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Pd 1 clone j43

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PD-1 (clone J43) is a monoclonal antibody that binds to the Programmed Cell Death Protein 1 (PD-1) receptor. PD-1 is an important immune checkpoint regulator that plays a role in the modulation of the immune response.

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Lab products found in correlation

Cd11b clone m1 70, by BD (2 mentions) Cd4 clone gk1, by BD (1 mentions) Lsrfortessa cytometry, by BD (1 mentions) B220 clone ra3 6b2, by BD (1 mentions) Cd19 clone 1d3, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Fixable near ir dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Icos clone c398.4a, by BioLegend (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Transcription factor buffer set, by BD (1 mentions) Tim 3, by BD (1 mentions) Cd8a clone 53 6, by BD (1 mentions) Melody cell sorter, by BD (1 mentions) Leukocyte activation cocktail, by BD (1 mentions) Ifn γ, by BD (1 mentions) Facsaria 3 sorter, by BD (1 mentions)

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PD-1 (J43) (4 citations) Hamster anti-mouse PD-1 clone J43, (2 citations)

4 protocols using pd 1 clone j43

1

Flow Cytometric Analyses of B Cells and Tfh Cells

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For flow cytometric analyses of B cell populations, single cells were first prepared from lymph nodes by grinding with sieve. After staining with fluorescein isothiocyanate-labeled antibodies for CD19 (clone 1D3, Cat. 557398, BD Biosciences), cells were counted with BD LSRFortessa (BD Biosciences) and analyzed using BD FACSDiva software (BD Biosciences) and FlowJo software (FlowJo, Ashland, OR, USA). For flow cytometric analyses of Tfh cells, lymphocytes were stained with antibodies to CD4 (clone GK1.5, Cat. 552051, BD Biosciences), B220 (clone RA3-6B2, Cat. 562290, BD Biosciences), CD11b (clone M1/70, Cat. 563015, BD Biosciences), PD-1 (clone J43, Cat. 562584, BD Biosciences), CXCR5 (clone 2G8, Cat. 560615, BD Biosciences) and counted with BD LSRFortessa Cytometry (BD Biosciences).
Lee G.J., Jun Y., Yoo H.Y., Jeon Y.K., Lee D., Lee S, & Kim J. (2020). Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation. Oncoimmunology, 9(1), 1746553.
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2

Multiparametric Flow Cytometry Analysis

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Single-cell suspensions were washed and stained with Fixable Near-IR Dead Cell Stain Kit (Thermo Fisher, L10119) followed by staining of extracellular antigens with fluorochrome labelled antibodies. The Fixation/Permeabilization Solution Kit (BD Biosciences, 554714) was used for exposing cytoplasmic antigens. The Transcription Factor Buffer Set (BD Biosciences, 562574, RRID : AB_2869424) was used for exposing nuclear antigens. Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53- 6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher. Cell counting was performed with CountBright Absolute Counting Beads (Thermo Fisher, C36950). Samples were processed in a FACS Canto II flow cytometer (BD Biosciences). Data analysis was performed with FlowJo (RRID : SCR_008520, version 10.7.2).
Barbieri L., Veliça P., Gameiro P.A., Cunha P.P., Foskolou I.P., Rullman E., Bargiela D., Johnson R.S, & Rundqvist H. (2023). Lactate exposure shapes the metabolic and transcriptomic profile of CD8+ T cells. Frontiers in Immunology, 14, 1101433.
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3

Multi-panel Flow Cytometry Immunophenotyping

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Flow cytometry experiments were performed on Aurora (Cytek Biosciences), BD Symphony 3 Flow cytometer, BD FACS ARIA III Sorter, and BD Melody Cell Sorter from the flow core in the University of Pittsburgh or Center for Discovery and Innovation and analyzed by Flowjo (BD). CD45 (clone 30-F11), CD4 (clone RMT4-5), CD8a (clone 53.67), Tcf1/Tcf7 (clone C63D9), PD-1 (clone J43), Tim-3 (clone RMT3-23), Lag-3 (clone C9B7W), Thy1.2 (clone 53-2.1), IFN-γ (clone XMG1.2), Ly-6C (clone HK1.4), Ly-6G (clone 1A8), Sca-1 (clone D7), ST2 (clone RMST2-33), CD103 (clone M290), GzmB (clone QA16A02), Foxp3 (clone MF-14), Ki-67 (clone 16A8), CD11b (clone M1/70), CD11c (clone N418), CD86 (clone GL-1), MHCII (clone), F4/80 (clone BM8), CD206 (clone C068C2), Arg1 (clone A1exF5), CD25 (clone PC61), CD62L (clone MEL-14), CD44 (clone IM7), CD90.2 (clone 53-2.1), CD140a (clone APA5), LIVE/DEAD dye (Zombie NIR Dye) were purchased from BD Bioscience, Thermo Fisher Scientific, or BioLegend. For intracellular transcription factors and cytokines staining, cells were stimulated with a leukocyte activation cocktail (BD) for 6 hours and then followed the standard staining protocol described previously (49 ).
Sun R., Zhao H., Gao D.S., Ni A., Li H., Chen L., Lu X., Chen K, & Lu B. (2023). Amphiregulin couples IL1RL1+ regulatory T cells and cancer-associated fibroblasts to impede antitumor immunity. Science Advances, 9(34), eadd7399.
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4

Multiparametric analysis of immune cells

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Single cell suspensions were prepared from lung allografts, draining mediastinal lymph nodes and spleens as previously described and incubated with CD16/32 antibody 30 minutes prior to the staining with fluorochrome-labeled antibodies specific for CD90.2 (clone 30-H12), CD90.1 (clone HIS51), CD62L (clone MEL-14), CD44 (clone IM7), CD8a (clone 53-6.7), Foxp3 (clone FJK-16s), CD4 (clone RM4-5), CD11c (clone N418), CD45.2 (clone 104), CD45.1 (clone A20) (all from ebioscience, San Diego, CA) PD-L1 (clone MIH5) and PD-1 (clone J43) (BD Pharmingen, San Jose, CA) (2 (link)).
Takahashi T., Hsiao H., Tanaka S., Li W., Higashikubo R., Scozzi D., Bharat A., Ritter J., Krupnick A., Gelman A, & Kreisel D. (2017). PD-1 expression on CD8+ T cells regulates their differentiation within lung allografts and is critical for tolerance induction. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 18(1), 216-225.
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