Abx pentra

Manufactured by Horiba

Sourced in France

The ABX Pentra is a versatile hematology analyzer designed for clinical laboratories. It is capable of performing complete blood count (CBC) analysis, including the measurement of red blood cells, white blood cells, and platelets. The ABX Pentra is a reliable and efficient instrument for providing accurate and standardized blood analysis results.

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Lab products found in correlation

Cobas mira, by Roche (1 mentions) Optima l 80 xp, by Beckman Coulter (1 mentions) Abx pentra 400 analyzer, by Horiba (1 mentions) Mouse rat insulin kit, by Mesoscale (1 mentions) Trig gb, by Roche (1 mentions) Nefa hr 2, by Fujifilm (1 mentions) Abx pentra 400, by Horiba (1 mentions) Glucose hk cp, by Horiba (1 mentions) Abc vet, by Horiba (1 mentions) Humalog insulin, by Eli Lilly (1 mentions) Precision xtra, by Abbott (1 mentions) Triglyceride cp kit, by Horiba (1 mentions)

11 protocols using abx pentra

Blood Sampling and Analysis Protocol

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The blood samples were collected in an ethylenediamine tetra acetic acid EDTA tube from each participant on the days of the experimental trial under an aseptic technique. The blood was collected on nutritional trial day from each participant fasting at baseline on 0 min, 30 min after supplementation and after breakfast and lunch. Blood samples were centrifuged by 4000 rpm for 10–15 min in refrigerated centrifuge. The plasma supernatant was collected in Eppendorf tubes and stored at −80°C before analysis.
For insulin analysis the Abbott Architect c8000 system was used, utilizing potentiometric and photometric technology on immunoassay tests, utilizing CM1A (Chemiluminescent Microparticles assay). The glucose concentration was determined by enzymatic calorimetric method by using kits containing (glucose HK-CP reagent ABX Pentra, Horiba ABX, France) on the spectrophotometric analyser on automatic Roche Cobas Mira.

Mohammad N.S., Nazli R., Fatima S., Fozia F., Zafar H., Zafar M., Zafar Z., Khan W., Abulmeaty M.M., Aldisi D., Andrade Laborde J.E, & Aboul-Soud M.A. (2024). Lipid-based nutritional supplement impact on energy intake, appetite, glucose and insulin levels in under-weight pregnant and lactating women with preeclampsia. Bioscience Reports, 44(1), BSR20231344.

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Quantitative Lipoprotein Profiling by Ultracentrifugation

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Quantification of lipoprotein (LP) particles was performed using ultracentrifugation (UC) as previously described (1). Seven different lipoprotein molecules including cholesterol (chol), triglycerides (tg), cholesterol ester (chole), free cholesterol (fchol), phospholipids (phosl), apolipoprotein A (apoA) and apolipoprotein B (apoB) were quantified in all or in some of the LP main fractions (VLDL, IDL, HDL, LDL) and/or in their subfractions (HDL2a, HDL2b, HDL3, LDL1, LDL2, LDL3, LDL4, LDL5, LDL6). Fractionation was done by sequential centrifugation of 3 mL EDTA plasma using an Optima L-80 XP ultracentrifuge with a fixed angle rotor type 50.4
Ti (Beckman Coulter, Inc., US). The UC process was initiated immediately after the fasting plasma sample was collected, and it was completed over a period of 8 days. A detailed description of the separation steps can be found in (1). Immediately after the fractionation step, all subfractions were frozen and stored at -80⁰C for later analysis.
Colorimetric and turbidimetric assays were performed on an ABX Pentra 400 analyzer (ABX Pentra; Horiba ABX, Montpellier, France) to determine the plasma, main class and subclass concentrations of total chol, tg, apoA and apoB (ABX Pentra; Horiba Medical, France). Free cholesterol and phoslp were determined using colorimetric and turbidimetric assays (MTI Diagnostics, Germany).

Khakimov B., Hoefsloot H.C.J., Mobaraki N., Aru V., Kristensen M., Lind M.V., Holm L., Castro‐Mejía J.L., Nielsen D.S., Jacobs D.M., Smilde A.K., & Engelsen S.B. (2021). Human blood lipoprotein predictions from ¹H NMR spectra: protocol, model performances and cage of covariance.

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Insulin and Metabolic Biomarker Quantification

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Insulin was measured using a Mouse/Rat Insulin kit from Meso Scale Discovery (K152BZC-1). Enzymatic colorimetric methods, assayed on the ABX Pentra 400 (Horiba ABX), were used for analysis of: glucose (Glucose HK CP, A11A01667 Horiba ABX), TGs (Trig/GB, 11877771216, Roche Diagnostics GmbH), cholesterol (Cholesterol CP ABX Pentra, A11A01634, HORIBA ABX), β-hydroxybutyrate (RANBUT: D-3-hydroxybutyrate, RB1007, Randox) and free fatty acids (NEFA-HR[2] WaKO Chemicals GmbH). Liver tissue TG levels were assessed after homogenization of approximately 50 mg tissue in isopropanol followed by analysis of TG levels in the supernatant (TGs CP, ABX Pentra, A11A01640, Horiba ABX).

Bartesaghi S., Wallenius K., Hovdal D., Liljeblad M., Wallin S., Dekker N., Barlind L., Davies N., Seeliger F., Winzell M.S., Patel S., Theisen M., Brito L., Bergenhem N., Andersson S, & Peng X.R. (2022). Subcutaneous delivery of FGF21 mRNA therapy reverses obesity, insulin resistance, and hepatic steatosis in diet-induced obese mice. Molecular Therapy. Nucleic Acids, 28, 500-513.

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Multimodal Blood Analysis Protocol

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A whole blood sample was used for blood count analysis using an automatic haematology
analyser ABC Vet (Horiba, Kyoto, Japan). Plasma obtained after centrifugation
(acceleration: 1000 × g, run time: 10 min, 4°C) was used for measuring
the lipid profile with an ABX Pentra biochemical analyser (Horiba Medical Kyoto,
Japan).
Measurement of nitrate (

{NO}_{3}^{-}

) and nitrite (

{NO}_{2}^{-}

) concentrations in the plasma was performed using an
ENO-20 NOx analyser (Eicom Corp., Kyoto, Japan), applying a liquid chromatography method
with post-column derivatization using Griess reagent, as previously described.^{38 (link)} The packed RBCs remaining after
centrifugation were used for gluthatione (GSH) and glutathione disulfide (GSSG)
concentration measurement as well as nitrosyl haemoglobin (HbNO) detection with EPR
spectroscopy.^{38 (link),39 (link)}

Mohaissen T., Proniewski B., Targosz‐Korecka M., Bar A., Kij A., Bulat K., Wajda A., Blat A., Matyjaszczyk-Gwarda K., Grosicki M., Tworzydlo A., Sternak M., Wojnar-Lason K., Rodrigues-Diez R., Kubisiak A., Briones A., Marzec K.M, & Chlopicki S. (2021). Temporal relationship between systemic endothelial dysfunction and alterations in erythrocyte function in a murine model of chronic heart failure. Cardiovascular Research, 118(12), 2610-2624.

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Automated Lipid and Glucose Analysis

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Fasting blood glucose and lipid profiles (total cholesterol, high-density lipoproteins, low-density lipoprotein, and triglycerides) were determined by ABX Pentra automated analyzer (Horiba ABX SAS, Montpellier, France). Lipid profiles were measured by the enzymatic colorimetric test principle, and a series of coupled enzymatic reactions were carried out to form a colored chromogenic complex. The absorbance of the colored dye was measured at a fixed wavelength and is proportional to the concentration of the lipid profile in the sample. The enzymatic method was used to determine glucose, glucose was measured by the PAP-CP, enzymatic photometric method, and then finally absorbance was measured.

Haile K., Kedir R., Timerga A., Mose A, & Arkew M. (2022). Role of platelet indices as diagnostic and predictive biomarkers for comorbidity of diabetes and metabolic syndrome in southern Ethiopia: A comparative cross-sectional study. PLOS ONE, 17(11), e0277542.

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Diethylnitrosamine-induced Liver Injury

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Fourteen-day-old mice were injected intraperitoneally with 25 mg/kg of DEN (Sigma) [56 (link)]. Serum ALT and AST levels were determined using ABX Pentra (Horiba Medical).

Ferrara-Romeo I., Martínez P, & Blasco M.A. (2018). Mice lacking RAP1 show early onset and higher rates of DEN-induced hepatocellular carcinomas in female mice. PLoS ONE, 13(10), e0204909.

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Metabolic Biomarker Profiling in Mice

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Serum levels of albumin, creatinine, bilirubin, urea, alanine aminotransferase (ALT), cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) were determined using ABX Pentra (Horiba Medical). To perform GTT and ITT, mice were i.p. injected with 2 g of glucose/kg of body weight and 0.75 U insulin/kg of body weight (Eli Lilly; Humalog Insulin), respectively. In the case of GTT mice were previously fasted for 16 h. For ITT mice were not fasted and basal levels of glucose were between 80–100 mg/dL.

Muñoz-Lorente M.A., Cano-Martin A.C, & Blasco M.A. (2019). Mice with hyper-long telomeres show less metabolic aging and longer lifespans. Nature Communications, 10, 4723.

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Measurement of Plasma Biomarkers

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Blood BHB was measured by using a handheld monitor and reagent strips (Precision Xtra, Abbott Diabetes Care, Maidenhead, UK). Blood samples were stored on ice and centrifuged; duplicate plasma aliquots were stored at −80°C and analyzed within 6 weeks. Plasma glucose was assayed by using a commercial analyzer (ABX Pentra; HORIBA ABX SAS, Montpellier, France), while insulin and total ghrelin were measured by using commercially available enzyme‐linked immunosorbent assays (ELISA) (Mercodia AB, Uppsala, Sweden, and Merck, Millipore, Germany). Total glucagon‐like peptide 1 (GLP‐1) and peptide tyrosine tyrosine (PYY) assays were performed by the Core Biochemical Assay Laboratory of the National Institute for Health Research Cambridge Biomedical Research Centre (http://www.cuh.org.uk/core-biochemical-assay-laboratory).

Stubbs B.J., Cox P.J., Evans R.D., Cyranka M., Clarke K, & de Wet H. (2017). A Ketone Ester Drink Lowers Human Ghrelin and Appetite. Obesity (Silver Spring, Md.), 26(2), 269-273.

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Fasting Plasma Biomarker Analysis

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Prior to screening, participants were instructed to fast for 12 hours before the study visit. Venous blood was collected in EDTA tubes, centrifuged at 1500 g for 10 minutes to produce plasma, this was then frozen at −80°C for subsequent measurement of research biomarkers. Quantitative analysis of plasma for human CRP, ApoA1 and ApoB was carried out on the ABX Pentra clinical chemistry analyser and latex-enhanced immunoturbidimetric assay (Horiba medical, Northampton, UK). TNF-α and IL-6 were analysed using the Quantikine high sensitivity ELISA for human TNF-alpha /TNFSF1A and human IL-6 kit, (R&D Systems, UK). Leptin and resistin was assayed using the Mediagnost human enzyme-immunoassay ELISA kits (Reutlingen, Germany). Human adiponectin, insulin and 8-Iso prostaglandin F2 (8-Iso-PG F2) were assayed fluormetrically using the AutoDELFIA 1235 automatic immunoassay systems (Perkin Elma, Buckinghamshire UK). All samples were analysed in duplicate and with all duplicate samples having a CV% of ≤20%. All research biomarker assessments were carried out by University of Leicester Scientists in conjunction with Unilever personnel at the Unilever Corporate Research Laboratory, Bedford UK.

Webb M., Davies M., Ashra N., Bodicoat D., Brady E., Webb D., Moulton C., Ismail K, & Khunti K. (2017). The association between depressive symptoms and insulin resistance, inflammation and adiposity in men and women. PLoS ONE, 12(11), e0187448.

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Metabolic Biomarker Quantification Protocol

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Plasma TG, cholesterol, alanine transaminase (ALT) and aspartate transaminase (AST) concentrations were measured with commercial available kits (ABX Pentra, Horiba, Irvine, CA). Hepatic concentrations of TGs and cholesterol were measured using commercial available kits (ABX Pentra) after lipid extraction according to Bligh and Dyer (Bligh & Dyer 1959) (link). Plasma 3,3′,5-triiodothyronine (T3) concentrations were determined by radioimmunoassay as previously described (Friedrichsen et al. 2003) (link).

Grefhorst A., van den Beukel J.C., Dijk W., Steenbergen J., Voortman G.J., Leeuwenburgh S., Visser T.J., Kersten S., Friesema E.C.H., Themmen A.P.N, & Visser J.A. (2018). Multiple effects of cold exposure on livers of male mice. The Journal of endocrinology, 238(2).

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