dLNs were collected and single-cell suspensions were prepared, counted, stained with FITC-anti-CD11c (clone N418, eBiociences, dilution 1:100), APC-anti-CD40 (clone 3/23, Biolegend, dilution 1:100) and PerCp-Cy5.5-anti-CD86 (clone GL-1, Biolegend, dilution 1:100) antibodies, followed by flow cytometry. Total numbers of CD11c+cells, CD40+CD11c+ cells or CD86+ CD11c+ cells per lymph node were calculated in the basis of the total number of cells and percentages of each cell subset in the lymph node.
Apc anti cd40 clone 3 23
Manufactured by BioLegend
APC-anti-CD40 (clone 3/23) is a fluorochrome-conjugated antibody that binds to the CD40 surface receptor. CD40 is a co-stimulatory molecule expressed on various immune cells, including B cells, dendritic cells, and macrophages. This antibody can be used for the identification and enumeration of CD40-positive cells in flow cytometry applications.
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Lab products found in correlation
2 protocols using apc anti cd40 clone 3 23
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Quantifying Dendritic Cell Activation
2
Quantifying Dendritic Cell Activation
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dLNs were collected and single-cell suspensions were prepared, counted, stained with FITC-anti-CD11c (clone N418, eBiociences, dilution 1:100), APC-anti-CD40 (clone 3/23, Biolegend, dilution 1:100) and PerCp-Cy5.5-anti-CD86 (clone GL-1, Biolegend, dilution 1:100) antibodies, followed by flow cytometry. Total numbers of CD11c+cells, CD40+CD11c+ cells or CD86+ CD11c+ cells per lymph node were calculated in the basis of the total number of cells and percentages of each cell subset in the lymph node.
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