Abbv744

Tissues obtained from surgery were cut into pieces approximately 1 mm × 1 mm after removal of adipose tissues and blood vessels, and placed in T25 flasks. About 2 mL DMEM-F12 containing 20% fetal bovine serum (FBS) and 0.1% penicillin and streptomycin (both from Gibco Laboratories, Grand Island, NY, USA) was added and incubated at 37°C in a 5% CO2 humidified incubator. The media were changed every 3 days, and primary OFs were harvested after cells grew to confluence and were digested with a 0.25% trypsin solution (Gibco Laboratories). The cells were then sub-cultured in 6 cm plastic culture plates in DMEM-F12 with antibiotics and 10% FBS. OFs between the third and eighth passages were used for the subsequent experiments. Each part of the following experiment was repeated in OFs from at least three consecutive and independent specimens.
TGF-β1 (PeproTech Inc., Cranbury, NJ, USA), thiostrepton, BI6727, JQ1, and ABBV744 (all from Selleck Chemicals, Houston, TX, USA) were used for cell treatment. The solvent of thiostrepton, BI6727, JQ1, and ABBV744 was dimethylsulfoxide (DMSO; MP Biomedicals, Irvine, CA, USA). Control experiments were performed using DMSO as vehicle only without the addition of drugs.