Glutamax

Manufactured by Miltenyi Biotec

Sourced in Germany

Glutamax is a lab equipment product manufactured by Miltenyi Biotec. It is designed to assist in the cultivation of cells by providing a source of glutamine, an essential amino acid. The core function of Glutamax is to supplement cell culture media with glutamine, which is necessary for cellular growth and metabolism.

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Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Click s media, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Thp 1, by American Type Culture Collection (1 mentions) Interleukin 2 (il 2), by Miltenyi Biotec (1 mentions) Daudi, by American Type Culture Collection (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Cultrex mouse laminin 1, by R&D Systems (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Penicillin streptomycin, by Miltenyi Biotec (1 mentions) Human m csf, by Miltenyi Biotec (1 mentions) Je6 elutriator, by Beckman Coulter (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) 5 n ethylcarboxamidoadenosine neca, by Merck Group (1 mentions) Cytometric bead array technology, by BD (1 mentions) Texmacs, by Miltenyi Biotec (1 mentions)

4 protocols using glutamax

Cell Line Maintenance and T-Cell Culture

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293T (HEK 293 T/17), Raji, Daudi, and THP-1 cell lines were obtained from the American Type Culture Collection. Cell lines were maintained in DMEM (Invitrogen, Grand Island, NY) supplemented with 10% fetal calf serum (FCS) and 2 mM GlutaMAX™ (Invitrogen) at 37 °C and 5% CO₂. T cells generated from peripheral blood mononuclear cells (PBMC) were cultured in 45% RPMI 1640, 45% Click’s media (Invitrogen) supplemented with 10% fetal bovine serum (FBS), 2 mM GlutaMAX (T-cell media, TCM), and 100 U/ml IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), unless otherwise noted. Clinical-grade rimiducid (5 mg/ml in 25% Kolliphor HS15^®) was diluted in ethanol to a 100 mM working solution for in vitro assays, or 0.9% saline for animal studies.

Collinson-Pautz M.R., Chang W.C., Lu A., Khalil M., Crisostomo J.W., Lin P.Y., Mahendravada A., Shinners N.P., Brandt M.E., Zhang M., Duong M., Bayle J.H., Slawin K.M., Spencer D.M, & Foster A.E. (2019). Constitutively active MyD88/CD40 costimulation enhances expansion and efficacy of chimeric antigen receptor T cells targeting hematological malignancies. Leukemia, 33(9), 2195-2207.

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Myelination of Rat and Mouse DRGs

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DRGs were excised from E14 Sprague-Dawley (SD) rat or E12 mouse embryos. The entire ganglia were plated on 24 well plates coated with poly-L lysine (PLL) (Sigma Aldrich) and cultrex mouse laminin I (R&D systems), and cultured with MACS ^R Neuro Medium (Miltenyi Biotec) supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich), 1:200 Glutamax (Thermo Fischer Scientific), 50 U/ml Penicillin/Streptomycin (Thermo Fischer Scientific) and 100ng/ml 2.5S nerve growth factor (NGF) (COSMO BIO). Sixteen hours later, the medium was changed to MACS ^R Neuro Medium supplemented with 1% MACS^R NeuroBrew^R-B21 (Miltenyi Biotec), 1:200 Glutamax, 50 U/ml Penicillin/Streptomycin and 100 ng/ml 2.5S NGF. At day 5, the medium was replaced DMEM supplemented with 10% FBS, 1:200 Glutamax, 50 U/ml Penicillin/Streptomycin and 100 ng/ml 2.5S NGF, and thereafter half of the medium was changed every other day. On day14, 50 μg/ml of L-ascorbic acid (Sigma Aldrich) was added to the medium to initiate myelination. For EM analysis, DRGs were cultured on Cell Disk LF (Sumitomo Bakelite Co.) coated PLL and cultrex mouse laminin I.

Numata-Uematasu Y., Wakatsuki S., Kobayashi-Ujiie Y., Sakai K., Ichinohe N, & Araki T. (2023). In vitro myelination using explant culture of dorsal root ganglia: An efficient tool for analyzing peripheral nerve differentiation and disease modeling. PLOS ONE, 18(5), e0285897.

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Monocyte-Derived Macrophage Isolation

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Human PBMCs were isolated from a single blood transfusion donor, using Ficoll-Hypaque density centrifugation of leukocytes from a leukoreduction system chamber (NHS blood transfusion service). Subsequently, lymphocytes and monocytes were separated in a JE6 elutriator (Beckman Coulter), and monocytes were differentiated into macrophages on glass coverslips (SLS) in 5 days, using RPMI 1640 medium containing 100 ng/mL human MCSF (Miltenyi Biotec), next to 10% heat-inactivated FCS, 4 mmol/L Glutamax, 5 mmol/L sodium pyruvate, and antibiotics (Gibco, Thermo Fisher Scientific).

Engelen S.E., Ververs F.A., Markovska A., Lagerholm B.C., Kraaijenhof J.M., Yousif L.I., Zurke Y.X., Gulersonmez C.M., Kooijman S., Goddard M., van Eijkeren R.J., Jervis P.J., Besra G.S., Haitjema S., Asselbergs F.W., Kalkhoven E., Ploegh H.L., Boes M., Cerundolo V., Hovingh G.K., Salio M., Stigter E.C., Rensen P.C., Monaco C, & Schipper H.S. (2023). Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T cells. JCI Insight, 8(9), e158089.

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Expanded NK Cell Cytokine Secretion

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FACS Aria-sorted NK cells from the spleen (Lin neg, 7AAD neg , CD49a neg , NKp46 + , NK1.1 + , CD49b + ) of the indicated mouse genotypes, were expanded for up to 10 days in complete RPMI 1640 containing 10% FBS, 1 µg/ml of anti-TGF-b1,2,3 blocking antibody (clone 1D11.16.8 from Bio X Cell, West Lebanon, NH), b-Me, GlutaMax and sodium pyruvate (Gibco). For cytokine secretion assays, cells were stimulated in the indicated concentrations of rTGF-b1, rActivin-A, or rIL-15 culture conditions in animal free / TGF-b1 free TexMACS medium (Miltenyi Biotec) containing b-Me, GlutaMax and sodium pyruvate for 48 hours. In certain proliferation assays, the pan adenosine receptor agonist 5'-(N-Ethylcarboxamido) adenosine (NECA) (Sigma Aldrich, St Louis. MO) was used at 1 µM in the culture media for comparison with TGF-b1. For the detection of cytokines in the supernatants of in vitro assays, IFN-g was measured by ELISA with the respective human or murine IFN-g Duoset Kit (R&D Systems) according to the manufacturer's instructions, while all other indicated cytokines were detected using Cytometric Bead Array (CBA) technology (BD Biosciences) according to the manufacturer's instructions.

Rautela J., Dagley L.F., de Oliveira C.C., Schuster I.S., Hediyeh-Zadeh S., Delconte R.B., Cursons J., Hennessy R., Hutchinson D.S., Harrison C., Kita B., Vivier E., Webb A.I., Degli-Esposti M.A., Davis M.J., Huntington N.D, & Souza-Fonseca-Guimaraes F. (2019). Therapeutic blockade of activin-A improves NK cell function and antitumor immunity. Science signaling, 12(596).

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