NHS-functionalized PLGA (PLGA-NHS) (120 mg, 1.5 × 10−5 mol) dissolved in 8 mL of dimethyl sulfoxide (DMSO) and aminated HA (150 mg, 1 × 10−5 mol) were transferred into the reaction vial. N,N-Diisopropy-lethylamine (4.4 μL, 2.5 × 10−5 mol) was then added dropwise under stirring. After reaction for 48 h at 50 °C under stirring, the mixture was then purified by dialysis (Fisherbrand Regenerated Cellulose Dialysis Tubing, MWCO: 12−14 kDa) against DI water for 72 h. The final product was harvested and lyophilized to give a white solid. PLGA-HA-RGD and PLGA-HA-cRGD were also synthesized using the same protocol but with the masses of reagents scaled accordingly.
Regenerated cellulose dialysis tubing
Manufactured by Thermo Fisher Scientific
Sourced in United States
Regenerated Cellulose Dialysis Tubing is a semi-permeable membrane used in dialysis processes. It allows the passage of small molecules while retaining larger molecules, proteins, and other macromolecules. The tubing is made from regenerated cellulose and is designed for applications that require size-based separation of components in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Astm type 2 deionized water, by Avantor (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Isopropyl alcohol, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Levonorgestrel lng, by Merck Group (1 mentions)
Poly ε caprolactone pcl, by Merck Group (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Styrene, by Thermo Fisher Scientific (1 mentions)
2 2 azobis 2 methylpropionamidine dihydrochloride, by Thermo Fisher Scientific (1 mentions)
Pegma, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
N n dimethylformamide (dmf), by Thermo Fisher Scientific (1 mentions)
Iron 3 chloride hexahydrate, by Thermo Fisher Scientific (1 mentions)
Iron 2 chloride hexahydrate, by Thermo Fisher Scientific (1 mentions)
2 aminoethyl phosphonic acid, by Merck Group (1 mentions)
7 protocols using regenerated cellulose dialysis tubing
1
Synthesis of PLGA-HA Conjugates
2
Cellulose Extraction from Wood Pulp
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Bleached wood pulp (W-50 grade) was provided by Nippon Paper Chemicals Co., Ltd. (Tokyo, Japan). Sulfuric acid (98%, EMD Millipore Corporation, Chicago, IL, USA) was diluted to a concentration of 48 wt % before use. Type H well cement (Halliburton Energy Services, Houston, TX, USA). ASTM Type II deionized water with a resistivity of 10 MΩ cm was purchased from VWR International LLC (Radnor, PA, USA). Regenerated cellulose dialysis tubing was purchased from Fisher Scientific (Pittsburgh, PA, USA).
3
Doxorubicin Release from Micelle Formulation
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Doxorubicin-loaded micelle solution (2 mL), with a concentration of 1 mg/mL, was placed in a dialysis membrane (Fisherbrand Regenerated Cellulose Dialysis Tubing, 12,000 MWCO). The dialysis membrane was placed into 0.05% SDS, containing acetate buffer (10 mM, 150 mM NaCl, pH 5) or PBS (10 mM, pH 7.4) buffer, and shaken (100 rpm) at 37 °C. At predetermined time intervals, 1 mL of buffer solution was withdrawn and replaced with fresh buffer. DOX release was determined by measuring the absorption (at 496 nm) of the DOX molecule in withdrawn buffers, and the cumulative release plots were obtained using the formula below.
According to this, Vm: emission media volume; W0 (mg): the amount of drug loaded; CDOX (n): the amount of DOX (mg/mL) in the sample taken from the release medium; CDOX (n − 1): (n − 1). the amount of DOX in the sample taken from the media.
According to this, Vm: emission media volume; W0 (mg): the amount of drug loaded; CDOX (n): the amount of DOX (mg/mL) in the sample taken from the release medium; CDOX (n − 1): (n − 1). the amount of DOX in the sample taken from the media.
4
Levonorgestrel-Loaded PCL/PVA Nanoparticles
5
Synthesis of Gold Nanoparticles
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Styrene (S; 99% purity), 2VP (97% purity),
and 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AIBA;
98% purity) were acquired from Acros Organics. PEGMA (average Mn = 2000, 50 wt % in H2O) and DVB
(55% purity) were purchased from Sigma-Aldrich. N-Methyl-N,N,N-trioctyloctan-1-ammonium
chloride (Aliquat 336) was used as received from Alfa Aesar. Potassium
tetrachloroaurate (KAuCl4), hydrogen tetrachloroaurate
(HAuCl4), sodium borohydride (NaBH4), silver
nitrate (AgNO3), and dry alumina oxide were obtained through
Fischer Scientific, Inc. Hydrochloric acid (HCl) was used as received
from Macron Fine Chemicals. Ultrapure water was produced using a Barnstead
Nanopure water purifier and had an overall resistivity of 18 MΩ·cm.
Regenerated cellulose dialysis tubing with a MWCO of 8000 or 12 000–14 000
were used as received from Fisher Scientific, Inc. The purification
of S, 2VP, and DVB was done under Schlenk conditions by passing each
monomer through dry alumina oxide powder to remove the polymerization
inhibitors. The purified monomers were used within 24 h, and if short-term
storage was required, they were sealed in round bottom flasks with
an inert atmosphere and stored in the dark at −18 °C.
and 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AIBA;
98% purity) were acquired from Acros Organics. PEGMA (average Mn = 2000, 50 wt % in H2O) and DVB
(55% purity) were purchased from Sigma-Aldrich. N-Methyl-N,N,N-trioctyloctan-1-ammonium
chloride (Aliquat 336) was used as received from Alfa Aesar. Potassium
tetrachloroaurate (KAuCl4), hydrogen tetrachloroaurate
(HAuCl4), sodium borohydride (NaBH4), silver
nitrate (AgNO3), and dry alumina oxide were obtained through
Fischer Scientific, Inc. Hydrochloric acid (HCl) was used as received
from Macron Fine Chemicals. Ultrapure water was produced using a Barnstead
Nanopure water purifier and had an overall resistivity of 18 MΩ·cm.
Regenerated cellulose dialysis tubing with a MWCO of 8000 or 12 000–14 000
were used as received from Fisher Scientific, Inc. The purification
of S, 2VP, and DVB was done under Schlenk conditions by passing each
monomer through dry alumina oxide powder to remove the polymerization
inhibitors. The purified monomers were used within 24 h, and if short-term
storage was required, they were sealed in round bottom flasks with
an inert atmosphere and stored in the dark at −18 °C.
6
Ketorolac Release from Sol-Gel Silica Preparations
7
Functionalized PEG Polymers for Biomedical Applications
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Thiol-poly (ethylene glycol)-N-Hydroxysuccinimide and thiol-poly (ethylene glycol)-nitrilotriacetic acid (MW 3400 Da) were purchased from Biochempeg Scientific, Inc. (Watertown, MA). 2-aminoethylphosphonic acid and dopamaine hydrochloride were purchased from Sigma Aldrich, Inc. (St. Louis, MO). All organic solvents [N-methylmorpholine (99%), 200-proof ethanol (99.5%, ACS), acetone (ACS), isopropanol (ACS), tetraethylammonium hydroxide (10% in water), and N,N-dimethylformamide (99.8%)], iron (III) chloride hexahydrate, iron (II) chloride hexahydrate, regenerated cellulose dialysis tubing [6,000–8,000 Da, and 3,500 Da Molecular Weight Cut-Off (MWCO)], HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] buffer (1 M, pH 8), and hydrochloric acid were purchased from Fisher Scientific.
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