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Tritest cd4 cd8 cd3

Manufactured by BD
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The BD Tritest CD4/CD8/CD3 is a laboratory equipment product that is used for the analysis and enumeration of T-lymphocyte subsets, specifically CD4+ T cells, CD8+ T cells, and CD3+ T cells. It provides a standardized and reliable method for the quantification of these important immune cell populations.

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Lab products found in correlation

Cobas taqman hiv 1 test version 2, by Roche (1 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

BD Tritest CD4 FITC/CD8 PE/CD3 PerCP Reagent TRITEST three-color CD4/CD8/CD3 reagent BD Tritest CD4/CD8/CD3 (FITC/PE/PerCP, TriTEST anti-CD4-FITC-CD8-PE-CD3-PerCP-reagents

4 protocols using tritest cd4 cd8 cd3

1

Measurement of HIV viral load and CD4+ cells

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HIV plasma viral load was determined with a commercial kit for nucleic acid amplification (COBAS TaqMan HIV-1 Test Version 2.0, Roche Molecular Systems, Branchburg, NJ, USA) with a linear range between 34 and 106 copies HIV RNA/mL.
CD4+ cell numbers were assessed by flow cytometry (BD Tritest CD4/CD8/CD3, Becton Dickinson, San Jose, CA, USA).
Rosca A., Anton G., Ene L., Iancu I., Temereanca A., Achim C.L, & Ruta S.M. (2016). Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART. Journal of immunoassay & immunochemistry, 38(3), 299-307.
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2

Quantifying CD4 Cell Counts by Flow Cytometry

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The CD4 cell number was determined using a flow cytometry-based assay (BD Tritest CD4/CD8/CD3, Becton Dickinson, San Jose, CA, USA).
Sultana C., Casian M., Oprea C., Ianache I., Grancea C., Chiriac D, & Ruta S. (2022). Hepatitis B Virus Genotypes and Antiviral Resistance Mutations in Romanian HIV-HBV Co-Infected Patients. Medicina, 58(4), 531.
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3

T Cell Enumeration by Flow Cytometry

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CD4+ and CD8+ T lymphocyte counts were performed by flow cytometry (BD Tritest CD4/CD8/CD3, Becton Dickinson, San Jose, CA).
Rosca A., Anton G., Botezatu A., Temereanca A., Ene L., Achim C, & Ruta S. (2016). miR-29a Associates With Viro-Immunological Markers of HIV Infection in Treatment Experienced Patients. Journal of medical virology, 88(12), 2132-2137.
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4

Flow Cytometric Analysis of T Cell Subsets

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To a 5-mL FalconTM polystyrene tube (Becton Dickinson and Company, Franklin Lakes, NJ, USA), fifty microliters of EDTA blood was added per 10 µL of BD Tritest CD4/CD8/CD3 (Becton Dickinson and Company, Franklin Lakes, NJ, USA). The method followed the manufacturer’s manual. The negative control tube was reacted with the BD FastImmuneTM γ1 PE/CD45 PerCP control (Becton Dickinson and Company, Franklin Lakes, NJ, USA). Samples were analyzed by BD FACSCaliburTM flow cytometry (Becton Dickinson and Company, Franklin Lakes, NJ, USA). Cytotoxic T (Tc) cells are CD3+CD8+, while helper T (Th) cells are CD3+CD4+. The data were analyzed by BD CellQuest software version 5.0, 2002 (Becton Dickinson and Company, Franklin Lakes, NJ, USA).
Pukanha K., Yimthiang S, & Kwanhian W. (2020). The Immunotoxicity of Chronic Exposure to High Levels of Lead: An Ex Vivo Investigation. Toxics, 8(3), 56.
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