Ammonium tetrathiomolybdate (Sigma-Aldrich); hydrazine hydrate (N2H4, Merck); sodium borohydride (NaBH4, Merck); tellurium powder (Te, Sigma-Aldrich); cadmium chloride hemipentahydrate (CdCl2·2.5H2O); hydrochloric acid (HCl, Merck); cysteamine (Sigma-Aldrich), PUR-A-LYZER MEGA DIALYSIS KIT (Sigma-Aldrich); Milli-Q water.
Pur a lyzer mega dialysis kit
Manufactured by Merck Group
Sourced in United States
The PUR-A-LYZER MEGA DIALYSIS KIT is a laboratory equipment product designed for dialysis applications. It facilitates the removal of small molecules from samples through the process of dialysis.
Automatically generated - may contain errors
Lab products found in correlation
Cadmium chloride hemi pentahydrate, by Merck Group (1 mentions)
Ammonium tetrathiomolybdate, by Merck Group (1 mentions)
Tellurium powder te, by Merck Group (1 mentions)
Hydrazine hydrate n2h4, by Merck Group (1 mentions)
Sodium borohydride nabh4, by Merck Group (1 mentions)
Cysteamine, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
2 protocols using pur a lyzer mega dialysis kit
1
Synthesis of Tellurium Nanoparticles
2
Inactivation of Commercial Enzymes
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Solutions of commercial enzymes (25% v/v) were prepared in 50 mM phosphate buffer at pH 7. The solutions were then dialysed overnight against the same buffer using a Pur-A-Lyzer™ Mega Dialysis Kit (Sigma-Aldrich, St. Louis, USA). After dialysis, half of the enzyme solution was taken to prepare the inactive enzyme solution by placing it in an oven at 103 °C for 6 h followed by immediate exposure to −20 °C for at least 2 h. This temperature shock resulted in the irreversible inactivation of the enzyme as verified in the enzymatic assays described in section 2.4.
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