Wl03186

Male C57BL6/J mice weighing 20–25 g were housed at the Ethics Committee for Animal Experiments of the First Hospital of China Medical University under a 12 h:12 h light/dark cycle with a constant temperature (22 ± 1 °C) and free access to food and water. All animal experiments were performed according to the National Institutes of Health guide for the care and use of Laboratory animals, and were approved by the Animal Care and Use Committee of the First Hospital of China Medical University.
The detailed information of antibodies are as follows: Anti-ILEI antibody (ab72182, Abcam, Cambridge, U.K.), Anti-α‐SMA antibody (ab5694, Abcam), Anti-vimentin antibody (ab137321, Abcam), Anti-E‐cadherin antibody (ab133597, Abcam), Anti-p‐Akt antibody (ab18206, Abcam), Anti-Akt antibody (ab126811, Abcam), Anti-p‐ERK antibody (ab223500, Abcam), Anti- ERK antibody (ab17942, Abcam), Anti-collagen Ι antibody (ab34710, Abcam), Anti-LIFR antibody (sc-659, Santa cruz, Dallas, U.S.A.),Anti-collagen ΙII antibody (WL03186,wanleibio,Shenyang, China), Anti-β‐actin antibody (WL01845,wanleibio).

Doxorubicin hydrochloride (D1515) and berberine hemisulfate salt (B3412) were purchased from Sigma Aldrich (St. Louis, MO, USA). Trigonelline (HY-N0414) was obtained from MedChemExpress (Monmouth Junction, NJ, USA). Anti-α-SMA (ab7817), anti-Nrf2 (ab137550), anti-TFAM (ab131607), and anti-voltage-dependent anion channel (VDAC, ab15895) antibodies were obtained from Abcam (Cambridge, UK). Antibodies against vimentin (#5741), lamin B (#13435) and GAPDH (#2118) were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Antibodies against collagen I (WL0088) and collagen III (WL03186) were obtained from Wanleibio (Shenyang, Liaoning, China). Alexa Fluor 647-labeled goat anti-rabbit IgG (A32733), Alexa Fluor 488-labeled goat anti-mouse IgG (A11001), goat anti-rabbit IgG (#31462) and goat anti-mouse IgG (#31438) were obtained from Thermo Fisher Scientific (Logan, UT, USA). Reactive oxygen species (ROS, S0033S), malondialdehyde (MDA, S0131S) and superoxide dismutase (SOD, S0101S) assay kits were purchased from Beyotime Biotechnology (Nantong, China).