Bltr1 antibody

Recombinant human HMGB1 antibody was purchased from R&D System Inc. (Minneapolis, MN, USA). Zileuton (( ±)-N-hydroxy-N-(1-benzo[b]thien-2-ylethyl)urea) was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). The MCP-1 antibody was purchased from Invitrogen (Carlsbad, CA, USA) and BLTR1 antibody was purchased from Biorbyt (Cambridge, UK). 5-LO and β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Beverly, MA, USA). LTB4, LTC4, BLTR1 inhibitor (U75302), BLTR2 inhibitor (LY255283), LTC4 receptor (CysLTR) 1 inhibitor (Montelukast) and CysLTR2 inhibitor (HAMI3379) were purchased from Cayman Chemical Inc. (Ann Arbor, MI, USA). Horseradish peroxidase (HRP)-conjugated IgG antibody (Santa Cruz Biotechnology Inc.) was used as secondary antibody.

Zileuton and alpha-smooth muscle actin (α-SMA) antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). LTB4, U75302 and REV5901 were from Cayman Chemical Inc (Ann Arbor, MI, USA). MK 886 was purchased from EMD Serono (Rockland, MA, USA), HMGB1 from R&D systems (Minneapolis, MN, USA), CD11b antibody from Abcam (Cambridge, MA, USA), and BLTR1 antibody from Biorbyt (Cambridge, UK). CD36, CD14, β-actin, and 5-LO antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), R-phycoerythrin PE-conjugated mouse anti-human CD11b/Mac-1 antibody and PE-conjugated mouse IgG isotype control antibody from BD (San Diego, CA, USA). Horseradish peroxidase (HRP)-conjugated IgG antibody was used as the secondary antibody from Santa Cruz Biotechnology. Restriction enzymes were purchased from Promega (Madison, WI, USA). 5-LO and BLTR1 siRNA oligonucleotides were synthesized by Bioneer (Daejeon, ROK). siRNA molecules were transfected into cells using Lipofectamine 2000 siRNA transfection reagent (Invitrogen, Carlsbad, CA, USA). PCR primers were from Bioneer.