Puerarin

Manufactured by Chengdu Must Bio-Technology

Sourced in China

Puerarin is a bioactive compound extracted from the root of the Pueraria lobata plant. It serves as a key raw material for various pharmaceutical and nutraceutical applications. The compound exhibits a range of physiological properties that are of interest to researchers and product developers.

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Lab products found in correlation

7 protocols using puerarin

Procurement of Bioactive Compounds

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Puerarin (≥99.71%), 3-CQA (≥99.37%), 4-CQA (≥99.39%), 5-CQA (≥99.07%) and EGCG were purchased from Chengdu Must Biotechnology Co., Ltd (Chengdu, China). Methanol and acetonitrile for HPLC were obtained from Scharlau Co., Ltd (Barcelona, Spain). AA was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All other chemicals were of analytical grade and obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).

Wang D., Liu J., Qiu S., Wang J., Song G., Chu B., Li L., Xiao G., Gong J, & Zheng F. (2021). Ultrasonic degradation kinetics and isomerization of 3- and 4-O-caffeoylquinic acid at various pH: The protective effects of ascorbic acid and epigallocatechin gallate on their stability. Ultrasonics Sonochemistry, 80, 105812.

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HPLC Analysis of Pueraria lobata Phytochemicals

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PLR was purchased from Nanjing Shang Yuan Tang Medicinal Store (Nanjing, China). Analytical grade methanol, potassium dihydrogen phosphate and dipotassium hydrogen phosphate were purchased from Nanjing Chemical Reagent Factory (Nanjing, China). HPLC-grade acetonitrile was purchased from Tedia (Fairfield, OH, USA). Analytical grade dimethyl sulfoxide (DMSO) was purchased from Shanghai Chemical Reagent Factory. The reference standards puerarin, daidzin and genistin were purchased from Cheng Du Must Biotechnology Co., Ltd. (purity >98%; Chengdu, China). Mirificin was purchased from Nanjing Spring & Autumn Biological Engineering Co., Ltd (purity >98%; Nanjing, China). Koic acid was purchased from Shanghai Yuanye biological technology Co., Ltd. (purity >98%; Shanghai, China). TYR and l-tyrosine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was prepared using a Millipore water purification system (Millipore, Bedford, MA, USA).

Liu H., Zhu Y., Wang T., Qi J, & Liu X. (2018). Enzyme-Site Blocking Combined with Optimization of Molecular Docking for Efficient Discovery of Potential Tyrosinase Specific Inhibitors from Puerariae lobatae Radix. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(10), 2612.

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Comprehensive Analysis of Fructus Puerariae

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A total of 13 batches of FP were collected from Zhejiang (S1), Guangdong (S2 and S3), Guangxi (S4, S5 and S6), Hebei (S8), Hunan (S9, S10, S11 and S12) and Sichuan (S13) provinces of China. Besides, the control herbs (S7) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Reference standards (purity >98%) including rutin, daidzin, daidzein, and quercetin were obtained from the National Institutes for Food and Drug Control (Beijing, China). Glycitin, genistin, tectoridin, glycitein, genistein, tectorigenin, and biochanin A with the purities over 98% were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Nicotiflorin and ononin with the purities over 98% were bought from Chengdu Alfa Biotechnology Co., Ltd. (Chengdu, China). Meanwhile, formononetin and puerarin were bought from Chengdu Must Bio-Technology Co., Ltd.(Chengdu, China). HPLC grade acetonitrile (ACN) and methanol (MeOH) were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid of HPLC grade was provided by Kermel Chemical Reagent Co., Ltd. (Tianjin, China) and pure water was obtained from Wahaha (Hangzhou, China). All the other reagents were of analytical grade.

Han J., Xu K., Yan Q., Sui W., Zhang H., Wang S., Zhang Z., Wei Z, & Han F. (2021). Qualitative and quantitative evaluation of Flos Puerariae by using chemical fingerprint in combination with chemometrics method. Journal of Pharmaceutical Analysis, 12(3), 489-499.

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Quantification of Dietary Antioxidants

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The standard of three diCQA isomers (3,5-, 4,5-, and 3,4-diCQA) (purity ≥ 99%), puerarin (purity ≥ 99.71%) and EGCG (purity ≥ 99.78%) were purchased from Chengdu Must Biotechnology Co., Ltd. (Chengdu, China). Ascorbic acid (VC) was purchases from Shanghai Aladdin Co., Ltd. (Shanghai, China). Terephthalic acid (TPA) and 2-hydroxyterephthalic acid (TPA-OH) were obtained from Sigma-Aldrich Chemical Co., Ltd. (Shanghai, China). Chromatographic grade acetonitrile and methanol were purchased from Merck KGaA company (Darmstadt, Germany). All other reagents used were of analytical grade or purer.

Wang D., Wang Y., Zhang Z., Qiu S., Yuan Y., Song G., Li L., Yuan T, & Gong J. (2023). Degradation, isomerization and stabilization of three dicaffeoylquinic acids under ultrasonic treatment at different pH. Ultrasonics Sonochemistry, 95, 106401.

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Quantitative Analysis of 5-CQA, Puerarin, and EGCG

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The standard of 5-CQA (purity ≥ 99.39%), puerarin (purity ≥ 99.71%) and EGCG (purity ≥ 99.78%) were purchased from Chengdu Must Biotechnology Co., Ltd. (Chengdu, China). Ascorbic acid was purchases from Shanghai Aladdin Co., Ltd. (Shanghai, China). Phosphoric acid solution (analytical grade) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic grade acetonitrile and methanol were purchased from Scharlau chromatographic reagent company (Barcelona, Spain).

Wang D., Wang J., Sun J., Qiu S., Chu B., Fang R., Li L., Gong J, & Zheng F. (2021). Degradation kinetics and isomerization of 5-O-caffeoylquinic acid under ultrasound: Influence of epigallocatechin gallate and vitamin C. Food Chemistry: X, 12, 100147.

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Antioxidant Pathway Regulation in Medicinal Slices

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PLR in the form of medicinal slices ready for decoction was obtained from Guangzhou Weida Company (Guangzhou, China). Puerarin (purity ≥ 99.0%), daidzin (purity ≥ 98.0%), and daidzein (purity ≥ 98.0%) were obtained from the Chengdu Must Bio-Technology Company (Chengdu, Sichuan, China). Rabbit anti-Nrf2 antibody (A1244), rabbit anti-HO-1 antibody (A1346), and rabbit anti-NQO1 antibody (A19586) were provided by ABclonal (Wuhan, China). Rabbit anti-Claudin-1 antibody (YT0942) was provided by ImmunoWay (Plano, TX, USA). Rabbit anti-Occludin antibody (ab216327) and rabbit anti-ZO-1 antibody (ab216880) were provided by Abcam (Cambridge, MA, USA). Mouse anti-β-actin antibody (RM2001) was provided by Beijing Ray Antibody Biotech (Beijing, China). Formaldehyde was obtained from Tianjin Da Mao chemical reagents factory (Tianjin, China).

Huang L., Liu Z., Wu P., Yue X., Lian Z., He P., Liu Y., Zhou R, & Zhao J. (2022). Puerariae lobatae Radix Alleviates Pre-Eclampsia by Remodeling Gut Microbiota and Protecting the Gut and Placental Barriers. Nutrients, 14(23), 5025.

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Puerarin Modulates Antioxidant Pathways

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Injectable Puerarin was purchased from Zhejiang Zhenyuan Pharmaceutical Co., Ltd. (Shaoxing, China). Puerarin was purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). Angiotensin II was purchased from Sigma (St. Louis, MO, United States). Tert-Butylhydroquinone (tBHQ) was purchased from MedChem Express (Monmouth Junction, NJ, United StatesA). Brusatol was purchased from Tautobiotech (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). The NE-PER nuclear and cytoplasmic extraction kit, Pierce BCA Protein Assay Kit, Pierce ECL Western Blotting Substrate and Chromatin Immunoprecipitation Kit (EZ-ChIP) were purchased from Thermo Fisher (Waltham, MA, United States). RNAiso Plus, PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) and SYBR Premix Ex Taq II (Tli RNaseH Plus) were purchased from TaKaRa (Shiga, Japan). Reactive Oxygen Species Assay Kit was purchased from Beyotime Institute of Biotechnology (Haimen, China).

Cai S.A., Hou N., Zhao G.J., Liu X.W., He Y.Y., Liu H.L., Hua Y.Q., Li L.R., Huang Y., Ou C.W., Luo C.F, & Chen M.S. (2018). Nrf2 Is a Key Regulator on Puerarin Preventing Cardiac Fibrosis and Upregulating Metabolic Enzymes UGT1A1 in Rats. Frontiers in Pharmacology, 9, 540.

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