Sulphadiazine

Manufactured by Merck Group

Sourced in United States

Sulphadiazine is a laboratory reagent used for various analytical and research applications. It is a white crystalline powder that acts as a sulfonamide antimicrobial agent. Sulphadiazine is commonly used in the pharmaceutical and biomedical industries for its chemical and biological properties, but its specific intended use should not be extrapolated beyond its core function as a laboratory reagent.

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Lab products found in correlation

2 protocols using sulphadiazine

Comprehensive Veterinary Drug Extraction

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All solvents (HPLC/analytical grade) were purchased from Fluka (Sigma- Aldrich, St. Louis, MO, USA). Trichloroacetic acid (TCA) crystals and the reagents to prepare an EDTA-McIlvaine buffer solution, pH 4 (disodium hydrogen phosphate dihydrate, citric acid monohydrate and EDTA) were purchased from Fluka.
Formic acid (98–100%) was obtained from Riedel-de Haën (Sigma-Aldrich, St. Louis, MO, USA).
The extraction cartridges (Oasis HLB 3 mL, 60 mg) were provided by Waters (Milford, MA, USA).Amoxicillin, ampicillin, benzylpenicillin, cefalexin, cefquinome, ceftiofur, chloramphenicol, chlortetracycline, ciprofloxacin, danofloxacin, dimetridazole, doxycycline, enrofloxacin, florfenicol, florfenicol amine, flumequine, furaltadone, furazolidone, lincomycin, lomefloxacin hydrochloride, marbofloxacin, nalidixic acid, nitrofurazone, oxolinic acid, oxytetracycline, ronidazole, spyramicin, sulphadiazine, sulphadimethoxine, sulphadimidine, sulphamerazine, sulphathiazole, tetracycline hydrochloride, thiamphenicol, tilmicosine, tinidazole, trimethoprim, tylosin, and enrofloxacin d5 as the internal standards (IS) (purity >98%) were used and purchased from Fluka (Sigma-Aldrich, St. Louis, MO, USA).

Savarino A.E., Terio V., Barrasso R., Ceci E., Panseri S., Chiesa L.M, & Bonerba E. (2020). Occurrence of antibiotic residues in Apulian honey: potential risk of environmental pollution by antibiotics. Italian Journal of Food Safety, 9(1), 8678.

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Enumeration of Antibiotic-Resistant Bacteria in Lettuce

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The isolation and enumeration of tetracycline-resistant and sulphadiazine-resistant bacteria in lettuce samples was performed using a method modified from methods described by Marti et al. and Esiobu et al. [19 (link),20 (link)]. Briefly, lettuce leaves were cut into small pieces using autoclaved scissors. Fifty grams of the samples were homogenised in a sterile stomacher bag with 100 mL autoclaved water at 200 rpm for 2 min. The homogenates were diluted with autoclaved deionised water by tenfold. The undiluted and diluted homogenate were added to Mueller–Hinton agar (Sigma-Aldrich, St. Louis, MO, USA) containing 4 µg/mL tetracycline (Sigma-Aldrich, St. Louis, MO, USA) and Mueller–Hinton agar containing 1024 µg/mL sulphadiazine (Sigma-Aldrich, St. Louis, MO, USA) in triplicate and incubated at 37 °C for 48 h.

Lam K.L., Kong W.P., Ling P.Y., Lau T.H., Ho K.H., Lee F.W, & Chan P.L. (2020). Antibiotic-Resistant Bacteria in Hydroponic Lettuce in Retail: A Comparative Survey. Foods, 9(9), 1327.

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