Sephadex g 75

Potato dextrose water was purchased from Hope Bio-Technology Co., Ltd, Qingdao, China. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), phenol (PH, > 99%), 4-chlorophenol (CP, > 99%), and bisphenol A (BPA, > 99%) were acquired from Macklin Biochemical Co., Ltd, Shanghai, China. Filters of DEAE-Sepharose Fast Flow and Sephadex G-75 were purchased from Shanghai yuanye Bio-Technology Co., Ltd, Shanghai, China. All other chemical reagents used were analytical grade. Agricultural wastes (rice straw, wheat bran, sugarcane bagasse, pine sawdust, pomelo peel, navel orange peel, and rapeseed oil cake) were purchased from Taobao Mall, a Chinese online retail platform. These agro-wastes were dried and ground into powder (60 mesh).

Water extract of Radix Astragali was provided by Zhejiang Xinguang Pharmaceutical Co., Ltd. (Shengzhou, China). DEAE cellulose-52 and Sephadex G-75 were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Dextrans with different molecular weights were purchased from Waters Technologies (Milford, MA, USA). 1-Phenyl-3-methyl-5-pyrazolone (PMP), trifluoroacetic acid (TFA), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Shanghai Macklin Biochemistry Corporation (Shanghai, China). Standard monosaccharides (mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose) and ascorbic acid were purchased from Aladdin Reagents Co., Ltd. (Shanghai, China). The H9c2 cardiomyocytes were purchased from Cell Bank of Chinese Academy of Sciences and fetal bovine serum (FBS) was purchased from Hangzhou Sijiqing Co., Ltd. (Hangzhou, China). Cell Counting Kit-8 (CCK-8) and DMEM medium were purchased from Beijing Solarbio Sciences & Technology Co., Ltd. (Beijing, China). The Langendorff’s apparatus (LGF-2) was purchased from Shanghai Wolong Technology Co., Ltd. (Shanghai, China). Assay kits for LDH, CK, and AST were obtained from Nanjing Jiancheng Bioengineering Institute. All other reagents used in this study were of analytical grade.