated Selective Detection of 2,4,6-Trinitrophenol in Water. ACS Omega, 3(3), 3248-3256.

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UV-Vis Absorbance Measurement of GtACR1

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Approximately 50 μg of reconstituted GtACR1 was washed in 300 mM NaCl/20 mM HEPES buffer (pH 7). To reduce the scattering, the solution was sonicated in three cycles of 30 s in an ice bath with a 30 s rest between cycles. The solution was placed in a quartz cuvette (Thorlabs, Inc., Newton, NJ), and a Cary 6000 spectrometer equipped with a diffuse scattering apparatus (Agilent Technologies, Inc., Santa Clara, CA) was used to perform the UV–vis absorbance measurement using a 0.1 s per step size of 1 nm (total scan time of 1 min). Light-adapted sample measurements were performed immediately after illumination for >5 min with a 530 nm LED (Thorlabs, Inc.) operating at approximately 10 mW/cm² at the sample. Dark-adapted sample measurements were performed after the sample had been kept in the dark for >30 min. To obtain the final reported spectra, baseline correction was performed by curve fitting to a combination of Rayleigh and Tyndall scattering curves to remove the scattering curve.

Yi A., Mamaeva N., Li H., Spudich J.L, & Rothschild K.J. (2016). Resonance Raman Study of an Anion Channelrhodopsin: Effects of Mutations near the Retinylidene Schiff Base. Biochemistry, 55(16), 2371-2380.

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