HEK-293 cells were divided into the miR-335-5p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO- MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5ʹ-cATTTAACTTCTAGTTGCTCTTGCc-3ʹ and down 5ʹ-tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3ʹ; and mutant MAPK10, up 5ʹ-cATTTAACTTCTAGTTGATATCGCc-3ʹ and down 5ʹ-tcgagGCGATATCAACTAGAAGTTAAATgagct-3ʹ. Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
Wild type mapk10
Manufactured by Genechem
Sourced in China
Wild-type MAPK10 is a recombinant protein that corresponds to the full-length sequence of the mitogen-activated protein kinase 10 (MAPK10) enzyme. MAPK10 is a member of the MAPK family and plays a role in various cellular processes.
Automatically generated - may contain errors
2 protocols using wild type mapk10
1
Investigating miR-335-5p and MAPK10 Interaction
2
Luciferase Assay of MAPK10 Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HEK-293 cells were divided into the miR-30a-3p and pmirGLO empty vector, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Cells without treatment were used as control. The MAPK10 wild-type and mutant fragments were synthesized by Genechem, Shanghai, China, as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . These cells were inoculated onto the 96-well plates, and cultured for 24 h. The luciferase activity was detected by the microplate reader. Renilla was used as internal reference.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!