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Dnaamp kit

Manufactured by Qiagen
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The DNAamp kit is a laboratory instrument designed for the amplification of DNA samples. It provides a reliable and efficient method to increase the quantity of a specific DNA sequence from a small initial sample.

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Lab products found in correlation

Hiseq 2000, by Illumina (2 mentions) Snp 6.0 array, by Thermo Fisher Scientific (2 mentions)

2 protocols using dnaamp kit

1

Genomic DNA Sequencing and Variant Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was isolated from frozen tissues using the Qiagen DNAamp kit or a standard proteinase K protocol. Samples were sequenced on an Illumina HiSeq-2000 to an average of 77× depth (85.6% targeted reads > 20× coverage). The average rate of read alignment was 98.6%. Affymetrix SNP 6.0 arrays were used for a subset of samples. Seven CAHs, out of 19 profiled, were found to have both an exceptionally low purity (less than 25% per ABSOLUTE analysis23 ) and low burden of mutations. Upon manual review, the mutations whose allelic fractions were higher than 10% were enriched in regions with low mapping quality. These seven samples were therefore excluded from further analyses.
Gibson W.J., Hoivik E.A., Halle M.K., Taylor-Weiner A., Cherniack A.D., Berg A., Holst F., Zack T.I., Werner H.M., Staby K.M., Rosenberg M., Stefansson I.M., Kusonmano K., Chevalier A., Mauland K.K., Trovik J., Krakstad C., Giannakis M., Hodis E., Woie K., Bjorge L., Vintermyr O.K., Wala J.A., Lawrence M.S., Getz G., Carter S.L., Beroukhim R, & Salvesen H.B. (2016). The genomic landscape and evolution of endometrial carcinoma progression and abdominopelvic metastasis. Nature genetics, 48(8), 848-855.
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2

Genomic DNA Sequencing and Variant Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was isolated from frozen tissues using the Qiagen DNAamp kit or a standard proteinase K protocol. Samples were sequenced on an Illumina HiSeq-2000 to an average of 77× depth (85.6% targeted reads > 20× coverage). The average rate of read alignment was 98.6%. Affymetrix SNP 6.0 arrays were used for a subset of samples. Seven CAHs, out of 19 profiled, were found to have both an exceptionally low purity (less than 25% per ABSOLUTE analysis23 ) and low burden of mutations. Upon manual review, the mutations whose allelic fractions were higher than 10% were enriched in regions with low mapping quality. These seven samples were therefore excluded from further analyses.
Gibson W.J., Hoivik E.A., Halle M.K., Taylor-Weiner A., Cherniack A.D., Berg A., Holst F., Zack T.I., Werner H.M., Staby K.M., Rosenberg M., Stefansson I.M., Kusonmano K., Chevalier A., Mauland K.K., Trovik J., Krakstad C., Giannakis M., Hodis E., Woie K., Bjorge L., Vintermyr O.K., Wala J.A., Lawrence M.S., Getz G., Carter S.L., Beroukhim R, & Salvesen H.B. (2016). The genomic landscape and evolution of endometrial carcinoma progression and abdominopelvic metastasis. Nature genetics, 48(8), 848-855.
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